RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell, Jr.
Publsiher: Academic Press
Total Pages: 744
Release: 2009-08-31
ISBN 10: 9780080884950
ISBN 13: 0080884954
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Review:

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell, Jr.
Publsiher: Elsevier
Total Pages: 688
Release: 2010-07-22
ISBN 10: 0080454763
ISBN 13: 9780080454764
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Review:

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell
Publsiher: Academic Press
Total Pages: 767
Release: 2005
ISBN 10: 0122496965
ISBN 13: 9780122496967
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Review:

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell
Publsiher: Academic Press
Total Pages: 767
Release: 2005
ISBN 10: 0122496965
ISBN 13: 9780122496967
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Review:

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

RNA Methodologies
Author: Gerard Meurant
Publsiher: Academic Press
Total Pages: 317
Release: 2012-12-02
ISBN 10: 0323137792
ISBN 13: 9780323137799
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Review:

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA

RNA ligand Interactions Structural biology methods Semisynthetic methodologies RNA structure determination Techniques for monitoring RNA conformation and dynamics Modeling tertiary structure

RNA ligand Interactions  Structural biology methods  Semisynthetic methodologies   RNA structure determination   Techniques for monitoring RNA conformation and dynamics   Modeling tertiary structure
Author: Daniel Walter Celander,John Abelson
Publsiher: Anonim
Total Pages: 329
Release: 2000
ISBN 10:
ISBN 13: UVA:X004346010
Language: EN, FR, DE, ES & NL

RNA ligand Interactions Structural biology methods Semisynthetic methodologies RNA structure determination Techniques for monitoring RNA conformation and dynamics Modeling tertiary structure Book Review:

Epigenetic Regulation and Epigenomics

Epigenetic Regulation and Epigenomics
Author: Robert A. Meyers
Publsiher: John Wiley & Sons
Total Pages: 1254
Release: 2012-10-02
ISBN 10: 3527668624
ISBN 13: 9783527668625
Language: EN, FR, DE, ES & NL

Epigenetic Regulation and Epigenomics Book Review:

Epigenetics is a term in biology referring to heritable traits that do not involve changes in the underlying DNA sequence of the organism. Epigenetic traits exist on top of or in addition to the traditional molecular basis for inheritance. The "epigenome" is a parallel to the word "genome," and refers to the overall epigenetic state of a cell. Cancer and stem cell research have gradually focused attention on these genome modifications. The molecular basis of epigenetics involves modifications to DNA and the chromatin proteins that associate with it. Methylation, for example, can silence a nearby gene and seems to be involved in some cancers. Epigenetics is beginning to form and take shape as a new scientific discipline, which will have a major impact on Medicine and essentially all fields of biology. Increasingly, researchers are unearthing links between epigenetics and a number of diseases. Although in recent years cancer has been the main focus of epigenetics, recent data suggests that epigenetic plays a critical role in psychology and psychopathology. It is being realized that normal behaviors such as maternal care and pathologies such as Schizophrenia and Alzheimer's might have an epigenetic basis. It is also becoming clear that nutrition and life experiences have epigenetic consequences. Discover more online content in the Encyclopedia of Molecular Cell Biology and Molecular Medicine.

Methodologies and Results in Grapevine Research

Methodologies and Results in Grapevine Research
Author: Serge Delrot,Hipólito Medrano,Etti Or,Luigi Bavaresco,Stella Grando
Publsiher: Springer Science & Business Media
Total Pages: 448
Release: 2010-10-19
ISBN 10: 9789048192830
ISBN 13: 9048192838
Language: EN, FR, DE, ES & NL

Methodologies and Results in Grapevine Research Book Review:

Grapevine is a crop of major economical interest, and wine represents a multicultural heritage which has been growing since several milleniums. Yet, modern viticulture must face several challenges. Global climate has increased berry sugar content (and alcohol in the wine) whereas phenolic and aromatic ripeness are not always achieved. Water supply is becoming shorter. New varieties better adapted to new climatic conditions might have to be planted, which may affect wine typicity. Phytochemical treatments are more controlled, and the consumer pays increasing attention to environmentally safe practices. New methods reducing pesticide use, but maintaining yield and typicity, must be designed. The present book illustrates the recent progress made in ecophysiology, molecular and cell biology, and pathology of grapevine, as well as in precision viticulture and berry composition. Combination of these new tools with field observations will undoubtly make it easier to face the challenges described above. These multidisciplinary contributions will be of interest to anyone involved in grapevine and wine activities.

Trends in RNA Research

Trends in RNA Research
Author: Peter A. McNamara
Publsiher: Nova Publishers
Total Pages: 315
Release: 2006
ISBN 10: 9781594545061
ISBN 13: 1594545065
Language: EN, FR, DE, ES & NL

Trends in RNA Research Book Review:

RNA is a chemical found in the nucleus and cytoplasm of cells. RNA plays an important role in protein synthesis and other chemical activities of the cell. The structure of RNA is similar to that of DNA, although RNA is single stranded whereas DNA has a characteristic "double helix". Genetic information is stored by DNA in the nucleus of cells, and RNA carries that information to other parts of the cell where it is converted into protein. Three types of RNA are; mRNA (messenger RNA, which contains the specific sequence of nucleotides necessary to dictate amino acid sequence in proteins), tRNA (transfer RNA, which serves as the "adapter" to position the appropriate amino acid next to a growing polypeptide chain during protein synthesis), and rRNA (ribosomal RNA, which is the RNA component of ribosomes). This book presents state-of-the-research from throughout the world.

Combinatorics Algorithms Probabilistic and Experimental Methodologies

Combinatorics  Algorithms  Probabilistic and Experimental Methodologies
Author: Bo Chen
Publsiher: Springer Science & Business Media
Total Pages: 530
Release: 2007-09-28
ISBN 10: 3540744495
ISBN 13: 9783540744498
Language: EN, FR, DE, ES & NL

Combinatorics Algorithms Probabilistic and Experimental Methodologies Book Review:

The First International Symposium on Combinatorics, Algorithms, Probabilistic and Experimental Methodologies was held in Hangzhou, China, in April 2007. The symposium provided an interdisciplinary forum for researchers to share their discoveries and approaches; search for ideas, methodologies, and tool boxes; find better, faster, and more accurate solutions; and develop a research agenda of common interest. This volume constitutes the refereed post-proceedings of the symposium. Inside you'll find 46 full papers. They represent some of the most important thinking and advancements in the field. The papers address large data processing problems using different methodologies from major disciplines such as computer science, combinatorics, and statistics.

Recombinant DNA Principles and Methodologies

Recombinant DNA Principles and Methodologies
Author: James Greene
Publsiher: CRC Press
Total Pages: 768
Release: 1998-06-25
ISBN 10: 9780824799892
ISBN 13: 0824799895
Language: EN, FR, DE, ES & NL

Recombinant DNA Principles and Methodologies Book Review:

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies! Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology nucleic acid biochemistry restriction enzymes cloning strategies gel electrophoresis southern and northern blotting preparation of probes phage lambda biology cosmids and genome analysis cloned gene expression polymerase chain reaction conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

RNA seq Data Analysis

RNA seq Data Analysis
Author: Eija Korpelainen,Jarno Tuimala,Panu Somervuo,Mikael Huss,Garry Wong
Publsiher: CRC Press
Total Pages: 322
Release: 2014-09-19
ISBN 10: 1466595019
ISBN 13: 9781466595019
Language: EN, FR, DE, ES & NL

RNA seq Data Analysis Book Review:

The State of the Art in Transcriptome AnalysisRNA sequencing (RNA-seq) data offers unprecedented information about the transcriptome, but harnessing this information with bioinformatics tools is typically a bottleneck. RNA-seq Data Analysis: A Practical Approach enables researchers to examine differential expression at gene, exon, and transcript le

Advancing Cryptosporidium Parvum Detection Methodologies

Advancing Cryptosporidium Parvum Detection Methodologies
Author: Mark C. Jenkins
Publsiher: American Water Works Association
Total Pages: 68
Release: 2002
ISBN 10: 9781583212660
ISBN 13: 1583212663
Language: EN, FR, DE, ES & NL

Advancing Cryptosporidium Parvum Detection Methodologies Book Review:

This study focused on ideal methods of detecting C. parvum oocysts which give low detection limits, specific and sensitive detection in concentraded samples, and differentiation of viable from non-viable oocysts; are usable by a water treatment facility and provide quantitative results within a few hours. Potential useful methods are continuous flow centrifugation, rel-time TaqMan PCR, cell culture inoculation followed by PCR of DNA extration, and FISH.

Epitranscriptomics The Novel RNA Frontier

Epitranscriptomics  The Novel RNA Frontier
Author: Giovanni Nigita,Mario Acunzo,William Chi Shing Cho,Carlo Maria Croce
Publsiher: Frontiers Media SA
Total Pages: 127
Release: 2019-01-31
ISBN 10: 2889457419
ISBN 13: 9782889457410
Language: EN, FR, DE, ES & NL

Epitranscriptomics The Novel RNA Frontier Book Review:

Following the formulation of the central dogma of molecular biology and the later discovery of classes of non-coding RNAs, the primary focus of Genetics was essentially on variation of DNA aiming at elucidating biological pathways perturbed in diseases. Recently, extensive attention has shifted towards the study of posttranscriptional RNA modifications occurring in both protein-coding as well as non-coding RNAs, revealing a novel and finer layer of complexity in gene regulation. This, in turn, has led to the birth of the novel field of ‘Epitranscriptomics’. The recent increase of applications of high-throughput sequencing technology (HTS) has allowed the unprecedented opportunity to identify on a transcriptome-wide scale, millions of RNA modifications in human genes, counting today more than 140 distinct types such as: methylation (e.g. m6A, m1A, m5C, hm5C, 2’OMe) methylation (e.g. m6A, m1A, m5C, hm5C, 2’OMe), pseudourylation (?), deamination (e.g. A-to-I RNA editing). The scope of this Research Topic was to collect both reviews and research articles addressing the wet lab approaches and bioinformatics methodologies necessary to aid in the identification of novel RNA modifications and characterization of their biological functions. Among the articles embracing the aim of the Research Topic, we have collected four original research and methods articles, five reviews, and a technology article.

RNA Detection

RNA Detection
Author: Imre Gaspar
Publsiher: Anonim
Total Pages: 492
Release: 2018
ISBN 10: 9781493972135
ISBN 13: 1493972138
Language: EN, FR, DE, ES & NL

RNA Detection Book Review:

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

RNA Ligand Interactions Part B Molecular Biology Methods

RNA Ligand Interactions  Part B  Molecular Biology Methods
Author: Anonim
Publsiher: Elsevier
Total Pages: 539
Release: 2000-06-26
ISBN 10: 008049675X
ISBN 13: 9780080496757
Language: EN, FR, DE, ES & NL

RNA Ligand Interactions Part B Molecular Biology Methods Book Review:

RNA-Ligand Interactions, Part B focuses on molecular biology methods. Major topics covered include: solution probe methods, tethered-probe methodologies, in vitro affinity selection methodologies, genetic methodologies for detecting RNA-protein interactions, protein engineering methodologies useful for RNA-protein interaction studies, and cell biology methods. RNA-Ligand Interactions, Part A, its companion, VOLUME 317 focuses on structural biology methods. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Antisense and Ribozyme Methodology

Antisense and Ribozyme Methodology
Author: Ian Gibson
Publsiher: John Wiley & Sons
Total Pages: 92
Release: 2008-09-26
ISBN 10: 3527612521
ISBN 13: 9783527612529
Language: EN, FR, DE, ES & NL

Antisense and Ribozyme Methodology Book Review:

Antisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating viral infections and cancer. This laboratory companion provides detailed information on the potential, advantages and limitations of this methodology. It critically discusses potential pitfalls, presents strategies for choosing targets and delivery systems, so as to allow the selection of the optimum methodology for achieving fast and reliable experimental success with any human or other biological system. For researchers, technicians and advanced graduates in experimental medicine, molecular and cell biology.

Plant RNA Biology

Plant RNA Biology
Author: Dóra Szakonyi,Ana Confraria,Concetta Valerio,Paula Duque,Dorothee Staiger
Publsiher: Frontiers Media SA
Total Pages: 329
Release: 2019-11-18
ISBN 10: 2889630250
ISBN 13: 9782889630257
Language: EN, FR, DE, ES & NL

Plant RNA Biology Book Review:

Discoveries from the past decades revealed that RNA molecules are much more than inert intermediates between the coding DNA sequences and their functional products, proteins. Today, RNAs are recognized as active regulatory molecules influencing gene expression, chromatin organization and genome stability, thus impacting all aspects of plant life including development, growth, reproduction and stress tolerance. Innovations in methodologies, the expanding application of next-generation sequencing technologies, and the creation of public datasets and databases have exposed a new universe of RNA-based mechanisms and led to the discovery of new families of non-coding RNAs, uncovered the large extent of alternative splicing events, and highlighted the potential roles of RNA modifications and RNA secondary structures. Furthermore, considerable advances have been made in identifying RNA-binding and processing factors involved in the synthesis and maturation of different forms of RNA molecules as well as in RNA processing, biochemical modifications or degradation. This Research Topic showcases the broad biological significance of RNAs in plant systems and contains eight original research articles, one review and four mini-reviews, covering various RNA-based mechanisms in higher plants. Emerging new technologies and novel multidisciplinary approaches are empowering the scientific community and will expectedly bring novel insights into our understanding of the mechanisms through which RNA is regulated and regulates biological processes in plant cells.

I A Novel Computational Methodology for Identifying RNA Minimum Sequence Requirements for Ligand Binding and Recognition

I  A Novel Computational Methodology for Identifying RNA Minimum Sequence Requirements for Ligand Binding and Recognition
Author: Peter C. Anderson
Publsiher: Anonim
Total Pages: 344
Release: 2007
ISBN 10:
ISBN 13: WISC:89096163456
Language: EN, FR, DE, ES & NL

I A Novel Computational Methodology for Identifying RNA Minimum Sequence Requirements for Ligand Binding and Recognition Book Review:

The Molecular Biology of Neurological Disease

The Molecular Biology of Neurological Disease
Author: Roger N. Rosenberg,A. E. Harding
Publsiher: Butterworth-Heinemann
Total Pages: 276
Release: 2013-10-22
ISBN 10: 148316330X
ISBN 13: 9781483163307
Language: EN, FR, DE, ES & NL

The Molecular Biology of Neurological Disease Book Review:

The Molecular Biology of Neurological Disease reviews advances that have been made in understanding the molecular mechanisms of neurological disorders as well as immediate and future applications of molecular biological techniques to clinical practice. This book explores the molecular genetics of neurological disease such as muscular dystrophy, Joseph disease, and Huntington's disease, along with the mitochondrial genes implicated in such conditions. This text is comprised of 18 chapters and begins by introducing the reader to the basic principles and methods of molecular genetic techniques used in the diagnosis of neurological disease. Attention then turns to several aspects of genetic expression in the brain, including the extent to which the genome is expressed in the brain. The next chapter focuses on the visualization of polyadenylated messenger RNAs in individual cells in mammalian brain using in situ hybridization techniques, combined with immunohistochemical localization of specific proteins and neuropeptides implicated in diseases such as Alzheimer dementia. This book also discusses the molecular biology of chemical synaptic neurotransmission; proteins involved in the regulation of nervous system development; and gene expression in skeletal muscle. This text then concludes with a summary of the ""neurological gene map"" as it stands in the latter part of 1987. This book is intended for physicians who grapple with the problems of neurological disorders on a daily basis, including neurologists, neurologists in training, and those in related fields such as neurosurgery, internal medicine, psychiatry, and rehabilitation medicine.