Identification and Quantification of Drugs Metabolites Drug Metabolizing Enzymes and Transporters

Identification and Quantification of Drugs  Metabolites  Drug Metabolizing Enzymes  and Transporters
Author: Shuguang Ma,Swapan Chowdhury
Publsiher: Elsevier Science
Total Pages: 708
Release: 2020-08-04
ISBN 10: 0128200189
ISBN 13: 9780128200186
Language: EN, FR, DE, ES & NL

Identification and Quantification of Drugs Metabolites Drug Metabolizing Enzymes and Transporters Book Review:

Identification and Quantification of Drugs, Metabolites, Drug Metabolizing Enzymes, and Transporters, Second Edition, is completely updated to provide an overview of the last decade's numerous advances in analytical technologies for detection and quantification of drugs, metabolites, and biomarkers. This new edition goes beyond LC-MS and features all-new chapters on how to evaluate drug absorption, distribution, metabolism, and excretion, potential for hepatic and renal toxicity, immunogenicity of biotherapeutics and translational tools for predicting human dosage, safety and efficacy of small molecules and biologics. This book will be an important handbook and desk reference for pharmacologists, toxicologists, clinical scientists, and students interested in the fields of pharmacology, biochemistry, and drug metabolism. Four sections in the book with 24 chapters give readers an overview of state-of-the-art techniques for identifying and quantifying drugs, metabolites and biomarkers, including a chapter on new approaches for quantification of enzymes and transporters in different tissues Focuses on the role of drug metabolism enzymes, transporters in disposition and drug-drug interactions, as well as strategies for evaluating drug metabolism and safety using advanced liver and kidney models. Discussions on immunogenicity risks of biologics and their evaluation methods have been included Includes several chapters on advanced translational sciences to predict human dosage, pharmacokinetics and efficacy for small molecules and biotherapeutics All chapters are written by experts with a wide range of practical experience from the industry and academia

Identification and Quantification of Drugs Metabolites and Metabolizing Enzymes by LC MS

Identification and Quantification of Drugs  Metabolites and Metabolizing Enzymes by LC MS
Author: Swapan Chowdhury
Publsiher: Elsevier
Total Pages: 354
Release: 2005-11-04
ISBN 10: 9780080456065
ISBN 13: 0080456065
Language: EN, FR, DE, ES & NL

Identification and Quantification of Drugs Metabolites and Metabolizing Enzymes by LC MS Book Review:

As new techniques of transferring from liquid to gas phase and measuring masses of drug molecules and metabolites become more prevalent, so do the technical challenges of putting these techniques into proper use, as well as the task of consolidating emerging applications. Identification and Quantification of Drugs, Metabolites and Metabolizing Enzymes by LC-MS, Volume 6 fills the gap in the lack of presently available literature by providing a critical review in the current use of liquid chromatography-mass spectrometry (LC-MS) in drug discovery and development. With chapters written by experts with a wide range of practical experience from the pharmaceutical industry, emphasis is placed on techniques and applications. The book also includes chapters on how to utilize LC-MS instrumentation for current drug metabolism problems. This book is intended for those beginning to use LC-MS for drug metabolism studies as well as for those considered advanced practitioners. * Introduces readers to the practical applications of modern liquid chromatography-mass spectrometry (LC-MS) in a wide range of drug metabolism studies· * Provides a comprehensive description of different forms of metabolites, with detailed discussion on the wide range of methodologies used to identify them * Highlights problems associated with drug quantification and offers practical solutions

Identification and Quantification of Drugs Metabolites Drug Metabolizing Enzymes and Transporters

Identification and Quantification of Drugs  Metabolites  Drug Metabolizing Enzymes  and Transporters
Author: Shuguang Ma,Swapan Chowdhury
Publsiher: Elsevier
Total Pages: 708
Release: 2020-07-09
ISBN 10: 0128200197
ISBN 13: 9780128200193
Language: EN, FR, DE, ES & NL

Identification and Quantification of Drugs Metabolites Drug Metabolizing Enzymes and Transporters Book Review:

Identification and Quantification of Drugs, Metabolites, Drug Metabolizing Enzymes, and Transporters, Second Edition, is completely updated to provide an overview of the last decade’s numerous advances in analytical technologies for detection and quantification of drugs, metabolites, and biomarkers. This new edition goes beyond LC-MS and features all-new chapters on how to evaluate drug absorption, distribution, metabolism, and excretion, potential for hepatic and renal toxicity, immunogenicity of biotherapeutics and translational tools for predicting human dosage, safety and efficacy of small molecules and biologics. This book will be an important handbook and desk reference for pharmacologists, toxicologists, clinical scientists, and students interested in the fields of pharmacology, biochemistry, and drug metabolism. Four sections in the book with 24 chapters give readers an overview of state-of-the-art techniques for identifying and quantifying drugs, metabolites and biomarkers, including a chapter on new approaches for quantification of enzymes and transporters in different tissues Focuses on the role of drug metabolism enzymes, transporters in disposition and drug-drug interactions, as well as strategies for evaluating drug metabolism and safety using advanced liver and kidney models. Discussions on immunogenicity risks of biologics and their evaluation methods have been included Includes several chapters on advanced translational sciences to predict human dosage, pharmacokinetics and efficacy for small molecules and biotherapeutics All chapters are written by experts with a wide range of practical experience from the industry and academia

Transporters and Drug Metabolizing Enzymes in Drug Toxicity

Transporters and Drug Metabolizing Enzymes in Drug Toxicity
Author: Albert P. Li
Publsiher: John Wiley & Sons
Total Pages: 528
Release: 2021-06-29
ISBN 10: 1119170990
ISBN 13: 9781119170990
Language: EN, FR, DE, ES & NL

Transporters and Drug Metabolizing Enzymes in Drug Toxicity Book Review:

This book provides a comprehensive and up-to-date coverage of the relationship between drug metabolism enzymes and transporters on drug toxicity, along with methods to investigate their role on adverse drug reactions. Unites both the metabolism and transporter components of drug toxicity – two aspects not normally connected and the latter often neglected Familiarizes readers with the mechanism and species differences in drug metabolizing enzymes and transporters Discusses promising approaches to accurately predict human drug toxicity via the incorporation of human drug metabolism in toxicity evaluation

Drug Transporters

Drug Transporters
Author: Martin F. Fromm,Richard B. Kim
Publsiher: Springer Science & Business Media
Total Pages: 454
Release: 2010-11-19
ISBN 10: 3642145418
ISBN 13: 9783642145414
Language: EN, FR, DE, ES & NL

Drug Transporters Book Review:

It is increasingly recognized that various transporter proteins are expressed throughout the body and determine absorption, tissue distribution, biliary and renal elimination of endogenous compounds and drugs and drug effects. This book will give an overview on the transporter families which are most important for drug therapy. Most chapters will focus on one transporter family highlighting tissue expression, substrates, inhibitors, knock-out mouse models and clinical studies.

Biodegradation and Biodeterioration at the Nanoscale

Biodegradation and Biodeterioration at the Nanoscale
Author: Hafiz M.N. Iqbal,Muhammad Bilal,Tuan Anh Nguyen,Ghulam Yasin
Publsiher: Elsevier
Total Pages: 750
Release: 2021-10-08
ISBN 10: 0128241799
ISBN 13: 9780128241790
Language: EN, FR, DE, ES & NL

Biodegradation and Biodeterioration at the Nanoscale Book Review:

Biodegradation and Biodeterioration at the Nanoscale describes the biodegradation and biodeterioration of materials in the presence of nanomaterials. The book's chapters focus on the basic principles, action mechanisms and promising applications of advanced nanomaterials, along with their integration with biotechnological processes for controlled degradation and deterioration of materials. In addition, the current research indications, positive or negative environmental impacts, legislation and future directions are also discussed. This book is an important reference source for researchers, engineers and scientists working in environmental remediation, biotechnology, materials science, corrosion and nanotechnology. Provides detailed coverage on how nano-biomaterials degrade and deteriorate Compares how different types of bionanomaterials decompose Explains how the priorities of bionanomaterials affect their deterioration rate

Recent Advances in Analytical Techniques Volume 5

Recent Advances in Analytical Techniques  Volume 5
Author: Atta-ur-Rahman,Sibel Ozkan
Publsiher: Bentham Science Publishers
Total Pages: 300
Release: 2022-01-05
ISBN 10: 9815036947
ISBN 13: 9789815036947
Language: EN, FR, DE, ES & NL

Recent Advances in Analytical Techniques Volume 5 Book Review:

Recent Advances in Analytical Techniques is a series of updates in techniques used in chemical analysis. Each volume presents a selection of chapters that explain different analytical techniques and their use in applied research. Readers will find updated information about developments in analytical methods such as chromatography, electrochemistry, optical sensor arrays for pharmaceutical and biomedical analysis. The fifth volume of the series features five reviews which demonstrate chemical analysis techniques applied in different disciplines. - Superior Aspects of Liquid Chromatography-Based Mass Spectrometers in Chiral Analysis - New Trends in Sample Preparation for Pharmaceutical and Biological Analysis by Chromatographic Methods - Qualitative and Quantitative Investigation of Bio Tissues using Microscopy and Data Mining - Analytical Techniques For Analysis of Metals and Minerals in The Soil Samples - Monitoring Therapeutic Response in Cancers: A Raman Spectroscopy Approach

Cerebral endothelial and glial cells are more than bricks in the Great Wall of the brain insights into the way the blood brain barrier actually works Celebrating the centenary of Goldman s experiments

Cerebral endothelial and glial cells are more than bricks in the Great Wall of the brain  insights into the way the blood brain barrier actually works  Celebrating the centenary of Goldman   s experiments
Author: Elena García-Martín,George E. Barreto,José A. G. Agúndez,Rubem C. A. Guedes,Ramon Santos El-Bachá
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2015-07-02
ISBN 10: 2889195724
ISBN 13: 9782889195725
Language: EN, FR, DE, ES & NL

Cerebral endothelial and glial cells are more than bricks in the Great Wall of the brain insights into the way the blood brain barrier actually works Celebrating the centenary of Goldman s experiments Book Review:

When Ehrlich discovered the first evidence of the blood-brain barrier in 1885, he probably did not perceive the Great Wall that remained hidden from consciousness inside the central nervous system. Ehrlich had observed that acidic vital dyes did not stain the brain if they were injected into the blood stream. A century ago (1913), Goldman showed that the injection of trypan blue in the cerebrospinal fluid stained only the brain, but not the other organs. For almost a century it was thought that the blood-brain barrier (BBB) consisted in a physical barrier, resulting from the restricted permeability of the cerebral endothelial cell layer, as they are joined by tight junctions. However, as scientists are always looking for news in what is already discovered, in the end of the 20th century we had evidences that cerebral endothelial and glial cells express several drug metabolizing enzymes consisting in a second protection system: a metabolic barrier. Furthermore, the drugs and their metabolites must overcome the activity of several multidrug resistance proteins that function as ATP-dependent efflux pumps, consisting in the third line of defence: the active barrier. Therefore, the way the BBB actually works should be better explained. Several endogenous compounds, as well as xenobiotics, may be activated by enzymes of the metabolic barrier, generating reactive oxygen species that could damage neurons. Therefore, endothelial and glial cells possess endogenous protecting compounds and enzymes against oxidants, consisting in an antioxidant barrier. When all these systems fail, glial cells, mainly microglia, secrete cytokines in an attempt to crosstalk with defence cells asking for help, which consists in an immune barrier. In cerebral regions that are devoid of the physical barrier, such as circumventricular organs, the metabolic, active, antioxidant and immune barriers are reinforced. It is important to understand how cells involved in the BBB interact with one another and the dynamic mechanisms of their functions. This Research Topic published in this e-Book considers recent highlights in BBB structure, cell and molecular biology, biotransformation, physiology, pathology, pharmacology, immunology and how these basic knowledges can be applied in drug discovery and clinical researches, rewriting what is already written, and paving the way that goes to the Great Wall in the Frontiers of the Brain in this new century that is just beginning.

Drug Metabolism in Drug Design and Development

Drug Metabolism in Drug Design and Development
Author: Donglu Zhang,Mingshe Zhu,William G. Humphreys
Publsiher: John Wiley & Sons
Total Pages: 448
Release: 2007-11-16
ISBN 10: 9780470191682
ISBN 13: 0470191686
Language: EN, FR, DE, ES & NL

Drug Metabolism in Drug Design and Development Book Review:

The essentials of drug metabolism vital to developing new therapeutic entities Information on the metabolism and disposition of candidate drugs is a critical part of all aspects of the drug discovery and development process. Drug metabolism, as practiced in the pharmaceutical industry today, is a complex, multidisciplinary field that requires knowledge of sophisticated analytical technologies and expertise in mechanistic and kinetic enzymology, organic reaction mechanism, pharmacokinetic analysis, animal physiology, basic chemical toxicology, preclinical pharmacology, and molecular biology. With chapters contributed by experts in their specific areas, this reference covers: * Basic concepts of drug metabolism * The role of drug metabolism in the pharmaceutical industry * Analytical techniques in drug metabolism * Common experimental approaches and protocols Drug Metabolism in Drug Design and Development emphasizes practical considerations such as the data needed, the experiments and analytical methods typically employed, and the interpretation and application of data. Chapters highlight facts, common protocols, detailed experimental designs, applications, and limitations of techniques. This is a comprehensive, hands-on reference for drug metabolism researchers as well as other professionals involved in pre-clinical drug discovery and development.

Early Drug Development

Early Drug Development
Author: Mitchell N. Cayen
Publsiher: John Wiley & Sons
Total Pages: 654
Release: 2011-02-25
ISBN 10: 9781118035207
ISBN 13: 1118035208
Language: EN, FR, DE, ES & NL

Early Drug Development Book Review:

The focus of early drug development has been the submission of an Investigational New Drug application to regulatory agencies. Early Drug Development: Strategies and Routes to First-in-Human Trials guides drug development organizations in preparing and submitting an Investigational New Drug (IND) application. By explaining the nuts and bolts of preclinical development activities and their interplay in effectively identifying successful clinical candidates, the book helps pharmaceutical scientists determine what types of discovery and preclinical research studies are needed in order to support a submission to regulatory agencies.

Drug Metabolism Pharmacokinetics and Bioanalysis

Drug Metabolism  Pharmacokinetics and Bioanalysis
Author: Hye Suk Lee,Kwang-Hyeon Liu
Publsiher: MDPI
Total Pages: 230
Release: 2019-06-12
ISBN 10: 3038979163
ISBN 13: 9783038979166
Language: EN, FR, DE, ES & NL

Drug Metabolism Pharmacokinetics and Bioanalysis Book Review:

Drug metabolism/pharmacokinetics and drug interaction studies have been extensively carried out in order to secure the druggability and safety of new chemical entities throughout the development of new drugs. Recently, drug metabolism and transport by phase II drug metabolizing enzymes and drug transporters, respectively, as well as phase I drug metabolizing enzymes, have been studied. A combination of biochemical advances in the function and regulation of drug metabolizing enzymes and automated analytical technologies are revolutionizing drug metabolism research. There are also potential drug–drug interactions with co-administered drugs due to inhibition and/or induction of drug metabolic enzymes and drug transporters. In addition, drug interaction studies have been actively performed to develop substrate cocktails that do not interfere with each other and a simultaneous analytical method of substrate drugs and their metabolites using a tandem mass spectrometer. This Special Issue has the aim of highlighting current progress in drug metabolism/pharmacokinetics, drug interactions, and bioanalysis.

Reactive Drug Metabolites

Reactive Drug Metabolites
Author: Amit S. Kalgutkar,Deepak Dalvie,R. Scott Obach,Dennis A. Smith
Publsiher: John Wiley & Sons
Total Pages: 402
Release: 2012-09-06
ISBN 10: 352765576X
ISBN 13: 9783527655762
Language: EN, FR, DE, ES & NL

Reactive Drug Metabolites Book Review:

Closing a gap in the scientifi c literature, this first comprehensive introduction to the topic is based on current best practice in one of the largest pharmaceutical companies worldwide. The first chapters trace the development of our understanding of drug metabolite toxicity, covering basic concepts and techniques in the process, while the second part details chemical toxicophores that are prone to reactive metabolite formation. This section also reviews the various drug-metabolizing enzymes that can participate in catalyzing reactive metabolite formation, including a discussion of the structure-toxicity relationships for drugs. Two chapters are dedicated to the currently hot topics of herbal constituents and IADRs. The next part covers current strategies and approaches to evaluate the reactive metabolite potential of new drug candidates, both by predictive and by bioanalytical methods. There then follows an in-depth analysis of the toxicological potential of the top 200 prescription drugs, illustrating the power and the limits of the toxicophore concept, backed by numerous case studies. Finally, a risk-benefi t approach to managing the toxicity risk of reactive metabolite-prone drugs is presented. Since the authors carefully develop the knowledge needed, from fundamental considerations to current industry standards, no degree in pharmacology is required to read this book, making it perfect for medicinal chemists without in-depth pharmacology training.

Drug Drug Interactions

Drug Drug Interactions
Author: A. David Rodrigues
Publsiher: CRC Press
Total Pages: 768
Release: 2019-01-03
ISBN 10: 0849375940
ISBN 13: 9780849375941
Language: EN, FR, DE, ES & NL

Drug Drug Interactions Book Review:

Authored by renowned leaders in the field, this comprehensive volume covers all aspects of drug-drug interactions, including preclinical, clinical, toxicological, and regulatory perspectives.Thoroughly updated, this second edition reflects the significant advances and includes extensive new material on:key interplay between transporters and enzymes

Handbook of Radioactivity Analysis

Handbook of Radioactivity Analysis
Author: Michael F. L'Annunziata
Publsiher: Academic Press
Total Pages: 1074
Release: 2020-03-07
ISBN 10: 0128143967
ISBN 13: 9780128143964
Language: EN, FR, DE, ES & NL

Handbook of Radioactivity Analysis Book Review:

Handbook of Radioactivity Analysis: Radiation Physics and Detectors, Volume One, and Radioanalytical Applications, Volume Two, Fourth Edition, constitute an authoritative reference on the principles, practical techniques and procedures for the accurate measurement of radioactivity - everything from the very low levels encountered in the environment, to higher levels measured in radioisotope research, clinical laboratories, biological sciences, radionuclide standardization, nuclear medicine, nuclear power, and fuel cycle facilities, and in the implementation of nuclear forensic analysis and nuclear safeguards. It includes sample preparation techniques for all types of matrices found in the environment, including soil, water, air, plant matter and animal tissue, and surface swipes. Users will find the latest advances in the applications of radioactivity analysis across various fields, including environmental monitoring, radiochemical standardization, high-resolution beta imaging, automated radiochemical separation, nuclear forensics, and more. Spans two volumes, Radiation Physics and Detectors and Radioanalytical Applications Includes a new chapter on the analysis of environmental radionuclides Provides the latest advances in the applications of liquid and solid scintillation analysis, alpha- and gamma spectrometry, mass spectrometric analysis, Cherenkov counting, flow-cell radionuclide analysis, radionuclide standardization, aerosol analysis, high-resolution beta imaging techniques, analytical techniques in nuclear forensics, and nuclear safeguards Describes the timesaving techniques of computer-controlled automatic separation and activity analysis of radionuclides Provides an extensive table of the radiation characteristics of most radionuclides of interest for the radioanalytical chemist

Topics on Drug Metabolism

Topics on Drug Metabolism
Author: James Paxton
Publsiher: BoD – Books on Demand
Total Pages: 308
Release: 2012-02-22
ISBN 10: 9535100998
ISBN 13: 9789535100997
Language: EN, FR, DE, ES & NL

Topics on Drug Metabolism Book Review:

In order to avoid late-stage drug failure due to factors such as undesirable metabolic instability, toxic metabolites, drug-drug interactions, and polymorphic metabolism, an enormous amount of effort has been expended by both the pharmaceutical industry and academia towards developing more powerful techniques and screening assays to identify the metabolic profiles and enzymes involved in drug metabolism. This book presents some in-depth reviews of selected topics in drug metabolism. Among the key topics covered are: the interplay between drug transport and metabolism in oral bioavailability; the influence of genetic and epigenetic factors on drug metabolism; impact of disease on transport and metabolism; and the use of novel microdosing techniques and novel LC/MS and genomic technologies to predict the metabolic parameters and profiles of potential new drug candidates.

Drug Discovery and Evaluation Safety and Pharmacokinetic Assays

Drug Discovery and Evaluation  Safety and Pharmacokinetic Assays
Author: H. Gerhard Vogel,Jochen Maas,Franz J. Hock,Dieter Mayer
Publsiher: Springer
Total Pages: 1404
Release: 2013-02-27
ISBN 10: 9783642252396
ISBN 13: 3642252397
Language: EN, FR, DE, ES & NL

Drug Discovery and Evaluation Safety and Pharmacokinetic Assays Book Review:

-A landmark in the continuously changing world of drugs -Essential reading for scientists and managers in the pharmaceutical industry involved in drug finding, drug development and decision making in the development process -Of use for government institutions and committees working on official guidelines for drug evaluation worldwide

Handbook of Drug Nutrient Interactions

Handbook of Drug Nutrient Interactions
Author: Joseph I. Boullata,Vincent T. Armenti
Publsiher: Springer Science & Business Media
Total Pages: 824
Release: 2010-03-17
ISBN 10: 160327362X
ISBN 13: 9781603273626
Language: EN, FR, DE, ES & NL

Handbook of Drug Nutrient Interactions Book Review:

Handbook of Drug-Nutrient Interactions, Second Edition is an essential new work that provides a scientific look behind many drug-nutrient interactions, examines their relevance, offers recommendations, and suggests research questions to be explored. In the five years since publication of the first edition of the Handbook of Drug-Nutrient Interactions new perspectives have emerged and new data have been generated on the subject matter. Providing both the scientific basis and clinical relevance with appropriate recommendations for many interactions, the topic of drug-nutrient interactions is significant for clinicians and researchers alike. For clinicians in particular, the book offers a guide for understanding, identifying or predicting, and ultimately preventing or managing drug-nutrient interactions to optimize patient care. Divided into six sections all chapters have been revised or are new to this edition. Chapters balance the most technical information with practical discussions and include outlines that reflect the content; discussion questions that can guide the reader to the critical areas covered in each chapter, complete definitions of terms with the abbreviation fully defined and consistent use of terms between chapters. The editors have performed an outstanding service to clinical pharmacology and pharmaco-nutrition by bringing together a multi-disciplinary group of authors. Handbook of Drug-Nutrient Interactions, Second Edition is a comprehensive up-to-date text for the total management of patients on drug and/or nutrition therapy but also an insight into the recent developments in drug-nutrition interactions which will act as a reliable reference for clinicians and students for many years to come.

Psychopharmacology Abstracts

Psychopharmacology Abstracts
Author: Anonim
Publsiher: Unknown
Total Pages: 135
Release: 1976
ISBN 10: 1928374650XXX
ISBN 13: IND:30000090299557
Language: EN, FR, DE, ES & NL

Psychopharmacology Abstracts Book Review:

Drug Transporters in Drug Disposition Effects and Toxicity

Drug Transporters in Drug Disposition  Effects and Toxicity
Author: Xiaodong Liu,Guoyu Pan
Publsiher: Springer Nature
Total Pages: 580
Release: 2019-09-30
ISBN 10: 9811376476
ISBN 13: 9789811376474
Language: EN, FR, DE, ES & NL

Drug Transporters in Drug Disposition Effects and Toxicity Book Review:

This book provides with a comprehensive overview of the role of drug transporters in drug disposition and efficacy/toxicity, as well as drug-drug interactions and recent advances in the field. Transporters are known determinants of drug disposition and efficacy/toxicity. In general, they are divided into solute carrier (SLC) and ATP binding cassette (ABC) families, and are located along cell membranes, where they mediate drug uptake into cells and export out of cells. Drug transporters are essential in maintaining cell homeostasis, and their gene mutations may cause or contribute to severe human genetic disorders, such as cystic fibrosis, neurological disease, retinal degeneration, anemia, and cholesterol and bile transport defects. Conversely, some diseases may also alter transporter functions and expressions, in turn aggravating disease process. Further, since over-expression of some ABC transporters is a potential contributor to multidrug-resistance (MDR), the book presents a number of strategies to overcome MDR, including ABC transporter inhibitors and applying epigenetic methods to modulate transporter expressions and functions. This book is useful for graduate students and professionals who are looking to refresh or expand their knowledge of this exciting field.

LC MS MS in Proteomics

LC MS MS in Proteomics
Author: Pedro R. Cutillas,John F. Timms
Publsiher: Humana Press
Total Pages: 330
Release: 2011-04-06
ISBN 10: 9781607617815
ISBN 13: 1607617811
Language: EN, FR, DE, ES & NL

LC MS MS in Proteomics Book Review:

With the development of new quantitative strategies and powerful bioinformatics tools to cope with the analysis of the large amounts of data generated in proteomics experiments, liquid chromatography with tandem mass spectrometry (LC-MS/MS) is making possible the analysis of proteins on a global scale, meaning that proteomics can now start competing with cDNA microarrays for the analysis of whole genomes. In LC-MS/MS in Proteomics: Methods and Applications, experts in the field provide protocols and up-to-date reviews of the applications of LC-MS/MS, with a particular focus on MS-based methods of protein and peptide quantification and the analysis of post-translational modifications. Beginning with overviews of the use of LC-M/MS in protein analysis, the book continues with topics such as protocols for the analysis of post-translational modifications, with particular focus on phosphorylation and glycosylation, popular techniques for quantitative proteomics, such as multiple reaction monitoring, metabolic labelling, and chemical tagging, biomarker discovery in biological fluids, as well as novel applications of LC-MS/MS. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, LC-MS/MS in Proteomics: Methods and Applications presents the techniques and concepts necessary in order to aid proteomic practitioners in the application of LC-MS/MS to essentially any biological problem.