Next Generation Sequencing

Next Generation Sequencing
Author: Jerzy Kulski
Publsiher: BoD – Books on Demand
Total Pages: 464
Release: 2016-01-14
ISBN 10: 9535122401
ISBN 13: 9789535122401
Language: EN, FR, DE, ES & NL

Next Generation Sequencing Book Review:

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Next Generation Genome Sequencing

Next Generation Genome Sequencing
Author: Michal Janitz
Publsiher: John Wiley & Sons
Total Pages: 282
Release: 2011-08-24
ISBN 10: 3527644733
ISBN 13: 9783527644735
Language: EN, FR, DE, ES & NL

Next Generation Genome Sequencing Book Review:

Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.

Next Generation Sequencing

Next Generation Sequencing
Author: Steven R. Head,Phillip Ordoukhanian,Daniel R. Salomon
Publsiher: Humana Press
Total Pages: 266
Release: 2017-12-10
ISBN 10: 9781493975129
ISBN 13: 1493975129
Language: EN, FR, DE, ES & NL

Next Generation Sequencing Book Review:

This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications, method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field.

Clinical Applications for Next Generation Sequencing

Clinical Applications for Next Generation Sequencing
Author: Urszula Demkow,Rafal Ploski
Publsiher: Academic Press
Total Pages: 334
Release: 2015-09-10
ISBN 10: 0128018410
ISBN 13: 9780128018415
Language: EN, FR, DE, ES & NL

Clinical Applications for Next Generation Sequencing Book Review:

Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. Fills the gap between state-of-the-art technology and evidence-based practice Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment Promotes a practical tool that clinicians can apply directly to patient care Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases Presents evidence regarding the important role of NGS in current diagnostic strategies

Next Generation Sequencing Data Analysis

Next Generation Sequencing Data Analysis
Author: Xinkun Wang
Publsiher: CRC Press
Total Pages: 258
Release: 2016-04-06
ISBN 10: 1482217899
ISBN 13: 9781482217896
Language: EN, FR, DE, ES & NL

Next Generation Sequencing Data Analysis Book Review:

A Practical Guide to the Highly Dynamic Area of Massively Parallel SequencingThe development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphi

Bioinformatics A Practical Handbook Of Next Generation Sequencing And Its Applications

Bioinformatics  A Practical Handbook Of Next Generation Sequencing And Its Applications
Author: Low Lloyd Wai Yee,Tammi Martti Tapani
Publsiher: #N/A
Total Pages: 252
Release: 2017-07-26
ISBN 10: 9813144769
ISBN 13: 9789813144767
Language: EN, FR, DE, ES & NL

Bioinformatics A Practical Handbook Of Next Generation Sequencing And Its Applications Book Review:

Rapid technological developments have led to increasingly efficient sequencing approaches. Next Generation Sequencing (NGS) is increasingly common and has become cost-effective, generating an explosion of sequenced data that need to be analyzed. The skills required to apply computational analysis to target research on a wide range of applications that include identifying causes of cancer, vaccine design, new antibiotics, drug development, personalized medicine and higher crop yields in agriculture are highly sought after. This invaluable book provides step-by-step guides to complex topics that make it easy for readers to perform essential analyses from raw sequenced data to answering important biological questions. It is an excellent hands-on material for teachers who conduct courses in bioinformatics and as a reference material for professionals. The chapters are written to be standalone recipes making it suitable for readers who wish to self-learn selected topics. Readers will gain skills necessary to work on sequenced data from NGS platforms and hence making themselves more attractive to employers who need skilled bioinformaticians to handle the deluge of data.

Next Generation Sequencing in Forensic Science

Next Generation Sequencing in Forensic Science
Author: Kelly M. Elkins,Cynthia B. Zeller
Publsiher: CRC Press
Total Pages: 192
Release: 2021-09-16
ISBN 10: 1000449254
ISBN 13: 9781000449259
Language: EN, FR, DE, ES & NL

Next Generation Sequencing in Forensic Science Book Review:

Next Generation Sequencing in Forensic Science: A Primer addresses next generation sequencing (NGS) specific to its application to forensic science. The first part of the book offers a history of human identity approaches, including VNTR, RFLP, STR, and SNP DNA typing. It discusses the history of sequencing for human DNA typing, including Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. The chapters present an overview of the forensically focused AmpliSeq, ForenSeq, Precision ID, PowerSeq, and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs using the MiSeq FGx and Ion Torrent System. The authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef, including creating a sample list, executing wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained, including how to analyze and interpret NGS data and output graphs and charts. The book concludes with mitochondrial DNA (mtDNA) sequencing and SNPs analysis, including the issue of heteroplasmy. The final chapters review forensic applications of microbial DNA, NGS in body fluid analysis, and challenges and considerations for future applications. FEATURES Focuses on human identification using traditional and NGS DNA typing methods targeting short tandem repeats (STRs) Applies the technology and its application to law enforcement investigations and identity and ancestry single nucleotide polymorphisms (SNPs) for investigational leads, mass disaster, and ancestry cases Presents the underlying principles of NGS in a clear, easy-to-understand format for practitioners and students studying DNA in forensic programs This is the first book to prepare practitioners to utilize and implement this new technology in their lab for casework, highlighting early applications of how NGS results have been used in court. The book can be utilized for upper-level undergraduate and graduate students taking courses focused on NGS concepts. Readers are expected to have a basic understanding of molecular and cellular biology and DNA typing.

Tag based Next Generation Sequencing

Tag based Next Generation Sequencing
Author: Guenter Kahl
Publsiher: John Wiley & Sons
Total Pages: 581
Release: 2012-02-13
ISBN 10: 352732819X
ISBN 13: 9783527328192
Language: EN, FR, DE, ES & NL

Tag based Next Generation Sequencing Book Review:

Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be performed by tag-based approaches, where the focus shifts from 'sequencing power' to better means of data analysis and visualization for common users. Today Next Generation Sequence data require powerful bioinformatics expertise that has to be converted into easy-to-use data analysis tools. The book's intention is to give an overview on recently developed tag-based approaches along with means of their data analysis together with introductions to Next-Generation Sequencing Methods, protocols and user guides to be an entry for scientists to tag-based approaches for Next Generation Sequencing.

Next Generation Sequencing Technologies and Challenges in Sequence Assembly

Next Generation Sequencing Technologies and Challenges in Sequence Assembly
Author: Sara El-Metwally,Osama M. Ouda,Mohamed Helmy
Publsiher: Springer Science & Business
Total Pages: 118
Release: 2014-04-19
ISBN 10: 1493907158
ISBN 13: 9781493907151
Language: EN, FR, DE, ES & NL

Next Generation Sequencing Technologies and Challenges in Sequence Assembly Book Review:

The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Algorithms for Next Generation Sequencing

Algorithms for Next Generation Sequencing
Author: Wing-Kin Sung
Publsiher: CRC Press
Total Pages: 350
Release: 2017-05-18
ISBN 10: 1466565519
ISBN 13: 9781466565517
Language: EN, FR, DE, ES & NL

Algorithms for Next Generation Sequencing Book Review:

Advances in sequencing technology have allowed scientists to study the human genome in greater depth and on a larger scale than ever before – as many as hundreds of millions of short reads in the course of a few days. But what are the best ways to deal with this flood of data? Algorithms for Next-Generation Sequencing is an invaluable tool for students and researchers in bioinformatics and computational biology, biologists seeking to process and manage the data generated by next-generation sequencing, and as a textbook or a self-study resource. In addition to offering an in-depth description of the algorithms for processing sequencing data, it also presents useful case studies describing the applications of this technology.

Computational Methods for Next Generation Sequencing Data Analysis

Computational Methods for Next Generation Sequencing Data Analysis
Author: Ion Mandoiu,Alexander Zelikovsky
Publsiher: John Wiley & Sons
Total Pages: 464
Release: 2016-09-12
ISBN 10: 1119272173
ISBN 13: 9781119272175
Language: EN, FR, DE, ES & NL

Computational Methods for Next Generation Sequencing Data Analysis Book Review:

Introduces readers to core algorithmic techniques for next-generation sequencing (NGS) data analysis and discusses a wide range of computational techniques and applications This book provides an in-depth survey of some of the recent developments in NGS and discusses mathematical and computational challenges in various application areas of NGS technologies. The 18 chapters featured in this book have been authored by bioinformatics experts and represent the latest work in leading labs actively contributing to the fast-growing field of NGS. The book is divided into four parts: Part I focuses on computing and experimental infrastructure for NGS analysis, including chapters on cloud computing, modular pipelines for metabolic pathway reconstruction, pooling strategies for massive viral sequencing, and high-fidelity sequencing protocols. Part II concentrates on analysis of DNA sequencing data, covering the classic scaffolding problem, detection of genomic variants, including insertions and deletions, and analysis of DNA methylation sequencing data. Part III is devoted to analysis of RNA-seq data. This part discusses algorithms and compares software tools for transcriptome assembly along with methods for detection of alternative splicing and tools for transcriptome quantification and differential expression analysis. Part IV explores computational tools for NGS applications in microbiomics, including a discussion on error correction of NGS reads from viral populations, methods for viral quasispecies reconstruction, and a survey of state-of-the-art methods and future trends in microbiome analysis. Computational Methods for Next Generation Sequencing Data Analysis: Reviews computational techniques such as new combinatorial optimization methods, data structures, high performance computing, machine learning, and inference algorithms Discusses the mathematical and computational challenges in NGS technologies Covers NGS error correction, de novo genome transcriptome assembly, variant detection from NGS reads, and more This text is a reference for biomedical professionals interested in expanding their knowledge of computational techniques for NGS data analysis. The book is also useful for graduate and post-graduate students in bioinformatics.

Next Generation Sequencing

Next Generation Sequencing
Author: Lee-Jun C. Wong
Publsiher: Springer Science & Business Media
Total Pages: 302
Release: 2013-05-31
ISBN 10: 1461470013
ISBN 13: 9781461470014
Language: EN, FR, DE, ES & NL

Next Generation Sequencing Book Review:

In recent years, owing to the fast development of a variety of sequencing technologies in the post human genome project era, sequencing analysis of a group of target genes, entire protein coding regions of the human genome, and the whole human genome has become a reality. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) technologies offers a way to screen for mutations in many different genes in a cost and time efficient manner by deep coverage of the target sequences. This novel technology has now been applied to clinical diagnosis of Mendelian disorders of well characterized or undefined diseases, discovery of new disease genes, noninvasive prenatal diagnosis using maternal blood, and population based carrier testing of severe autosomal recessive disorders. This book covers topics of these applications, including potential limitations and expanded application in the future. ​

Next Generation Sequencing and Sequence Data Analysis

Next Generation Sequencing and Sequence Data Analysis
Author: Kuo Ping Chiu
Publsiher: Bentham Science Publishers
Total Pages: 170
Release: 2015-11-04
ISBN 10: 1681080923
ISBN 13: 9781681080925
Language: EN, FR, DE, ES & NL

Next Generation Sequencing and Sequence Data Analysis Book Review:

Nucleic acid sequencing techniques have enabled researchers to determine the exact order of base pairs - and by extension, the information present - in the genome of living organisms. Consequently, our understanding of this information and its link to genetic expression at molecular and cellular levels has lead to rapid advances in biology, genetics, biotechnology and medicine. Next-Generation Sequencing and Sequence Data Analysis is a brief primer on DNA sequencing techniques and methods used to analyze sequence data. Readers will learn about recent concepts and methods in genomics such as sequence library preparation, cluster generation for PCR technologies, PED sequencing, genome assembly, exome sequencing, transcriptomics and more. This book serves as a textbook for students undertaking courses in bioinformatics and laboratory methods in applied biology. General readers interested in learning about DNA sequencing techniques may also benefit from the simple format of information presented in the book.

Game Changer Next Generation Sequencing and Its Impact on Food Microbiology

Game Changer Next Generation Sequencing and Its Impact on Food Microbiology
Author: Jennifer Ronholm,Sabah Bidawid,Sandra Torriani
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2018-04-26
ISBN 10: 2889454630
ISBN 13: 9782889454631
Language: EN, FR, DE, ES & NL

Game Changer Next Generation Sequencing and Its Impact on Food Microbiology Book Review:

Advances in next-generation sequencing technologies (NGS) are revolutionizing the field of food microbiology. Microbial whole genome sequencing (WGS) can provide identification, characterization, and subtyping of pathogens for epidemiological investigations at a level of precision previously not possible. This allows for connections and source attribution to be inferred between related isolates that may be overlooked by traditional techniques. The archiving and global sharing of genome sequences allow for retrospective analysis of virulence genes, antimicrobial resistance markers, mobile genetic elements and other novel genes. The advent of high-throughput 16S rRNA amplicon sequencing, in combination with the advantages offered by massively parallel second-generation sequencing for metagenomics, enable intensive studies on the microbiomes of food products and the impact of foods on the human microbiome. These studies may one day lead to the development of reliable culture-independent methods for food monitoring and surveillance. Similarly, RNA-seq has provided insights into the transcriptomes and hence the behaviour of bacterial pathogens in food, food processing environments, and in interaction with the host at a resolution previously not achieved through the use of microarrays and/or RT-PCR. The vast un-tapped potential applications of NGS along with its rapidly declining costs, give this technology the ability to contribute significantly to consumer protection, global trade facilitation, and increased food safety and security. Despite the rapid advances, challenges remain. How will NGS data be incorporated into our existing global food safety infrastructure? How will massive NGS data be stored and shared globally? What bioinformatics solutions will be used to analyse and optimise these large data sets? This Research Topic discusses recent advances in the field of food microbiology made possible through the use of NGS.

Statistical Analysis of Next Generation Sequencing Data

Statistical Analysis of Next Generation Sequencing Data
Author: Somnath Datta,Dan Nettleton
Publsiher: Springer
Total Pages: 432
Release: 2014-07-03
ISBN 10: 3319072129
ISBN 13: 9783319072128
Language: EN, FR, DE, ES & NL

Statistical Analysis of Next Generation Sequencing Data Book Review:

Next Generation Sequencing (NGS) is the latest high throughput technology to revolutionize genomic research. NGS generates massive genomic datasets that play a key role in the big data phenomenon that surrounds us today. To extract signals from high-dimensional NGS data and make valid statistical inferences and predictions, novel data analytic and statistical techniques are needed. This book contains 20 chapters written by prominent statisticians working with NGS data. The topics range from basic preprocessing and analysis with NGS data to more complex genomic applications such as copy number variation and isoform expression detection. Research statisticians who want to learn about this growing and exciting area will find this book useful. In addition, many chapters from this book could be included in graduate-level classes in statistical bioinformatics for training future biostatisticians who will be expected to deal with genomic data in basic biomedical research, genomic clinical trials and personalized medicine. About the editors: Somnath Datta is Professor and Vice Chair of Bioinformatics and Biostatistics at the University of Louisville. He is Fellow of the American Statistical Association, Fellow of the Institute of Mathematical Statistics and Elected Member of the International Statistical Institute. He has contributed to numerous research areas in Statistics, Biostatistics and Bioinformatics. Dan Nettleton is Professor and Laurence H. Baker Endowed Chair of Biological Statistics in the Department of Statistics at Iowa State University. He is Fellow of the American Statistical Association and has published research on a variety of topics in statistics, biology and bioinformatics.

Challenges and Opportunities of Next Generation Sequencing for Biomedical Research

Challenges and Opportunities of Next Generation Sequencing for Biomedical Research
Author: Anonim
Publsiher: Academic Press
Total Pages: 208
Release: 2012-10-04
ISBN 10: 0123982731
ISBN 13: 9780123982735
Language: EN, FR, DE, ES & NL

Challenges and Opportunities of Next Generation Sequencing for Biomedical Research Book Review:

Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology serial has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. This volume features articles on Challenges and Opportunities of Next-Generation Sequencing for Biomedical Research. Covers reviews of methodology and research in all aspects of protein chemistry Brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics

Regnum Vegetabile Next Generation Sequencing in Plant Systematics

Regnum Vegetabile Next Generation Sequencing in Plant Systematics
Author: Eva Hörandl,Marc Appelhaus
Publsiher: Unknown
Total Pages: 294
Release: 2015
ISBN 10: 9783874294928
ISBN 13: 3874294927
Language: EN, FR, DE, ES & NL

Regnum Vegetabile Next Generation Sequencing in Plant Systematics Book Review:

Next Generation DNA Sequencing Informatics Second Edition

Next Generation DNA Sequencing Informatics  Second Edition
Author: Stuart M. Brown
Publsiher: Unknown
Total Pages: 350
Release: 2015-06-30
ISBN 10: 9781621821236
ISBN 13: 1621821234
Language: EN, FR, DE, ES & NL

Next Generation DNA Sequencing Informatics Second Edition Book Review:

"Next-generation DNA sequencing (NGS) technology has revolutionized biomedical research, making complete genome sequencing an affordable and frequently used tool for a wide variety of research applications. This book provides a thorough introduction to the necessary informatics methods and tools for operating NGS instruments and analyzing NGS data"--

Next Generation Sequencing and Sequence Assembly

Next Generation Sequencing and Sequence Assembly
Author: Ali Masoudi-Nejad,Zahra Narimani,Nazanin Hosseinkhan
Publsiher: Springer Science & Business Media
Total Pages: 86
Release: 2013-07-09
ISBN 10: 1461477263
ISBN 13: 9781461477266
Language: EN, FR, DE, ES & NL

Next Generation Sequencing and Sequence Assembly Book Review:

The goal of this book is to introduce the biological and technical aspects of next generation sequencing methods, as well as algorithms to assemble these sequences into whole genomes. The book is organized into two parts; part 1 introduces NGS methods and part 2 reviews assembly algorithms and gives a good insight to these methods for readers new to the field. Gathering information, about sequencing and assembly methods together, helps both biologists and computer scientists to get a clear idea about the field. Chapters will include information about new sequencing technologies such as ChIp-seq, ChIp-chip, and De Novo sequence assembly. ​

Next Generation Sequencing and Molecular Diagnostics

Next Generation Sequencing and Molecular Diagnostics
Author: Dimitrios H. Roukos
Publsiher: Unknown
Total Pages: 135
Release: 2013-02-01
ISBN 10: 9781780841885
ISBN 13: 1780841884
Language: EN, FR, DE, ES & NL

Next Generation Sequencing and Molecular Diagnostics Book Review: