Plant Small RNA

Plant Small RNA
Author: Praveen Guleria,Vineet Kumar
Publsiher: Academic Press
Total Pages: 660
Release: 2020-02
ISBN 10: 9780128171127
ISBN 13: 012817112X
Language: EN, FR, DE, ES & NL

Plant Small RNA Book Review:

Plant Small RNA: Biogenesis, Regulation and Application describes the biosynthesis of small RNA in plant systems. With an emphasis on the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival, this books presents the basics and most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. In addition, it emphasizes the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival. Final sections cover the most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. Presents foundational information about small RNA biology and regulation in plants Includes small RNA pathway advances Describes the application and scope of small RNA technology for agricultural stability

Plant Genomes

Plant Genomes
Author: Jean-Nicolas Volff
Publsiher: Karger Medical and Scientific Publishers
Total Pages: 145
Release: 2008-01-01
ISBN 10: 3805584911
ISBN 13: 9783805584913
Language: EN, FR, DE, ES & NL

Plant Genomes Book Review:

Recent major advances in the field of comparative genomics and cytogenomics of plants, particularly associated with the completion of ambitious genome projects, have uncovered astonishing facets of the architecture and evolutionary history of plant genomes. The aim of this book was to review these recent developments as well as their implications in our understanding of the mechanisms which drive plant diversity. New insights into the evolution of gene functions, gene families and genome size are presented, with particular emphasis on the evolutionary impact of polyploidization and transposable elements. Knowledge on the structure and evolution of plant sex chromosomes, centromeres and microRNAs is reviewed and updated. Taken together, the contributions by internationally recognized experts present a panoramic overview of the structural features and evolutionary dynamics of plant genomes.This volume of Genome Dynamics will provide researchers, teachers and students in the fields of biology and agronomy with a valuable source of current knowledge on plant genomes.

Plant MicroRNAs

Plant MicroRNAs
Author: Stefan de Folter
Publsiher: Humana Press
Total Pages: 363
Release: 2019-01-31
ISBN 10: 9781493990412
ISBN 13: 1493990411
Language: EN, FR, DE, ES & NL

Plant MicroRNAs Book Review:

This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.

Plant RNA Biology

Plant RNA Biology
Author: Dóra Szakonyi,Ana Confraria,Concetta Valerio,Paula Duque,Dorothee Staiger
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2019-11-18
ISBN 10: 2889630250
ISBN 13: 9782889630257
Language: EN, FR, DE, ES & NL

Plant RNA Biology Book Review:

Discoveries from the past decades revealed that RNA molecules are much more than inert intermediates between the coding DNA sequences and their functional products, proteins. Today, RNAs are recognized as active regulatory molecules influencing gene expression, chromatin organization and genome stability, thus impacting all aspects of plant life including development, growth, reproduction and stress tolerance. Innovations in methodologies, the expanding application of next-generation sequencing technologies, and the creation of public datasets and databases have exposed a new universe of RNA-based mechanisms and led to the discovery of new families of non-coding RNAs, uncovered the large extent of alternative splicing events, and highlighted the potential roles of RNA modifications and RNA secondary structures. Furthermore, considerable advances have been made in identifying RNA-binding and processing factors involved in the synthesis and maturation of different forms of RNA molecules as well as in RNA processing, biochemical modifications or degradation. This Research Topic showcases the broad biological significance of RNAs in plant systems and contains eight original research articles, one review and four mini-reviews, covering various RNA-based mechanisms in higher plants. Emerging new technologies and novel multidisciplinary approaches are empowering the scientific community and will expectedly bring novel insights into our understanding of the mechanisms through which RNA is regulated and regulates biological processes in plant cells.

The Model Legume Medicago truncatula 2 Volume Set

The Model Legume Medicago truncatula  2 Volume Set
Author: Frans J. de Bruijn
Publsiher: John Wiley & Sons
Total Pages: 1264
Release: 2020-01-29
ISBN 10: 1119409160
ISBN 13: 9781119409168
Language: EN, FR, DE, ES & NL

The Model Legume Medicago truncatula 2 Volume Set Book Review:

Fully covers the biology, biochemistry, genetics, and genomics of Medicago truncatula Model plant species are valuable not only because they lead to discoveries in basic biology, but also because they provide resources that facilitate translational biology to improve crops of economic importance. Plant scientists are drawn to models because of their ease of manipulation, simple genome organization, rapid life cycles, and the availability of multiple genetic and genomic tools. This reference provides comprehensive coverage of the Model Legume Medicago truncatula. It features review chapters as well as research chapters describing experiments carried out by the authors with clear materials and methods. Most of the chapters utilize advanced molecular techniques and biochemical analyses to approach a variety of aspects of the Model. The Model Legume Medicago truncatula starts with an examination of M. truncatula plant development; biosynthesis of natural products; stress and M. truncatula; and the M. truncatula-Sinorhizobium meliloti symbiosis. Symbiosis of Medicago truncatula with arbuscular mycorrhiza comes next, followed by chapters on the common symbiotic signaling pathway (CSSP or SYM) and infection events in the Rhizobium-legume symbiosis. Other sections look at hormones and the rhizobial and mycorrhizal symbioses; autoregulation of nodule numbers (AON) in M. truncatula; Medicago truncatula databases and computer programs; and more. Contains reviews, original research chapters, and methods Covers most aspects of the M. truncatula Model System, including basic biology, biochemistry, genetics, and genomics of this system Offers molecular techniques and advanced biochemical analyses for approaching a variety of aspects of the Model Legume Medicago truncatula Includes introductions by the editor to each section, presenting the summary of selected chapters in the section Features an extensive index, to facilitate the search for key terms The Model Legume Medicago truncatula is an excellent book for researchers and upper level graduate students in microbial ecology, environmental microbiology, plant genetics and biochemistry. It will also benefit legume biologists, plant molecular biologists, agrobiologists, plant breeders, bioinformaticians, and evolutionary biologists.

Discovery of Endogenous Plant Small RNAs and Their Role in Trans species Gene Regulation

Discovery of Endogenous Plant Small RNAs and Their Role in Trans species Gene Regulation
Author: Saima Shahid
Publsiher: Unknown
Total Pages: 135
Release: 2017
ISBN 10: 1928374650XXX
ISBN 13: OCLC:1021231551
Language: EN, FR, DE, ES & NL

Discovery of Endogenous Plant Small RNAs and Their Role in Trans species Gene Regulation Book Review:

Endogenous small RNAs (20 - 24 nt) engage in complex regulation of gene expression and thus shape and direct plant development, defense, stress response and the epigenome. Based on their biogenesis and functions, endogenous small RNAs can be divided into many categories and subcategories. MicroRNAs (miRNAs) represent the most well-annotated type of small RNAs that regulate gene expression via transcript cleavage or translational repression. However, MIRNAs only contribute to a minor fraction of all the expressed small RNAs in plants. Small RNA genes other than MIRNAs remain poorly annotated, which limits complete elucidation of their regulatory roles. Furthermore, inconsistent MIRNA discovery methodologies in published studies have resulted in widespread discrepancies among existing annotations. To address these issues, and to improve current understanding of small RNA gene functions, we developed robust methodologies for de novo annotation of plant small RNA genes. Our comprehensive small RNA loci discovery based on deep sequencing data and small RNA biogenesis patterns provided refinement of existing MIRNA annotations and their functions in the basal land plant Physcomitrella patens. We also identified numerous P. patens siRNA loci producing almost equal mixture of 23-24 nt small RNAs, confirming that the heterochromatic siRNA pathway is present in the bryophyte lineage. Our de novo annotation of small RNA genes in Amborella trichopoda, the basal-most lineage of flowering plants, revealed a striking predominance of lineage-specific, intronic 23-24 nt MIRNAs and hairpin RNAs that has not been reported in any plants so far. Most of these non-canonical MIRNAs lacked easily identifiable targets in the transcriptome, suggesting these may have functions other than sequence-dependent targeting. In the monocot rice, 24 nt long intronic miRNAs function in RNA dependent DNA methylation. It is possible that A. trichopoda 23-24 nt MIRNAs function in a similar way, and such non-canonical miRNA pathways may have been retained in specific lineages of flowering plants. At least 19 A. trichopoda miRNA families were broadly conserved across land plants, and most of these also had conserved targets. These findings confirmed the presence of all major small RNA gene classes in the basal lineage of flowering plants, as well as the existence of species-specific diversities in small RNA populations expressed in non-model plants. Finally, we explored the potential exchange of endogenous small RNAs between parasitic plants and their hosts. Parasitic plants intimately connect to their hosts through a specialized feeding organ called haustoria. Bidirectional exchange of thousands of mRNAs between the stem parasite C. campestris and its hosts have been previously reported. Host-induced gene silencing has also been shown in several parasitic species including Cuscuta and Triphysaria versicolor (root parasite). De novo annotation of small RNA genes from C. campestris - A. thaliana associations revealed an unprecedented abundance of 22 nt parasite miRNAs in the haustorial interface. Several of these interface-induced C. campestris miRNAs directed slicing of six host mRNAs and triggered secondary siRNA production specifically in interface. Among these targets, Botrytis Induced Kinase 1 (BIK1) encodes a receptor-like cytoplasmic kinase and functions in in plant immunity. Another target, Sieve-Element-Occlusion-Related 1 (SEOR1) encodes a protein thought to be involved in sealing phloem sieve elements after wounding. Additionally, mRNAs encoding three auxin receptors, TIR1, AFB2, and AFB3 were targeted by a C. campestris miRNA and showed a unique pattern of secondary siRNA production in parasite-host interface. Such secondary siRNA production depended on host machinery for RNA interference. Growth of C. campestris on seor1 mutant significantly increased parasite biomass accumulation compared to wild type. Furthermore, interface-induced parasite miRNA-directed cleavage of host TIR1/AFB was also detected in C. campestris -N. benthamiana. Our findings thus confirm conserved trans-species targeting by C. campestris miRNAs across the haustorial interface, and the potential roles of these miRNAs as virulence factors in plant parasitism.

Abiotic and Biotic Stress in Plants

Abiotic and Biotic Stress in Plants
Author: Arun Shanker,Chitra Shanker
Publsiher: BoD – Books on Demand
Total Pages: 768
Release: 2016-02-17
ISBN 10: 9535122509
ISBN 13: 9789535122500
Language: EN, FR, DE, ES & NL

Abiotic and Biotic Stress in Plants Book Review:

The impact of global climate change on crop production has emerged as a major research priority during the past decade. Understanding abiotic stress factors such as temperature and drought tolerance and biotic stress tolerance traits such as insect pest and pathogen resistance in combination with high yield in plants is of paramount importance to counter climate change related adverse effects on the productivity of crops. In this multi-authored book, we present synthesis of information for developing strategies to combat plant stress. Our effort here is to present a judicious mixture of basic as well as applied research outlooks so as to interest workers in all areas of plant science. We trust that the information covered in this book would bridge the much-researched area of stress in plants with the much-needed information for evolving climate-ready crop cultivars to ensure food security in the future.

Non Coding RNAs in Plants

Non Coding RNAs in Plants
Author: Volker A. Erdmann,Jan Barciszewski
Publsiher: Springer Science & Business Media
Total Pages: 510
Release: 2011-07-20
ISBN 10: 9783642194542
ISBN 13: 3642194540
Language: EN, FR, DE, ES & NL

Non Coding RNAs in Plants Book Review:

In the most recent years, each of the RNA silencing pathways of plants have appeared to generate ncRNAs with dedicated functions, specialized biological activities and specific functional scopes. RNA silencing plays a crucial role in coordinating the expression, stability, protection and inheritance of eukaryotic genomes. It compromises several mechanisms, that invariably depend on core small non coding RNAs and that achieve dedicated sequence-specific functions. RNA silencing has been recognized to carry critical developmental, stress-response and bodyguard functions be coordinating the expression, protection, stability and inheritance of virtually all eukaryotic genomes. Thus, the ncRNAs encompass a wide set of mechanisms that achieve specialized functions.

RNA Based Technologies for Functional Genomics in Plants

RNA Based Technologies for Functional Genomics in Plants
Author: Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh
Publsiher: Springer Nature
Total Pages: 428
Release: 2021-05-13
ISBN 10: 3030649946
ISBN 13: 9783030649944
Language: EN, FR, DE, ES & NL

RNA Based Technologies for Functional Genomics in Plants Book Review:

This book offers a unique and comprehensive overview of key RNA-based technologies, as well as their development and applications for the functional genomics of plant coding and non-coding genes. It focuses on the latest as well as classical RNA-based techniques used for studies on small RNAs, long non-coding RNAs and protein-coding genes. These techniques chiefly focus on target mimics (TMs) and short tandem target mimics (STTMs) for small RNAs, and artificial microRNAs (amiRNAs), RNA interference (RNAi) and CRISPR/Cas for genes. Furthermore, the book discusses the latest trends in the field and various modifications of the above-mentioned approaches, and explores how these RNA-based technologies have been developed, applied and validated as essential technologies in plant functional genomics. RNA-based technologies, their mechanisms of action, their advantages and disadvantages, and insights into the further development and applications of these technologies in plants are discussed. These techniques will enable the users to functionally characterize genes and small RNAs through silencing, overexpression and editing. Gathering contributions by globally respected experts, the book will appeal to students, teachers and scientists in academia and industry who are interested in horticulture, genetics, pathology, entomology, physiology, molecular genetics and breeding, in vitro culture & genetic engineering, and functional genomics.

Small Non Coding RNAs

Small Non Coding RNAs
Author: Mathieu Rederstorff
Publsiher: Humana Press
Total Pages: 238
Release: 2015-03-21
ISBN 10: 9781493925469
ISBN 13: 1493925466
Language: EN, FR, DE, ES & NL

Small Non Coding RNAs Book Review:

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in the field of RNA biology and to those just starting to study small non-coding RNAs.

The Role of MicroRNAs in Plants

The Role of MicroRNAs in Plants
Author: Anthony A. Millar
Publsiher: MDPI
Total Pages: 174
Release: 2020-05-27
ISBN 10: 3039287303
ISBN 13: 9783039287307
Language: EN, FR, DE, ES & NL

The Role of MicroRNAs in Plants Book Review:

Discovered in plants at the turn of the century, microRNAs (miRNAs) have been found to be fundamental to many aspects of plant biology. These small (20–24 nt) regulatory RNAs are derived via processing from longer imperfect double-stranded RNAs. They are then incorporated into silencing complexes, which they guide to (m)RNAs of high sequence complementarity, resulting in gene silencing outcomes, either via RNA degradation and/or translational inhibition. Some miRNAs are ancient, being present in all species of land plants and controlling fundamental processes such as phase change, organ polarity, flowering, and leaf and root development. However, there are many more miRNAs that are much less conserved and with less understood functions. This Special Issue contains seven research papers that span from understanding the function of a single miRNA family to examining how the miRNA profiles alter during abiotic stress or nutrient deficiency. The possibility of circular RNAs in plants acting as miRNA decoys to inhibit miRNA function is investigated, as was the hierarchical roles of miRNA biogenesis factors in the maintenance of phosphate homeostasis. Three reviews cover the potential of miRNAs for agronomic improvement of maize, the role of miRNA-triggered secondary small RNAs in plants, and the potential function of an ancient plant miRNA.

Plant MicroRNAs

Plant MicroRNAs
Author: Blake C. Meyers,Pamela J. Green
Publsiher: Humana Press
Total Pages: 261
Release: 2012-08-09
ISBN 10: 9781617796531
ISBN 13: 1617796530
Language: EN, FR, DE, ES & NL

Plant MicroRNAs Book Review:

MicroRNAs constitute a particularly important class of small RNAs given their abundance, broad phylogenetic conservation and strong regulatory effects, with plant miRNAs uniquely divulging their ancient evolutionary origins and their strong post-transcriptional regulatory effects. In Plant MicroRNAs: Methods and Protocols, experts in the field present chapters that focus on the identification, validation, and characterization of the miRNA class of RNAs, and address important aspects about heterochromatic small interfering RNAs. In addition, the methods contained in this volume emphasize miRNA analyses, but also include ways to distinguish one class of small RNAs from another. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Plant MicroRNAs: Methods and Protocols provides the research community with a set of protocols that will help advance vital miRNA research for all plant species, both in typical model species and non-model species alike.

Plant Epigenetics

Plant Epigenetics
Author: Nikolaus Rajewsky,Stefan Jurga,Jan Barciszewski
Publsiher: Springer
Total Pages: 536
Release: 2017-04-27
ISBN 10: 3319555200
ISBN 13: 9783319555201
Language: EN, FR, DE, ES & NL

Plant Epigenetics Book Review:

This book presents, in 26 chapters, the status quo in epigenomic profiling. It discusses how functional information can be indirectly inferred and describes the new approaches that promise functional answers, collectively referred to as epigenome editing. It highlights the latest important advances in our understanding of the functions of plant epigenomics and new technologies for the study of epigenomic marks and mechanisms in plants. Topics include the deposition or removal of chromatin modifications and histone variants, the role of epigenetics in development and response to environmental signals, natural variation and ecology, as well as applications for epigenetics in crop improvement. Discussing areas ranging from the complex regulation of stress and heterosis to the precise mechanisms of DNA and histone modifications, it presents breakthroughs in our understanding of complex phenotypic phenomena.

Plant Genes Genomes and Genetics

Plant Genes  Genomes and Genetics
Author: Erich Grotewold,Joseph Chappell,Elizabeth A. Kellogg
Publsiher: John Wiley & Sons
Total Pages: 264
Release: 2015-06-02
ISBN 10: 1119998883
ISBN 13: 9781119998884
Language: EN, FR, DE, ES & NL

Plant Genes Genomes and Genetics Book Review:

Plant Genes, Genomes and Genetics provides a comprehensive treatment of all aspects of plant gene expression. Unique in explaining the subject from a plant perspective, it highlights the importance of key processes, many first discovered in plants, that impact how plants develop and interact with the environment. This text covers topics ranging from plant genome structure and the key control points in how genes are expressed, to the mechanisms by which proteins are generated and how their activities are controlled and altered by posttranslational modifications. Written by a highly respected team of specialists in plant biology with extensive experience in teaching at undergraduate and graduate level, this textbook will be invaluable for students and instructors alike. Plant Genes, Genomes and Genetics also includes: specific examples that highlight when and how plants operate differently from other organisms special sections that provide in-depth discussions of particular issues end-of-chapter problems to help students recapitulate the main concepts rich, full-colour illustrations and diagrams clearly showing important processes in plant gene expression a companion website with PowerPoint slides, downloadable figures, and answers to the questions posed in the book Aimed at upper level undergraduates and graduate students in plant biology, this text is equally suited for advanced agronomy and crop science students inclined to understand molecular aspects of organismal phenomena. It is also an invaluable starting point for professionals entering the field of plant biology.

Abiotic Stress Signaling in Plants Functional Genomic Intervention

Abiotic Stress Signaling in Plants  Functional Genomic Intervention
Author: Girdhar K. Pandey,Manoj Prasad,Amita Pandey,Maik Boehmer
Publsiher: Frontiers Media SA
Total Pages: 636
Release: 2016-08-08
ISBN 10: 288919891X
ISBN 13: 9782889198917
Language: EN, FR, DE, ES & NL

Abiotic Stress Signaling in Plants Functional Genomic Intervention Book Review:

Abiotic stresses such as high temperature, low-temperature, drought and salinity limit crop productivity worldwide. Understanding plant responses to these stresses is essential for rational engineering of crop plants. In Arabidopsis, the signal transduction pathways for abiotic stresses, light, several phytohormones and pathogenesis have been elucidated. A significant portion of plant genomes (Arabidopsis and rice were mostly studied) encodes for proteins involves in signaling such as receptor, sensors, kinases, phosphatases, transcription factors and transporters/channels. Despite decades of physiological and molecular effort, knowledge pertaining to how plants sense and transduce low and high temperature, low-water availability (drought), water-submergence, microgravity and salinity signals is still a major question for plant biologist. One major constraint hampering our understanding of these signal transduction processes in plants has been the lack or slow pace of application of molecular genomic and genetics knowledge in the form of gene function. In the post-genomic era, one of the major challenges is investigation and understanding of multiple genes and gene families regulating a particular physiological and developmental aspect of plant life cycle. One of the important physiological processes is regulation of stress response, which leads to adaptation or adjustment in response to adverse stimuli. With the holistic understanding of the signaling pathways involving not only one gene family but multiple genes or gene families, plant biologist can lay a foundation for designing and generating future crops, which can withstand the higher degree of environmental stresses (especially abiotic stresses, which are the major cause of crop loss throughout the world) without losing crop yield and productivity. Therefore, in this e-Book, we intend to incorporate the contribution from leading plant biologists to elucidate several aspects of stress signaling by functional genomics approaches.

Genome wide Analysis of Plant Heterochromatic Short interfering RNAs

Genome wide Analysis of Plant Heterochromatic Short interfering RNAs
Author: Feng Wang
Publsiher: Unknown
Total Pages: 135
Release: 2016
ISBN 10: 1928374650XXX
ISBN 13: OCLC:992906584
Language: EN, FR, DE, ES & NL

Genome wide Analysis of Plant Heterochromatic Short interfering RNAs Book Review:

Small RNAs, usually 20-24 nt in length, are critical regulators of plant transcriptomes. They are loaded into ARGONAUTE (AGO) proteins, and mediate gene silencing by interacting with target transcripts through sequence-specific base pairing. In plants, small RNAs are classified into various different groups based on their biogenesis and function; microRNAs (miRNAs) and heterochromatic short-interfering RNAs (het-siRNAs) are the two most important types of small RNAs in flowering plants. Plant small RNAs are subject to various forms of modification. Despite intensive studies about miRNA modification, knowledge about het-siRNA modification is lacking. I systematically studied non-templated nucleotide patterns in plant small RNAs by analyzing small RNA sequencing (sRNA-seq) libraries from Arabidopsis, tomato, Medicago, rice, maize and Physcomitrella. Elevated rates of non-templated nucleotides were observed at the 3' end of plant small RNAs from wild-type specimens of all analyzed species. In all species I analyzed, 'off-sized' small RNAs, such as 25 nt and 23 nt siRNAs arising from het-siRNA loci, often had higher rates of non-templated nucleotides at the 3' end. In Arabidopsis, 23 nt siRNAs arising from het-siRNA clusters display a distinct pattern of 3'-non-templated nucleotides. This pattern of 3'-non-templated nucleotides in 23 nt siRNAs is not dependent on known terminal nucleotidyl transferases, and may result from modifications added to longer het-siRNA precursors.Het-siRNAs negatively regulate gene expression through the RNA-directed DNA methylation (RdDM) pathway. Biogenesis of most het-siRNAs depends on the plant-specific RNA polymerase IV (Pol IV), and AGO4 is the major effector protein of het-siRNAs. Through genome-wide analysis of sRNA-seq data sets, I found that AGO4 is required for the accumulation of a small subset of het-siRNAs in Arabidopsis thaliana. The accumulation of AGO4-dependent het-siRNAs also requires several other RdDM components, including RNA POLYMERASE V (Pol V), DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2) and SAWADEE HOMEODOMAIN HOMOLOG 1 (SHH1). I also demonstrated that het-siRNA accumulation could not be fully recovered by a slicing-defective AGO4 from ago4 mutant plants. These data suggest that AGO4-dependent het-siRNAs are secondary het-siRNAs, whose biogenesis requires prior activities of RdDM at certain loci.In the current RdDM model, AGO4-bound het-siRNAs target Pol V transcripts through sequence-specific base pairing. However, the details of such interactions are largely unknown. Through crosslinking immunoprecipitation by an Arabidopsis AGO4 antibody and subsequent high-throughput sequencing, I identified a handful of het-siRNA:target interactions in Arabidopsis thaliana. These de novo identified het-siRNA:target interactions suggest that het-siRNAs act on both cis and trans loci. Successful interaction between a het-siRNA and its target(s) requires extensive base-pairing, and induces target cleavage between the 10th to 11th nucleotide counting from the 5' end of het-siRNAs.

Plant Gene Silencing

Plant Gene Silencing
Author: Tamas Dalmay
Publsiher: CABI
Total Pages: 223
Release: 2017-05-29
ISBN 10: 1780647670
ISBN 13: 9781780647678
Language: EN, FR, DE, ES & NL

Plant Gene Silencing Book Review:

Plant gene silencing is a crucially important phenomenon in gene expression and epigenetics. This book describes the way small RNA is produced and acts to silence genes, its likely origins in defence against viruses, and also its potential to improve plants. Plant gene silencing can be used to improve industrial traits, make plants more nutritious or more valuable to consumers, to remove allergens, and to improve resistance to weeds and pathogens.

MiRNA Biogenesis

MiRNA Biogenesis
Author: Ulf Andersson Ørom
Publsiher: Unknown
Total Pages: 238
Release: 2018
ISBN 10: 9781493986248
ISBN 13: 1493986244
Language: EN, FR, DE, ES & NL

MiRNA Biogenesis Book Review:

RNA Abundance Analysis

RNA Abundance Analysis
Author: Hailing Jin,Walter Gassmann
Publsiher: Humana Press
Total Pages: 248
Release: 2016-05-01
ISBN 10: 9781493959167
ISBN 13: 1493959166
Language: EN, FR, DE, ES & NL

RNA Abundance Analysis Book Review:

This book examines a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches.

Phytonutritional Improvement of Crops

Phytonutritional Improvement of Crops
Author: Noureddine Benkeblia
Publsiher: John Wiley & Sons
Total Pages: 544
Release: 2017-10-02
ISBN 10: 1119079942
ISBN 13: 9781119079941
Language: EN, FR, DE, ES & NL

Phytonutritional Improvement of Crops Book Review:

Chapter 13: Development of the Transgenic Rice Accumulating Flavonoids in Seed by Metabolic Engineering -- 13.1 Introduction -- 13.2 Production of Flavonoids in Rice Seed by Ectopic Expression of the Biosynthetic Enzymes -- 13.3 Production of Flavonoids in Rice Seed by Ectopic Expression of the Transcription Factors -- 13.4 Characterisation of Flavonoids in Transgenic Rice Seed by LC-MS-based Metabolomics -- 13.5 Future Prospects -- References -- Chapter 14: Nutrient Management for High Efficiency Sweetpotato Production -- 14.1 Patterns of Growth and Development and Nutrient Absorption in Sweetpotato -- 14.2 Screening of High Efficient of Potassium Uptake and Utilised Genotypes -- 14.3 Effect of Fertilisers -- 14.4 Balanced Fertiliser Management in Sweetpotato at Sishui, Shandong: A Case Study -- 14.5 Application of Fertilisers Through Drip Irrigation ('Fertigation') -- Acknowledgements -- References -- Index -- End User License Agreement