Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry
Author: Bindesh Shrestha
Publsiher: Elsevier
Total Pages: 270
Release: 2021-05-07
ISBN 10: 0128189991
ISBN 13: 9780128189993
Language: EN, FR, DE, ES & NL

Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry Book Review:

Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. Provides the introductory foundations of imaging mass spectrometry for those new to the technique Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work Includes case studies summarizing the imaging MS techniques developed for the class of molecules

Methodological Advances of MALDI Mass Spectrometry Based Techniques in Organic and Biomedical Analysis

Methodological Advances of MALDI Mass Spectrometry Based Techniques in Organic and Biomedical Analysis
Author: Cheng Guo,Gerard Bolbach,Lucrece Matheron,Kezhi Jiang,Lei Yue,Lin Wang
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2022-07-13
ISBN 10: 2889764362
ISBN 13: 9782889764365
Language: EN, FR, DE, ES & NL

Methodological Advances of MALDI Mass Spectrometry Based Techniques in Organic and Biomedical Analysis Book Review:

Biocomputing 2007

Biocomputing 2007
Author: Anonim
Publsiher: World Scientific
Total Pages: 507
Release: 2006-12-11
ISBN 10: 9812704175
ISBN 13: 9789812704177
Language: EN, FR, DE, ES & NL

Biocomputing 2007 Book Review:

The Pacific Symposium on Biocomputing (PSB) 2007 is an international, multidisciplinary conference for the presentation and discussion of current research in the theory and application of computational methods in problems of biological significance. Presentations are rigorously peer reviewed and are published in an archival proceedings volume. PSB 2007 will be held January 3-7, 2007 at the Grand Wailea, Maui. Tutorials will be offered prior to the start of the conference.PSB 2007 will bring together top researchers from the US, the Asian Pacific nations, and around the world to exchange research results and address open issues in all aspects of computational biology. It is a forum for the presentation of work in databases, algorithms, interfaces, visualization, modeling, and other computational methods, as applied to biological problems, with emphasis on applications in data-rich areas of molecular biology.The PSB has been designed to be responsive to the need for critical mass in sub-disciplines within biocomputing. For that reason, it is the only meeting whose sessions are defined dynamically each year in response to specific proposals. PSB sessions are organized by leaders of research in biocomputing's “hot topics.” In this way, the meeting provides an early forum for serious examination of emerging methods and approaches in this rapidly changing field.

Detection of Chemical Biological Radiological and Nuclear Agents for the Prevention of Terrorism

Detection of Chemical  Biological  Radiological and Nuclear Agents for the Prevention of Terrorism
Author: Joseph Banoub
Publsiher: Springer
Total Pages: 291
Release: 2014-11-03
ISBN 10: 9401792380
ISBN 13: 9789401792387
Language: EN, FR, DE, ES & NL

Detection of Chemical Biological Radiological and Nuclear Agents for the Prevention of Terrorism Book Review:

This NATO-ASI installment is designed to provide an advanced overview for doctoral and post-doctoral candidates of the state-of-the-art technologies for bio-detection. The main objective of the work aims at providing readers with the latest developments necessary to successfully understand the CBRN Agents and their associated biotechnologies. The core methods focused on are mass spectrometry (including chromatographic and electrophoretic separation) and comparisons to spectroscopic, immunological and molecular analysis of chemical, biological and nuclear agents.

Bioinformatics and Biomedical Engineering

Bioinformatics and Biomedical Engineering
Author: Ignacio Rojas,Olga Valenzuela,Fernando Rojas,Luis Javier Herrera,Francisco Ortuño
Publsiher: Springer Nature
Total Pages: 468
Release: 2022-06-07
ISBN 10: 3031078020
ISBN 13: 9783031078026
Language: EN, FR, DE, ES & NL

Bioinformatics and Biomedical Engineering Book Review:

This volume constitutes the proceedings of the 9th International Work-Conference on IWBBIO 2020, held in Maspalomas, Gran Canaria, Spain, in June 2022. The total of 75 papers presented in the proceedings, was carefully reviewed and selected from 212 submissions. The papers cover the latest ideas and realizations in the foundations, theory, models, and applications for interdisciplinary and multidisciplinary research encompassing disciplines of computer science, mathematics, statistics, biology, bioinformatics, and biomedicine.

Cholesterol Modulation of Protein Function

Cholesterol Modulation of Protein Function
Author: Avia Rosenhouse-Dantsker,Anna N. Bukiya
Publsiher: Springer
Total Pages: 197
Release: 2019-01-16
ISBN 10: 3030042782
ISBN 13: 9783030042783
Language: EN, FR, DE, ES & NL

Cholesterol Modulation of Protein Function Book Review:

In this book, renowned scientists describe the role of steroid chirality and modification of lipid membrane physical properties in the modulation of G protein-coupled receptors and ion channels. The application of commonly-used technical approaches such as mass spectrometry and nucleic magnetic resonance transfer spectroscopy for studies on cholesterol distribution and alteration of lipid bilayer characteristics is also discussed. This book offers comprehensive insights into the current understanding of cholesterol-driven modulation of protein function via mechanisms that extend beyond lipid-protein direct interactions. In the first part, the chapters introduce the reader to the use of the chemical derivatives of cholesterol as a valuable laboratory tool in the studies of cholesterol-driven modulation of protein function. In the second part, examples of cholesterol-induced changes in membrane physical characteristics are presented and discussed in light of their multifaceted contribution to the effect of cholesterol on protein function. The book will be of interest to undergraduate and graduate students as well as basic science and medical researchers with a keen interest in the biophysical properties of cholesterol and physiological consequences of cholesterol presence in biological systems.

Mass Spectrometry Imaging in Food Analysis

Mass Spectrometry Imaging in Food Analysis
Author: Leo M.L. Nollet
Publsiher: CRC Press
Total Pages: 198
Release: 2020-04-29
ISBN 10: 0429765304
ISBN 13: 9780429765308
Language: EN, FR, DE, ES & NL

Mass Spectrometry Imaging in Food Analysis Book Review:

Food contains various compounds and many technologies exist to analyze those molecules of interest. However, the analysis of the spatial distribution of those compounds using conventional technology, such as liquid chromatography-mass spectrometry or gas chromatography-mass spectrometry is difficult. Mass spectrometry imaging (MSI) is a mass spectrometry technique to visualize the spatial distribution of molecules, as biomarkers, metabolites, peptides or proteins by their molecular masses. Despite the fact that MSI has been generally considered a qualitative method, the signal generated by this technique is proportional to the relative abundance of the analyte and so quantification is possible. Mass Spectrometry Imaging in Food Analysis, a volume in the Food Analysis and Properties Series, explains how the novel use of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) will be an ideal complementary approach. MALDI-MSI is a two-dimensional MALDI-MS technology that can detect compounds in a tissue section without extraction, purification, separation, or labeling. It can be used to visualize the spatial distribution of biomolecules in foods. Features: Explains the novel use of matrix-assisted laser desorption/ionization mass spectrometry imaging in food analysis Describes how MALDI-MSI will be a useful technique for optical quality assurance. Shows how MALDI-MSI detects food contaminants and residues Covers the historical development of the technology While there are a multitude of books on mass spectrometry, none focus on food applications and thus this book is ideally suited to food scientists, food industry personnel engaged in product development, research institutions, and universities active in food analysis or chemical analysis. Also available in the Food Analysis and Properties Series: Food Aroma Evolution: During Food Processing, Cooking, and Aging, edited by Matteo Bordiga and Leo M.L. Nollet (ISBN: 9781138338241) Ambient Mass Spectroscopy Techniques in Food and the Environment, edited by Leo M.L. Nollet and Basil K. Munjanja (ISBN: 9781138505568) Hyperspectral Imaging Analysis and Applications for Food Quality, edited by N.C. Basantia, Leo M.L. Nollet, and Mohammed Kamruzzaman (ISBN: 9781138630796) For a complete list of books in this series, please visit our website at: www.crcpress.com/Food-Analysis--Properties/book-series/CRCFOODANPRO

Cancer Metabolomics

Cancer Metabolomics
Author: Shen Hu
Publsiher: Springer Nature
Total Pages: 312
Release: 2021-05-03
ISBN 10: 3030516520
ISBN 13: 9783030516529
Language: EN, FR, DE, ES & NL

Cancer Metabolomics Book Review:

Cancer metabolomics is a rapidly evolving field that aims for a comprehensive dissection of the metabolic phenotypes and functional network of metabolites in human cancers. State of the art metabolomics tools have been developed and applied to studying cancer metabolism and developing metabolic targets for improved diagnosis, prognosis and therapeutic treatment of human cancers. Chapters are written by subject experts in the field of cancer metabolomics with cross-disciplinary contributions. Coverage includes advanced metabolomics technologies and methodologies, including chemical isotope labelling liquid chromatography - mass spectrometry, capillary ion chromatography - mass spectrometry, 2-D gas chromatography – mass spectrometry, capillary electrophoresis – mass spectrometry, nuclear magnetic resonance spectroscopy, shotgun lipidomics, tracer-based metabolomics, microbial metabolomics, mass spectrometry imaging for single cell metabolomics and functional metabolomics. In addition, the book highlights new discoveries in cancer metabolism such as hypoxia inducible factor pathway, isocitrate dehydrogenase 1 mutation and oncometabolites. Finally, contributors focus on the translational applications of metabolomics in human cancers such as glioma, head and neck cancer, and gastric cancer. This new volume will be a unique reference source for cancer researchers and promote applications of metabolomics in understanding cancer metabolism.

Pacific Symposium on Biocomputing

Pacific Symposium on Biocomputing
Author: Anonim
Publsiher: Unknown
Total Pages: 135
Release: 2007
ISBN 10: 1928374650XXX
ISBN 13: UOM:39015069292194
Language: EN, FR, DE, ES & NL

Pacific Symposium on Biocomputing Book Review:

Advances in Imaging and Electron Physics

Advances in Imaging and Electron Physics
Author: Anonim
Publsiher: Academic Press
Total Pages: 440
Release: 2012-12-31
ISBN 10: 0123946352
ISBN 13: 9780123946355
Language: EN, FR, DE, ES & NL

Advances in Imaging and Electron Physics Book Review:

Advances in Imaging and Electron Physics merges two long-running serials--Advances in Electronics and Electron Physics and Advances in Optical and Electron Microscopy. This series features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science and digital image processing, electromagnetic wave propagation, electron microscopy, and the computing methods used in all these domains. Contributions from leading authorities Informs and updates on all the latest developments in the field

Metabolomics Perspectives

Metabolomics Perspectives
Author: Jacopo Troisi
Publsiher: Academic Press
Total Pages: 684
Release: 2022-03-15
ISBN 10: 0323850634
ISBN 13: 9780323850636
Language: EN, FR, DE, ES & NL

Metabolomics Perspectives Book Review:

Metabolomics Perspectives: From Theory to Practical Application is an expertly written volume, which provides a thorough description of the current state-of-the-art in the metabolomics field. The philosophy behind the book is to guide the reader in a step-by-step exploration of metabolomics experiments, ranging from sample preparation to data extraction, analysis and interpretation, and to discuss the main current applications and future perspectives of this emerging science. Armed with critical insights, coupled with a clear writing, the book consists of three main sections. The first one introduces the pivotal theoretical fundamentals and provides a comprehensive overview of the "wet" laboratory workflow, including protocol instructions and a detailed description of experimental methods and analytical techniques. The second section covers a wide range of topics in the context of data analysis, including guidance in exploratory analysis, supervised and unsupervised machine learning approaches and validation and optimization methods. In addition to the several examples reported in the text, the book features an R package, specifically designed to perform all the described algorithms, which is hosted on a companion website (www.metabolomicsperspectives.com) together with several sets of available metabolomic data. Finally, an extensive dissertation describes the latest advances and the major fields of interest for metabolomics applications, highlighting their crucial potentials for future biomedical research. Thus, this book represents a must-read for both experienced researchers, interested in metabolomics, and newcomers to the field. • Provides an in-depth description of the metabolomics experimental workflow and its applications in life science and biomedical research • Features chapter contributions from the greatest international experts in the field • Includes an R package and several sets of metabolomics data, hosted on a companion website

Mass Spectrometry of Nucleosides and Nucleic Acids

Mass Spectrometry of Nucleosides and Nucleic Acids
Author: Joseph H. Banoub,Patrick A. Limbach
Publsiher: CRC Press
Total Pages: 504
Release: 2009-12-21
ISBN 10: 9781420044034
ISBN 13: 1420044036
Language: EN, FR, DE, ES & NL

Mass Spectrometry of Nucleosides and Nucleic Acids Book Review:

Assembling the work of an international panel of researchers, Mass Spectrometry of Nucleosides and Nucleic Acids summarizes and reviews the latest developments in the field and provides a window on the next generation of analysis. Beginning with an overview of recent developments, the book highlights the most popular ionization methods and illustrates the diversity of strategies employed in the characterization and sequencing of DNA and RNA oligomers, nucleosides, nucleotides, and adducts. It describes studies performed on deoxyinosine and its analogues and provides an introduction to tandem mass spectrometry (MS/MS). Next, the contributors examine mass spectrometric application in the study of cyclic nucleotides in biochemical signal transduction. They analyze urinary modified nucleosides and explore DNA adducts. They discuss isotope labeling of DNA-mass spectrometry (ILD-MS) and examine various uses of electrospray ionization mass spectrometry (ESI-MS). The book reviews recent progress in the direct MS characterization of noncovalent nucleic acid-protein complexes, explores the interaction and ionization of guanidine-derived compounds with highly acidic biomolecules, and examines quantitative identification of nucleic acids via signature digestion products detected using mass spectrometry. The book describes a direct-infusion ESI-MS approach that can serve as a screening technique for the presence of modified nucleosides from small RNAs. Lastly, it discusses the LC-MS/MS method for the in vitro replication studies on damage-containing DNA substrates, and concludes with an examination of the influence of metal ions on the structure and reactivity of nucleic acids. The exciting developments in mass spectrometry technology have fueled incredible advances in our understanding of nucleic acids and their complexes. The contributions presented in this volume capture the range of these advances, helping to inspire new findings and avenues of research.

Stimulated Raman Scattering Microscopy

Stimulated Raman Scattering Microscopy
Author: Ji-Xin Cheng,Wei Min,Yasuyuki Ozeki,Dario Polli
Publsiher: Elsevier
Total Pages: 610
Release: 2021-12-04
ISBN 10: 0323903371
ISBN 13: 9780323903370
Language: EN, FR, DE, ES & NL

Stimulated Raman Scattering Microscopy Book Review:

Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics

Bioanalytics

Bioanalytics
Author: Friedrich Lottspeich,Joachim Engels
Publsiher: John Wiley & Sons
Total Pages: 1134
Release: 2018-03-26
ISBN 10: 3527339191
ISBN 13: 9783527339198
Language: EN, FR, DE, ES & NL

Bioanalytics Book Review:

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a birdï¿1⁄2s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Handbook of Rare Earth Elements

Handbook of Rare Earth Elements
Author: Alfred Golloch
Publsiher: Walter de Gruyter GmbH & Co KG
Total Pages: 520
Release: 2022-07-05
ISBN 10: 3110696649
ISBN 13: 9783110696646
Language: EN, FR, DE, ES & NL

Handbook of Rare Earth Elements Book Review:

The reference work describes in its new edition still more up-to-date methods for the recycling and purifi cation processes of rare earth element analysis for industrial and scientific purposes alike. Due to their vast applications, from computer hardware to mobile phones and electric cars, REEs have become a valuable resource for our modern life. New topics: emission spectroscopy, analysis of environmental samples and pharmaceutical applications.

Using Mass Spectrometry for Drug Metabolism Studies Second Edition

Using Mass Spectrometry for Drug Metabolism Studies  Second Edition
Author: Walter A. Korfmacher
Publsiher: CRC Press
Total Pages: 451
Release: 2009-11-03
ISBN 10: 9781420092219
ISBN 13: 1420092219
Language: EN, FR, DE, ES & NL

Using Mass Spectrometry for Drug Metabolism Studies Second Edition Book Review:

The first edition of this now classic work helped to establish mass spectrometry as the premier tool for drug metabolism studies. Completely rewritten from start to finish, Using Mass Spectrometry for Drug Metabolism Studies, Second Edition brings medicinal chemists and mass spectrometry professionals up to speed with the rapid advances in the field, the emergence of cutting-edge approaches, and ways to meet steadily increasing vendor demands. Written by international scientists who are experts in their respective disciplines, this state-of-the-art reference effectively encapsulates current mass spectrometry best practices. The stand-alone chapters cover various topics – from metabolite identification to fast chromatography with UPLC – and in a style that is understandable to experts and field newcomers alike. The second edition of this bestseller includes coverage of new instrumentation and software as well as a wealth of updated information on the latest findings surrounding biomarkers and metabolomics and new chapters on both UPLC and DESI/DART. With more than 180 illustrations and an eight-page color insert, this valuable reference explores multiple modern mass spectrometry techniques and strategies. It includes an excellent overview of the entire drug discovery process plus the latest developments on how mass spectrometry is used to support this endeavor.

Secondary Ion Mass Spectrometry SIMS VII

Secondary Ion Mass Spectrometry  SIMS VII
Author: A. Benninghoven,C. A. Evans,K. D. McKeegan,H. A. Storms,H. W. Werner
Publsiher: Wiley
Total Pages: 992
Release: 1990-06-28
ISBN 10: 1928374650XXX
ISBN 13: UOM:39015019844250
Language: EN, FR, DE, ES & NL

Secondary Ion Mass Spectrometry SIMS VII Book Review:

Based on the proceedings of the Seventh International Conference on Secondary Ion Mass Spectrometry, held in Monterey, California, September 3-8, 1989. Covers fundamental, complementary and enhancement techniques, comparative SIMS, geology, biology, polymers, metallurgy, profiling and semiconductors. Describes a valuable methodology (SIMS) for characterizing solid surfaces and presents numerous analytical applications.

MALDI Mass Spectrometry Imaging

MALDI Mass Spectrometry Imaging
Author: Tiffany Porta Siegel
Publsiher: Royal Society of Chemistry
Total Pages: 500
Release: 2021-11-23
ISBN 10: 1839165200
ISBN 13: 9781839165207
Language: EN, FR, DE, ES & NL

MALDI Mass Spectrometry Imaging Book Review:

This book gathers, in a single resource, knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging. It includes fundamentals in the MALDI ionisation process, different source geometries and capabilities, detection systems and the latest research and applications in the range of –omics area as well as other broader areas. Chapters will touch on dedicated sample preparation protocols specific for the class of compounds of interest, instrumentation used with strengths and current limitations, strategies for structural analysis and identification and applications. It will be a welcomed addition to the literature in this fast-moving field and provide a guide to new innovations and applications especially in metabolomics and proteomics. With contributions from leading experts, this book will be an authoritative guide to this method. Aimed at postgraduate and professional researchers, in academia and in the industrial market where it has direct application to clinical research. It will be a supporting volume for those just entering the field as well as experienced practitioners.

Chemical Imaging Analysis

Chemical Imaging Analysis
Author: Freddy Adams,Carlo Barbante
Publsiher: Elsevier
Total Pages: 480
Release: 2015-06-06
ISBN 10: 0444634509
ISBN 13: 9780444634504
Language: EN, FR, DE, ES & NL

Chemical Imaging Analysis Book Review:

Chemical Imaging Analysis covers the advancements made over the last 50 years in chemical imaging analysis, including different analytical techniques and the ways they were developed and refined to link the composition and structure of manmade and natural materials at the nano/micro scale to the functional behavior at the macroscopic scale. In a development process that started in the early 1960s, a variety of specialized analytical techniques was developed – or adapted from existing techniques – and these techniques have matured into versatile and powerful tools for visualizing structural and compositional heterogeneity. This text explores that journey, providing a general overview of imaging techniques in diverse fields, including mass spectrometry, optical spectrometry including X-rays, electron microscopy, and beam techniques. Provides comprehensive coverage of analytical techniques used in chemical imaging analysis Explores a variety of specialized techniques Provides a general overview of imaging techniques in diverse fields

Applications of Mass Spectrometry Imaging to Cancer

Applications of Mass Spectrometry Imaging to Cancer
Author: Anonim
Publsiher: Academic Press
Total Pages: 312
Release: 2017-01-19
ISBN 10: 0128054409
ISBN 13: 9780128054406
Language: EN, FR, DE, ES & NL

Applications of Mass Spectrometry Imaging to Cancer Book Review:

Applications of Mass Spectrometry Imaging to Cancer, the latest volume in the Advances in Cancer Research provides invaluable information on the exciting and fast-moving field of cancer research. This volume presents original reviews on applications of mass spectrometry imaging to cancer. Provides information on cancer research Offers outstanding and original reviews on a range of cancer research topics Serves as an indispensable reference for researchers and students alike