The Chlamydomonas Sourcebook: Organellar and Metabolic Processes

This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. * Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism * Includes contributions from leaders in particular areas of research * Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media * Assists researchers with common laboratory problems such as contamination * Includes valuable student demonstrations and properties of particular strains and mutants * Edited by the leading researcher in Chlamydomonas science

This second volume of The Chlamydomonas Sourcebook provides the background and techniques for using this important organism in plant research. From biogenesis of chloroplasts and mitochondria and photosynthesis to respiration and nitrogen assimilation, this volume introduces scientists to the functions of the organism. The volume then moves on to starch biosynthesis, sulfur metabolism, response to heavy metals, and hydrogen production. * Describes molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism * Includes contributions from leaders in particular areas of research * Provides methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media * Assists researchers with common laboratory problems such as contamination * Includes valuable student demonstrations and properties of particular strains and mutants * Edited by the leading researcher in Chlamydomonas science

During the last ten years, knowledge about the multitude of adaptive responses of plants to low oxygen stress has grown immensely. The oxygen sensor mechanism has been discovered, the knowledge about the interaction network of gene expression is expanding and metabolic adaptations have been described in detail. Furthermore, morphological changes were investigated and the regulative mechanisms triggered by plant hormones or reactive oxygen species have been revealed. This book provides a broad overview of all these aspects of low oxygen stress in plants. It integrates knowledge from different disciplines such as molecular biology, biochemistry, ecophysiology and agricultural / horticultural sciences to comprehensively describe how plants cope with low oxygen stress and discuss its ecological and agronomical consequences. This book is written for plant scientists, biochemists and scientists in agriculture and ecophysiology.

The first comprehensive single-authored textbook on genome-scale models and the bottom-up approach to systems biology.

Lipids in Photosynthesis: Essential and Regulatory Functions, provides an essential summary of an exciting decade of research on relationships between lipids and photosynthesis. The book brings together extensively cross-referenced and peer-reviewed chapters by prominent researchers. The topics covered include the structure, molecular organization and biosynthesis of fatty acids, glycerolipids and nonglycerolipids in plants, algae, lichens, mosses, and cyanobacteria, as well as in chloroplasts and mitochondria. Several chapters deal with the manipulation of the extent of unsaturation of fatty acids and the effects of such manipulation on photosynthesis and responses to various forms of stress. The final chapters focus on lipid trafficking, signaling and advanced analytical techniques. Ten years ago, Siegenthaler and Murata edited "Lipids in Photosynthesis: Structure, Function and Genetics," which became a classic in the field. "Lipids in Photosynthesis: Essential and Regulatory Functions," belongs, with its predecessor, in every plant and microbiological researcher's bookcase.

This book summarizes recent advances in understanding the functions of plant and algal lipids in photosynthesis, in development and signaling, and in industrial applications. As readers will discover, biochemistry, enzymology and analytical chemistry, as well as gene knock-out studies have all contributed to our rapidly increasing understanding of the functions of lipids. In the past few decades, distinct physical and biochemical properties of specific lipid classes were revealed in plant and algal lipids and the functional aspects of lipids in modulating critical biological processes have been uncovered. These chapters from international authors across relevant research fields highlight the underlying evolutionary context of lipid function in photosynthetic unicellular and multicellular organisms. The book goes on to encompass what lipids can do for industrial applications at a time of fascination with plants and algae in carbon fixation and as sources for production of food, energy and novel chemicals. The developmental context is a part of the fresh and engaging perspective that is presented in this work which graduate students and scientists will find both illuminating and useful.

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Expanded revision of gold standard reference Includes latest advances in research, including completion of the genome Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields Available as part of a 3-Volume Set or sold individually

This volume reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport, and more will use this reference as a guide for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a Website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility. This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually. Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process Essential reference to a model species for the study of mechanisms of motility in free living cells Includes methods for Chlamydomonas motility research Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action

Case Studies in Cell Biology presents real world scenarios to help readers use science process and reasoning skills. The case studies require application and analyzation of concepts beyond rote memory of biological concepts. The book is based on the student learning outcomes from the American Society for Cell Biology, offering practical application for both the classroom and research laboratory. Guides the reader in applying knowledge directly to real world scenarios Includes case studies to bridge foundational cell biological concepts to translational science Aids students in synthesizing information and applying science processes

DNA methylation is essential for the normal development and functioning of organisms. This volume discusses the latest developments in this very active field of research. It presents the evolution of DNA methylation, mammalian DNA methyltransferases, DNA methylation and demethlyation, DNA methylation and silencing and the role it plays in medicine including cancer. * Discusses new discoveries, approaches, and ideas * Contributions from leading scholars and industry experts * Reference guide for researchers involved in molecular biology and related fields

This book presents in an easy-to-read format a summary of the important central aspects of microbial glycobiology, i.e. the study of carbohydrates as related to the biology of microorganisms. Microbial glycobiology represents a multidisciplinary and emerging area with implications for a range of basic and applied research fields, as well as having industrial, medical and biotechnological implications. Individual chapters provided by leading international scientists in the field yield insightful, concise and stimulating reviews Provides researchers with an overview and synthesis of the latest research Each chapter begins with a brief 200 word Summary/Abstract detailing the topic and focus of the chapter, as well as the concepts to be addressed Allows researchers to see at a glance what each chapter will cover Each chapter includes a Research Focus Box Identifies important problems that still need to be solved and areas that require further investigation

Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides presents a comprehensive, systematic and authoritative survey of information about a family of chemically related, but functionally diverse, naturally occurring polysaccharides--the (1-3)-glucans. International contributors describe the chemical and physicochemical properties of these glucans and their derivatives and the molecular biological and structural aspects of the enzymes involved in their formation and breakdown. A detailed analysis of their physiological roles in the various biological situations in which they are found will be provided. Additionally, evolutionary relationships among the family of these glucans will be described. Topics of medical relevance include detailing the glucans' interactions with the immune system and research for cancer therapy applications Web resource links allow scientists to explore additional beta glucan research Separate indexes divided into Species and Subject for enhanced searchability

This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Powerful new strategies in functional genomic and genetics combined with biochemical and physiological analyses revealed new insights into Chlamydomonas biology.

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers cilia Contains chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins

This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Advancement in Chlamydomonas biology allowed new understandings in biotechnological and biomedical related aspects.

Provides a thorough overview of current research with the green alga Chlamydomonas on chloroplast and mitochondrial biogenesis and function, with an emphasis on the assembly and structure-function relationships of the constituents of the photosynthetic apparatus. Contributions emphasize the multidisciplinary nature of current research in photosynthesis, combining molecular genetics, biochemical, biophysical, and physiological approaches. The 36 articles address topics including nuclear genome organization; RNA stability and processing; splicing; translation; protein targeting in the chloroplast; photosystems; pigments; glycerolipids; the ATP synthase; and ferrodoxin and thioredoxin. Further contributions address new measurements methods for photosynthetic activity in vivo; starch biosynthesis; the responses of Chlamydomonas to various stress conditions; nitrogen assimilation; and mitochondrial genetics. Annotation copyrighted by Book News, Inc., Portland, OR

The Molecular Nutrition of Amino Acids and Proteins provides an in-depth look at the involvement and role of amino acids and proteins in molecular nutrition. Editor Dominique Dardevet has assembled a collection of chapters written by leading researchers and top professors that provide the reader with a comprehensive understanding of amino acids and proteins. The book provides an introduction to the fundamentals of amino acids and proteins as well as the composition of food. It then delves into the molecular biology of the cell and genetic machinery and its function. The Molecular Nutrition of Amino Acids and Proteins also features reference guides for terms and bullet-point summaries, making it readily accessible to novices while still providing the most up-to-date and detailed information that experienced researchers need. Provides a gentle introduction to the subject by first addressing nutritional information and then building in molecular aspects, clearly establishing fundamental information for the reader Facilitates reader comprehension by including succinct summary points in each chapter Contains a glossary of definitions that allows readers to easily reference terms Provides both a deep and broad understanding of the subject by containing overviews as well as detail-focused chapters

In this volume of Cell and Molecular Responses to Stress articles provide up-to-date information on key areas of signal sensing (sensing of pain, heat, cold, light, infrared radiation), molecules involved in the intracellular transmission of these signals, metabolic responses to stress including changes in gene expression and production of specialized proteins that aid cell responses to factors including interrupted blood supply (ischemia), oxygen limitation (hypoxia/anoxia), freezing and dehydration, amino acid limitation, radiation and processing drugs. There are chapters which also provide insights into new technologies (such as cDNA arrays), analysis of metabolic control theory (a key method for analysing stress effects on cells), and examine how enzymes evolve in the face of stress.