The Chlamydomonas Sourcebook: Organellar and Metabolic Processes

This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. * Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism * Includes contributions from leaders in particular areas of research * Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media * Assists researchers with common laboratory problems such as contamination * Includes valuable student demonstrations and properties of particular strains and mutants * Edited by the leading researcher in Chlamydomonas science

This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. * Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism * Includes contributions from leaders in particular areas of research * Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media * Assists researchers with common laboratory problems such as contamination * Includes valuable student demonstrations and properties of particular strains and mutants * Edited by the leading researcher in Chlamydomonas science

During the last ten years, knowledge about the multitude of adaptive responses of plants to low oxygen stress has grown immensely. The oxygen sensor mechanism has been discovered, the knowledge about the interaction network of gene expression is expanding and metabolic adaptations have been described in detail. Furthermore, morphological changes were investigated and the regulative mechanisms triggered by plant hormones or reactive oxygen species have been revealed. This book provides a broad overview of all these aspects of low oxygen stress in plants. It integrates knowledge from different disciplines such as molecular biology, biochemistry, ecophysiology and agricultural / horticultural sciences to comprehensively describe how plants cope with low oxygen stress and discuss its ecological and agronomical consequences. This book is written for plant scientists, biochemists and scientists in agriculture and ecophysiology.

This book summarizes recent advances in understanding the functions of plant and algal lipids in photosynthesis, in development and signaling, and in industrial applications. As readers will discover, biochemistry, enzymology and analytical chemistry, as well as gene knock-out studies have all contributed to our rapidly increasing understanding of the functions of lipids. In the past few decades, distinct physical and biochemical properties of specific lipid classes were revealed in plant and algal lipids and the functional aspects of lipids in modulating critical biological processes have been uncovered. These chapters from international authors across relevant research fields highlight the underlying evolutionary context of lipid function in photosynthetic unicellular and multicellular organisms. The book goes on to encompass what lipids can do for industrial applications at a time of fascination with plants and algae in carbon fixation and as sources for production of food, energy and novel chemicals. The developmental context is a part of the fresh and engaging perspective that is presented in this work which graduate students and scientists will find both illuminating and useful.

This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in the field Cutting-edge material

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Expanded revision of gold standard reference Includes latest advances in research, including completion of the genome Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields Available as part of a 3-Volume Set or sold individually

The first comprehensive single-authored textbook on genome-scale models and the bottom-up approach to systems biology.

This volume reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport, and more will use this reference as a guide for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a Website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process Essential reference to a model species for the study of mechanisms of motility in free living cells Includes methods for Chlamydomonas motility research Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action

Freshwater Algae of North America: Ecology and Classification, Second Edition is an authoritative and practical treatise on the classification, biodiversity, and ecology of all known genera of freshwater algae from North America. The book provides essential taxonomic and ecological information about one of the most diverse and ubiquitous groups of organisms on earth. This single volume brings together experts on all the groups of algae that occur in fresh waters (also soils, snow, and extreme inland environments). In the decade since the first edition, there has been an explosion of new information on the classification, ecology, and biogeography of many groups of algae, with the use of molecular techniques and renewed interest in biological diversity. Accordingly, this new edition covers updated classification information of most algal groups and the reassignment of many genera and species, as well as new research on harmful algal blooms. Extensive and complete Describes every genus of freshwater algae known from North America, with an analytical dichotomous key, descriptions of diagnostic features, and at least one image of every genus. Full-color images throughout provide superb visual examples of freshwater algae Updated Environmental Issues and Classifications, including new information on harmful algal blooms (HAB) Fully revised introductory chapters, including new topics on biodiversity, and taste and odor problems Updated to reflect the rapid advances in algal classification and taxonomy due to the widespread use of DNA technologies

Provides a thorough overview of current research with the green alga Chlamydomonas on chloroplast and mitochondrial biogenesis and function, with an emphasis on the assembly and structure-function relationships of the constituents of the photosynthetic apparatus. Contributions emphasize the multidisciplinary nature of current research in photosynthesis, combining molecular genetics, biochemical, biophysical, and physiological approaches. The 36 articles address topics including nuclear genome organization; RNA stability and processing; splicing; translation; protein targeting in the chloroplast; photosystems; pigments; glycerolipids; the ATP synthase; and ferrodoxin and thioredoxin. Further contributions address new measurements methods for photosynthetic activity in vivo; starch biosynthesis; the responses of Chlamydomonas to various stress conditions; nitrogen assimilation; and mitochondrial genetics. Annotation copyrighted by Book News, Inc., Portland, OR

This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Powerful new strategies in functional genomic and genetics combined with biochemical and physiological analyses revealed new insights into Chlamydomonas biology.

The green alga Chlamydomonas is widely used as an experimental model system for studies in cellular and molecular biology, and in particular plant molecular biology. This book is the only single modern compendium of information on its biology and in particular its molecular biology and genetics. Included in addition to much information on the basic biology is material of a very practical nature, namely, methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. Casual users as well as specialists will find the book to be useful in many ways. Key Features * Provides access to previously unpublished data from genetic analysis * Provides descriptions of mutant strains * Depicts summary tables comparing properties of different species and their mutant strains * Explains detailed methods for laboratory procedures of general utility * Furnishes comparisons of culture media * Presents lists of inhibitors, mutagens, and other additives to culture media * Assists with common laboratory problems such as contamination and storage of strains * Demonstrates protocols for laboratory demonstrations available for undergraduate teaching.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers cilia Contains chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins

This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Advancement in Chlamydomonas biology allowed new understandings in biotechnological and biomedical related aspects.

Case Studies in Cell Biology presents real world scenarios to help readers use science process and reasoning skills. The case studies require application and analyzation of concepts beyond rote memory of biological concepts. The book is based on the student learning outcomes from the American Society for Cell Biology, offering practical application for both the classroom and research laboratory. Guides the reader in applying knowledge directly to real world scenarios Includes case studies to bridge foundational cell biological concepts to translational science Aids students in synthesizing information and applying science processes

The Evolution of the Genome provides a much needed overview of genomic study through clear, detailed, expert-authored discussions of the key areas in genome biology. This includes the evolution of genome size, genomic parasites, gene and ancient genome duplications, polypoidy, comparative genomics, and the implications of these genome-level phenomena for evolutionary theory. In addition to reviewing the current state of knowledge of these fields in an accessible way, the various chapters also provide historical and conceptual background information, highlight the ways in which the critical questions are actually being studied, indicate some important areas for future research, and build bridges across traditional professional and taxonomic boundaries. The Evolution of the Genome will serve as a critical resource for graduate students, postdoctoral fellows, and established scientists alike who are interested in the issue of genome evolution in the broadest sense. Provides detailed, clearly written chapters authored by leading researchers in their respective fields Presents a much-needed overview of the historical and theoretical context of the various areas of genomic study Creates important links between topics in order to promote integration across subdisciplines, including descriptions of how each subject is actually studied Provides information specifically designed to be accessible to established researchers, postdoctoral fellows, and graduate students alike

Microbial Diversity in the Genomic Era presents insights on the techniques used for microbial taxonomy and phylogeny, along with their applications and respective pros and cons. Though many advanced techniques for the identification of any unknown bacterium are available in the genomics era, a far fewer number of the total microbial species have been discovered and identified to date. The assessment of microbial taxonomy and biosystematics techniques discovered and practiced in the current genomics era with suitable recommendations is the prime focus of this book. Discusses the techniques used for microbial taxonomy and phylogeny with their applications and respective pros and cons Reviews the evolving field of bacterial typing and the genomic technologies that enable comparative analysis of multiple genomes and the metagenomes of complex microbial environments Provides a uniform, standard methodology for species designation