Molecular Biology Techniques

Molecular Biology Techniques
Author: Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
Publsiher: Academic Press
Total Pages: 200
Release: 2012
ISBN 10: 0123855446
ISBN 13: 9780123855442
Language: EN, FR, DE, ES & NL

Molecular Biology Techniques Book Review:

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology
Author: Stefan Surzycki
Publsiher: Springer
Total Pages: 434
Release: 2000-04-27
ISBN 10: 9783540666783
ISBN 13: 3540666788
Language: EN, FR, DE, ES & NL

Basic Techniques in Molecular Biology Book Review:

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Techniques in Molecular Biology

Techniques in Molecular Biology
Author: John M. Walker,Wim Gaastra
Publsiher: Springer
Total Pages: 332
Release: 1987-01-22
ISBN 10: 9780709936732
ISBN 13: 0709936737
Language: EN, FR, DE, ES & NL

Techniques in Molecular Biology Book Review:

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly intro duced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have con tributed a wide range of new techniques. In Volume 1, published in 1983, we introduced the reader to a selection of the more advanced analytical and preparative techniques which we considered to be frequently used by research workers in the field of molecular biology. In choosing techniques for Volume 1 we obviously had to be selective and were unable to cover as broad a spectrum of techniques as we would have liked. However, the pro duction of Volume 2 has allowed us to develop the theme initiated in Volume 1 and also expand to include a wider range of subject areas. As with Volume 1, the majority of chapters relate to nucleic acid method ology, but we have also covered immunological methodology and protein 1. Obviously, we purification techniques that were not included in Volume see Volume 2 as simply a continuation of Volume 1. As with Volume 1, a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new termin ology, a glossary of terms has been included.

Techniques for Molecular Biology

Techniques for Molecular Biology
Author: D Tagu
Publsiher: CRC Press
Total Pages: 230
Release: 2006-01-04
ISBN 10: 148229446X
ISBN 13: 9781482294460
Language: EN, FR, DE, ES & NL

Techniques for Molecular Biology Book Review:

This book explains the theoretical principles of numerous techniques of genomic studies developed recently in laboratories. The book is designed for any reader who is curious to know the bases of different techniques of manipulation of nucleic acids.

Basic Methods in Molecular Biology

Basic Methods in Molecular Biology
Author: Leonard Davis
Publsiher: Elsevier
Total Pages: 399
Release: 2012-12-02
ISBN 10: 044460149X
ISBN 13: 9780444601490
Language: EN, FR, DE, ES & NL

Basic Methods in Molecular Biology Book Review:

Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
Author: Keith Wilson,John Walker
Publsiher: Cambridge University Press
Total Pages: 135
Release: 2010-03-04
ISBN 10: 1107393493
ISBN 13: 9781107393493
Language: EN, FR, DE, ES & NL

Principles and Techniques of Biochemistry and Molecular Biology Book Review:

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Practical Techniques in Molecular Biotechnology

Practical Techniques in Molecular Biotechnology
Author: Bal Ram Singh,Raj Kumar
Publsiher: Cambridge University Press
Total Pages: 500
Release: 2021-10-31
ISBN 10: 1108486401
ISBN 13: 9781108486408
Language: EN, FR, DE, ES & NL

Practical Techniques in Molecular Biotechnology Book Review:

The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.

Techniques in Free Radical Research

Techniques in Free Radical Research
Author: A.T. Diplock,M.C.R. Symons,C.A. Rice-Evans
Publsiher: Elsevier
Total Pages: 290
Release: 1991-12-11
ISBN 10: 9780080858913
ISBN 13: 0080858910
Language: EN, FR, DE, ES & NL

Techniques in Free Radical Research Book Review:

Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage. Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.

Advances in Molecular Techniques

Advances in Molecular Techniques
Author: Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
Publsiher: CRC Press
Total Pages: 436
Release: 2018-05-11
ISBN 10: 1351021206
ISBN 13: 9781351021203
Language: EN, FR, DE, ES & NL

Advances in Molecular Techniques Book Review:

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Microfluidic Methods for Molecular Biology

Microfluidic Methods for Molecular Biology
Author: Chang Lu,Scott S. Verbridge
Publsiher: Springer
Total Pages: 376
Release: 2016-05-14
ISBN 10: 3319300199
ISBN 13: 9783319300191
Language: EN, FR, DE, ES & NL

Microfluidic Methods for Molecular Biology Book Review:

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Biological Aging

Biological Aging
Author: Trygve O. Tollefsbol
Publsiher: Springer Science & Business Media
Total Pages: 414
Release: 2007
ISBN 10: 1597453617
ISBN 13: 9781597453615
Language: EN, FR, DE, ES & NL

Biological Aging Book Review:

The goal of Biological Aging: Methods and Protocols is to present some of the most promising and important tools that are currently used in biological aging research. These tools include established protocols such as aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies and the use of ribozymes in aging research. Collectively, these powerful tools combined with the many other techniques that are presented are rapidly advancing the exciting and expanding field of biological aging.

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology
Author: Rajan Katoch
Publsiher: Springer Science & Business Media
Total Pages: 441
Release: 2011-07-19
ISBN 10: 9781441997852
ISBN 13: 1441997857
Language: EN, FR, DE, ES & NL

Analytical Techniques in Biochemistry and Molecular Biology Book Review:

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Protocols used in Molecular Biology

Protocols used in Molecular Biology
Author: Sandeep Singh,Dhiraj Kumar
Publsiher: Bentham Science Publishers
Total Pages: 185
Release: 2020-01-23
ISBN 10: 981143929X
ISBN 13: 9789811439292
Language: EN, FR, DE, ES & NL

Protocols used in Molecular Biology Book Review:

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
Author: Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publsiher: Academic Press
Total Pages: 218
Release: 2020-11-10
ISBN 10: 012824450X
ISBN 13: 9780128244500
Language: EN, FR, DE, ES & NL

Advanced Methods in Molecular Biology and Biotechnology Book Review:

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology
Author: Andreas Hofmann,Samuel Clokie
Publsiher: Cambridge University Press
Total Pages: 962
Release: 2018-03-31
ISBN 10: 1107162270
ISBN 13: 9781107162273
Language: EN, FR, DE, ES & NL

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology Book Review:

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Fundamentals and Techniques of Biophysics and Molecular Biology

Fundamentals and Techniques of Biophysics and Molecular Biology
Author: Pranav Kumar
Publsiher: Pathfinder Publication unit of PAPL
Total Pages: 236
Release: 2021
ISBN 10: 938047315X
ISBN 13: 9789380473154
Language: EN, FR, DE, ES & NL

Fundamentals and Techniques of Biophysics and Molecular Biology Book Review:

Fundamentals and Techniques of Biophysics and Molecular Biology textbook has the primary goal to teach students about theoretical principles and applications of the key biophysical and molecular methods used in biochemistry and molecular biology. A substantial theoretical basis has been covered to understand key experimental techniques such as Chromatography, Electrophoresis, Spectroscopy, Mass spectrometry, Centrifugation, Microscopy, Flow cytometry, Chromatin immunoprecipitation, Immunotechniques, FRET and FRAP, Polymerase chain reaction, Phage display, Yeast two-hybrid assay, DNA sequencing, Biosensors, CRISPR/Cas systems so that students can make appropriate choices and efficient use of techniques. The most significant feature of this book is its clear, up-to-date and accurate explanations of mechanisms, rather than the mere description of facts and events. This book is published by Pathfinder Publication, New Delhi, India.

Molecular Biology Techniques

Molecular Biology Techniques
Author: Walt Ream,Katharine G. Field
Publsiher: Academic Press
Total Pages: 234
Release: 1998-11-17
ISBN 10: 0080536824
ISBN 13: 9780080536828
Language: EN, FR, DE, ES & NL

Molecular Biology Techniques Book Review:

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Phosphoinositides

Phosphoinositides
Author: Roberto J. Botelho
Publsiher: Humana
Total Pages: 239
Release: 2021-01-23
ISBN 10: 9781071611418
ISBN 13: 1071611410
Language: EN, FR, DE, ES & NL

Phosphoinositides Book Review:

This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.

Computational Genome Analysis

Computational Genome Analysis
Author: Richard C. Deonier,Simon Tavaré,Michael S. Waterman
Publsiher: Springer Science & Business Media
Total Pages: 535
Release: 2005-12-27
ISBN 10: 0387288074
ISBN 13: 9780387288079
Language: EN, FR, DE, ES & NL

Computational Genome Analysis Book Review:

This book presents the foundations of key problems in computational molecular biology and bioinformatics. It focuses on computational and statistical principles applied to genomes, and introduces the mathematics and statistics that are crucial for understanding these applications. The book features a free download of the R software statistics package and the text provides great crossover material that is interesting and accessible to students in biology, mathematics, statistics and computer science. More than 100 illustrations and diagrams reinforce concepts and present key results from the primary literature. Exercises are given at the end of chapters.

Genetics and Molecular Biology

Genetics and Molecular Biology
Author: Robert F. Schleif
Publsiher: Unknown
Total Pages: 698
Release: 1993
ISBN 10: 1928374650XXX
ISBN 13: UOM:39015039112068
Language: EN, FR, DE, ES & NL

Genetics and Molecular Biology Book Review:

In the first edition of Genetics and Molecular Biology, renowned researcher and award-winning teacher Robert Schleif produced a unique and stimulating text that was a notable departure from the standard compendia of facts and observations. Schleif's strategy was to present the underlying fundamental concepts of molecular biology with clear explanations and critical analysis of well-chosen experiments. The result was a concise and practical approach that offered students a real understanding of the subject. This second edition retains that valuable approach--with material thoroughly updated to include an integrated treatment of prokaryotic and eukaryotic molecular biology. Genetics and Molecular Biology is copiously illustrated with two-color line art. Each chapter includes an extensive list of important references to the primary literature, as well as many innovative and thought-provoking problems on material covered in the text or on related topics. These help focus the student's attention of a variety of critical issues. Solutions are provided for half of the problems. Praise for the first edition: "Schleif's Genetics and Molecular Biology... is a remarkable achievement. It is an advanced text, derived from material taught largely to postgraduates, and will probably be thought best suited to budding professionals in molecular genetics. In some ways this would be a pity, because there is also gold here for the rest of us... The lessons here in dealing with the information explosion in biology are that an ounce of rationale is worth a pound of facts and that, for educational value, there is nothing to beat an author writing about stuff he knows from theinside."--Nature. "Schleif presents a quantitative, chemically rigorous approach to analyzing problems in molecular biology. The text is unique and clearly superior to any currently available."--R.L. Bernstein, San Francisco State University. "The greatest strength is the author's ability to challenge the student to become involved and get below the surface."--Clifford Brunk, UCLA