Western Blotting Guru

PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocols Contains unique "Good Practices and Tips” that are indispensable for the beginner and expert alike Features "Special Cases” with applications of PCR, optimization, and troubleshooting Includes detailed appendices with tables, figures, and key protocols Organized as a systematic, concentrated resource to save time when addressing a PCR problem

Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types. Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments Contains unique good practices and tips that are indispensable for the beginner and expert alike Features special cases with applications of immunoblotting optimization Includes detailed appendices with tables, figures and key protocols Provides troubleshooting tips for various types of modifications of standard protocols Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem

Since the first volume was published, there has been significant success in isolating genes responsible for particular cancers as well as a major improvement in our understanding of the molecular events leading to tumors. This book explores possible genetic treatments that can suppress cancer cells that have formed tumors and it presents the details of the isolation and characterization of new human cancer genes that have recently been identified. Molecular Genetics of Cancer, 2E is an essential book for anyone involved in cancer research and the search for a cure.

Molecular Genetics of Cancer, Second Edition provides an authoritative and up to date review of the key genes known to be critical in the development or progression of cancer. Throughout the book, scientific advances and their clinical relevance are covered in detail, particularly in the light of findings concerning the inheritance of genes predisposing to tumorigenesis. The book is therefore a valuable source of reference for clinicians and genetic counsellors as well as researchers.

From the author of White Tears, this bestselling, award-winning novel of a young man’s journey explores what it means to be Indian or English, black or white, and every degree that lies between. Pran Nath Razdan, the boy who will become the Impressionist, was passed off by his Indian mother as the child of her husband, a wealthy man of a high caste. Pran lived a life of luxury just downriver from the Taj Mahal, but at fifteen, the news of Pran’s true parentage is revealed to his father and he is tossed out into the street—a pariah and an outcast. Thus begins an extraordinary, near mythical journey of a young man who must reinvent himself to survive—not once, but many times. From Victorian India to Edwardian London, from an expatriate community of black Americans in Paris to a hopeless expedition to study a lost tribe of Africa, Hari Kunzru’s unforgettable debut novel dazzles with its artistry and wit while it challenges with its insights into the self, nationality, race, and beyond.

Explores Jung's psychological concepts regarding the nature, function and importance of man's symbols as they appear on both the conscious and subconscious level

This is the story of LSD told by a concerned yet hopeful father, organic chemist Albert Hofmann. He traces LSDs path from a promising psychiatric research medicine to a recreational drug sparking hysteria and prohibition.We follow Dr. Hofmanns trek across Mexico to discover sacred plants related to LSD, and listen in as he corresponds with other notable figures about his remarkable discovery.Underlying it all is Dr. Hofmanns powerful conclusion that mystical experience may be our planets best hope for survival. Whether induced by LSD, meditation, or arising spontaneously, such experiences help us to comprehend the wonder, the mystery of the divine in the microcosm of the atom, in the macrocosm of the spiral nebula, in the seeds of plants, in the body and soul of people.Now, more than sixty years after the birth of Albert Hofmanns problem child, his vision of its true potential is more relevant, and more needed, than ever.

Antibodies—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Monoclonal Antibodies. The editors have built Antibodies—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Monoclonal Antibodies in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Antibodies—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

At the close of the last millennium, Helen Fielding debuted the irrepressible (and blockbuster-bestselling) Bridget Jones. Now, Fielding gives us a sensational new heroine for a new era...Move over 007, a stunning, sexy-and decidedly female-new player has entered the world of international espionage. Her name is Olivia Joules (that’s "J.O.U.L.E.S. the unit of kinetic energy") and she's ready to take America by storm with charm, style, and her infamous Overactive Imagination. How could a girl not be drawn to the alluring, powerful Pierre Ferramo-he of the hooded eyes, impeccable taste, unimaginable wealth, exotic international homes, and dubious French accent? Could Ferramo really be a major terrorist bent on the Western world’s destruction, hiding behind a smokescreen of fine wines, yachts, and actresses slash models? Or is it all just a product of Olivia Joules’s overactive imagination? Join Olivia in her heart-stopping, hilarious, nerve-frazzling quest from hip hotel to eco-lodge to underwater cave, by light aircraft, speedboat, helicopter, and horse, in this witty, contemporary, and utterly unputdownable novel deluxe.

In California the hills are on fire. Not a good sign for Andrew Kindler, who just came from back east to get away from his past–as an arsonist. In fact, almost from the moment he sets foot in his cousin’s Santa Monica beach house, the heat starts swirling around him. First there’s the cop who thinks Andrew might know something about a murder suspect. Then there’s the suspect’s beautiful sister, who is willing to pay Andrew $5,000 for the same information. But Andrew really uninformed. And with a sensational murder case burning a hole in the gut of the LAPD–as well as the star-studded L.A. fitness industry–ignorance is dangerous. Now Andrew must solve a murder he knows nothing about, find a killer he’s never met, and unravel a family’s explosive secret. His reward for success? To live another day: one step ahead of his burning past... “An exceptionally well-crafted and well-told tale of arson, police work, misplaced zeal, bad relationships, good relationships, family bonds and, oh yes, exercise videos. Quirky, compelling, intelligent, and funny ... If you like Elmore Leonard, do yourself a favor and pick up BURN.”–Lincoln Journal Star “A cult writer for the masses–hip, smart and so mordantly funny that the casual reader might be laughing too hard to realize just how thoughtful Doolittle’s work is. Get on the bandwagon now.”–Laura Lippman, author of By a Spider’s Thread “Sean Doolittle combines wit, good humor, and a generosity of spirit rare in mystery fiction to create novels that are both engrossing and strangely uplifting. He deserves to take his place among the best in the genre.”–John Connolly, author of The White Road “An estimable addition not only to the publisher’s list but also to crime fiction ... Doolittle delivers a briskly plotted, hard-boiled mystery that has its roots in the Elmore Leonard school of dark comedy.”–South Florida Sun-Sentinel ·Gold medal winner for mystery in ForeWord Magazine’s Book of the Year Award ·A Best Crime Fiction of 2003 pick from January Magazine

Ken Wilber's latest book is a daring departure from his previous writings—a highly original work of fiction that combines brilliant scholarship with tongue-in-cheek storytelling to present the integral approach to human development that he expounded in more conventional terms in his recent A Theory of Everything. The story of a naïve young grad student in computer science and his quest for meaning in a fragmented world provides the setting in which Wilber contrasts the alienated "flatland" of scientific materialism with the integral vision, which embraces body, mind, soul, and spirit in self, culture, and nature. The book especially targets one of the most stubborn obstacles to realizing the integral vision: a disease of egocentrism and narcissism that Wilber calls "boomeritis" because it seems to plague the baby-boomer generation most of all. Through a series of sparkling seminar-lectures skillfully interwoven with the hero's misadventures in the realms of sex, drugs, and popular culture, all of the major tenets of extreme postmodernism are criticized—and exemplified—including the author's having a bad case of boomeritis himself. Parody, intellectual slapstick, and a mind-twisting surprise ending unite to produce a highly entertaining summary of the work of cutting-edge theorists in human development from around the world.

America and Britain swirl together in this delightful satire. Dennis Barlow came to America as a poet, hired to write a screenplay for a movie studio. But that ended badly, and so he has taken a job at a pet cemetery, a choice that has left other members of the British expat community in Hollywood quite taken aback, though they are quickly distracted by the suicide of one of their own. Barlow is tasked with the funeral arrangements, during which process he meets the beautiful Aimée Thanatogenos. Can he keep himself in the good graces of his countrymen, and Ms. Thanatogenos – especially after the passing of other poets’ works as his own? Penguin Random House Canada is proud to bring you classic works of literature in e-book form, with the highest quality production values. Find more today and rediscover books you never knew you loved.

The AACR Annual Meeting highlights the best cancer science and medicine from institutions all over the world. Attendees are invited to stretch their boundaries, form collaborations, attend sessions outside their own areas of expertise, and learn how to apply exciting new concepts, tools, and techniques to their own research. Part A contains abstracts 1-3062 accepted for the 2017 meeting.

“Dunhuang Manuscript Culture” explores the world of Chinese manuscripts from ninth-tenth century Dunhuang, an oasis city along the network of pre-modern routes known today collectively as the Silk Roads. The manuscripts have been discovered in 1900 in a sealed-off side-chamber of a Buddhist cave temple, where they had lain undisturbed for for almost nine hundred years. The discovery comprised tens of thousands of texts, written in over twenty different languages and scripts, including Chinese, Tibetan, Old Uighur, Khotanese, Sogdian and Sanskrit. This study centres around four groups of manuscripts from the mid-ninth to the late tenth centuries, a period when the region was an independent kingdom ruled by local families. The central argument is that the manuscripts attest to the unique cultural diversity of the region during this period, exhibiting—alongside obvious Chinese elements—the heavy influence of Central Asian cultures. As a result, it was much less ‘Chinese’ than commonly portrayed in modern scholarship. The book makes a contribution to the study of cultural and linguistic interaction along the Silk Roads.

"Fundamentals of Tissue Engineering and Regenerative Medicine" provides a complete overview of the state of the art in tissue engineering and regenerative medicine. Tissue engineering has grown tremendously during the past decade. Advances in genetic medicine and stem cell technology have significantly improved the potential to influence cell and tissue performance, and have recently expanded the field towards regenerative medicine. In recent years a number of approaches have been used routinely in daily clinical practice, others have been introduced in clinical studies, and multitudes are in the preclinical testing phase. Because of these developments, there is a need to provide comprehensive and detailed information for researchers and clinicians on this rapidly expanding field. This book offers, in a single volume, the prerequisites of a comprehensive understanding of tissue engineering and regenerative medicine. The book is conceptualized according to a didactic approach (general aspects: social, economic, and ethical considerations; basic biological aspects of regenerative medicine: stem cell medicine, biomolecules, genetic engineering; classic methods of tissue engineering: cell, tissue, organ culture; biotechnological issues: scaffolds; bioreactors, laboratory work; and an extended medical discipline oriented approach: review of clinical use in the various medical specialties). The content of the book, written in 68 chapters by the world’s leading research and clinical specialists in their discipline, represents therefore the recent intellect, experience, and state of this bio-medical field.

This book offers a collection of information on successive steps of molecular ‘dialogue’ between plants and pathogens. It additionally presents data that reflects intrinsic logic of plant-parasite interactions. New findings discussed include: host and non-host resistance, specific and nonspecific elicitors, elicitors and suppressors, and plant and animal immunity. This book enables the reader to understand how to promote or prevent disease development, and allows them to systematize their own ideas of plant-pathogen interactions. * Offers a more extensive scope of the problem as compared to other books in the market * Presents data to allow consideration of host-parasite relationships in dynamics and reveals interrelations between pathogenicity and resistance factors * Discusses beneficial plant-microbe interactions and practical aspects of molecular investigations of plant-parasite relationships * Compares historical study of common and specific features of plant immunity with animal immunity

The fourth of the five Richard Hannay novels by John Buchan. Here we find our hero Richard Hannay living a quiet life in the countryside with a wife and young child but his past comes back to haunt him and he once more must face up to an arch-enemy.

Presents the further adventures of Mowgli, a boy reared by a pack of wolves, and the wild animals of the jungle. Also includes other short stories set in India.

The CCN Proteins are thought to play key roles in the biology of normal cell, tissue, organ, and body, and altered expression of CCN proteins is associated with several pathologies, including fibrosis and cancer. Because of its importance, the CCN field is expanding at a fast pace. Research articles in this field have recently increased logarithmically, and a book that is up-to-date, comprehensive, authoritative and affords insights into the biological roles of CCN proteins, is timely. CCN Protein: A New Family of Cell Growth and Differentiation Regulators presents the most recent progress in the field of CCN proteins, a new family of secretory signaling molecules that are involved in several fundamental biological progress. These proteins share a unique multimodular organization and present a partial identity with four families of regulatory proteins controling growth and development. The book covers the roles of CCN proteins in the control of cell proliferation and differentiation during normal development, wound repair, chondrogenesis and bone development, angiogenesis, tissue regeneration, fibrosis, renal diseases and cancer development. Contents: The CCN Family of Proteins: An Overview (B Perbal & M Takigawa)Roles of CCN2/CTGF in the Control of Growth and Regeneration (M Takigawa et al.)Integrin-Mediated CCN Functions (L F Lau & S C-T Lam)Expression and Roles of CCN2 During Odontogenesis (M Kanyama et al.)CCN Genes and the Kidney (B L Riser et al.)CCN Proteins in Liver Injury and Disease (A W Rachfal & D R Brigstock)Genetic Analysis of CCN Gene Function in Mammalian Development (L M Dornbach & K M Lyons)CCN Family in Embryonic Development (Non-Mammalian Models) (B Latinkic)CCN3 Expression and its Role During Development (K-I Katsube)Regulation of CCN Proteins by Alternations of the Cytoskeleton (B Chaqour & M Goppelt-Struebe)Pathogenesis of Systemic Sclerosis and CCN2 (Connective Tissue Growth Factor) (K Takehara)Function and Regulation of CCN5 (M R Gray & J J Castellot Jr)CCN3: A Multifunctional Signaling Regulator (N Planque et al.)CCN Proteins and Connexins: Interactions and Growth Control (C Fu et al.)The Role of CCN1 in Tumorigenesis and Cancer Progression (J O'Kelly & H P Koeffler)CCN4 and CCN6 Variants in Wnt-Inducible Signaling Pathway (S Tanaka) Readership: Biochemists, biologists, molecular biologists, physiologists, pathologists, and medical and dental doctors interested in the study of CCN proteins, growth factors, extracellular matrix proteins, signaling molecules, and mechanisms of gene expression. Key Features:First such book in the CCN fieldChapters are written by the most prominent specialists in the fieldKeywords:Cyr 61;CTGF;NOV;WISP;Elm-1;rCop-1;Growth Factor;Extracellular Matrix;Signaling;Gene Expression;Cancer Fibrogenesis