Mass Spectrometry in Biophysics

Mass Spectrometry in Biophysics
Author: Igor A. Kaltashov,Stephen J. Eyles
Publsiher: John Wiley & Sons
Total Pages: 320
Release: 2005-05-06
ISBN 10: 0471705160
ISBN 13: 9780471705161
Language: EN, FR, DE, ES & NL

Mass Spectrometry in Biophysics Book Review:

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

Mass Spectrometry in Structural Biology and Biophysics

Mass Spectrometry in Structural Biology and Biophysics
Author: Igor A. Kaltashov,Stephen J. Eyles
Publsiher: John Wiley & Sons
Total Pages: 289
Release: 2012-04-03
ISBN 10: 0470937793
ISBN 13: 9780470937792
Language: EN, FR, DE, ES & NL

Mass Spectrometry in Structural Biology and Biophysics Book Review:

With its detailed and systematic coverage of the current state of biophysical mass spectrometry (MS), here is one of the first systematic presentations of the full experimental array of MS-based techniques used in biophysics, covering both fundamental and practical issues. The book presents a discussion of general biophysical concepts and a brief overview of traditional biophysical techniques before outlining the more advanced concepts of mass spectrometry. The new edition gives an up-to-date and expanded coverage of experimental methodologies and a clear look at MS-based methods for studying higher order structures and biopolymers. A must for researchers in the field of biophysics, structural biology, and protein chemistry.

Introduction to Biophysical Methods for Protein and Nucleic Acid Research

Introduction to Biophysical Methods for Protein and Nucleic Acid Research
Author: Jay A. Glasel,Murray P. Deutscher
Publsiher: Academic Press
Total Pages: 510
Release: 1995-11-20
ISBN 10: 9780080534985
ISBN 13: 0080534988
Language: EN, FR, DE, ES & NL

Introduction to Biophysical Methods for Protein and Nucleic Acid Research Book Review:

The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment. Key Features * Modern Use of Mass Spectroscopy * NMR Spectroscopy * Molecular Modeling and Graphics * Macintosh and DOS/Windows 3.x disks

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals
Author: Damian J. Houde,Steven A. Berkowitz
Publsiher: Elsevier
Total Pages: 586
Release: 2019-11-13
ISBN 10: 0444641742
ISBN 13: 9780444641748
Language: EN, FR, DE, ES & NL

Biophysical Characterization of Proteins in Developing Biopharmaceuticals Book Review:

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical’s developability and the technical decision-making process needed when dealing with biophysical characterization data. Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development Highlights the capabilities and limitations of each technique Discusses the underlining science of each tool Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry

Biophysical Techniques in Photosynthesis

Biophysical Techniques in Photosynthesis
Author: Thijs Aartsma,Jörg Matysik
Publsiher: Springer Science & Business Media
Total Pages: 520
Release: 2008-02-01
ISBN 10: 1402082509
ISBN 13: 9781402082504
Language: EN, FR, DE, ES & NL

Biophysical Techniques in Photosynthesis Book Review:

Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.

Biophysical Techniques in Drug Discovery

Biophysical Techniques in Drug Discovery
Author: Angeles Canales
Publsiher: Royal Society of Chemistry
Total Pages: 320
Release: 2017-11-14
ISBN 10: 1788012860
ISBN 13: 9781788012867
Language: EN, FR, DE, ES & NL

Biophysical Techniques in Drug Discovery Book Review:

Biophysical techniques are used in many key stages of the drug discovery process including in screening for new receptor ligands, in characterising drug mechanisms, and in validating data from biochemical and cellular assays. This book provides an overview of the biophysical methods applied in drug discovery today, including traditional techniques and newer developments. Perspectives from academia and industry across a spectrum of techniques are brought together in a single volume. Small and biotherapeutic approaches are covered and strengths and limitations of each technique are presented. Case studies illustrate the application of each technique in real applied examples. Finally, the book covers recent developments in areas such as electron microscopy with discussions of their possible impact on future drug discovery. This is a go-to volume for biophysicists, analytical chemists and medicinal chemists providing a broad overview of techniques of contemporary interest in drug discovery.

Development and Application of Mass Spectrometry based Biophysical Approaches

Development and Application of Mass Spectrometry based Biophysical Approaches
Author: Ying Zhang
Publsiher: Unknown
Total Pages: 492
Release: 2015
ISBN 10: 1928374650XXX
ISBN 13: OCLC:920875376
Language: EN, FR, DE, ES & NL

Development and Application of Mass Spectrometry based Biophysical Approaches Book Review:

Mass spectrometry (MS)-based biophysical approaches are new "tools" for protein characterization owing to its capability to analyze proteins and protein complexes that range in molecular weight from kDa to MDa. Protein characterization requires more than identifying the primary structure. More importantly, protein high order structures (i.e., secondary, tertiary and quaternary structures) are needed for biological studies. MS has become the major tool in studies of protein primary structure and post translational modifications (PTMs) over the past two decades. Because MS has high sensitivity and fast turnaround, more and more biophysical approaches rely on MS to generate information for protein higher order structures. One of the emerging biophysical approaches is MS-based protein footprinting. Protein surface regions can be covalently labeled by chemical reagents in a biologically relevant environment. These chemical labels can be read out by MS through either bottom-up or top-down MS proteomics analysis. The outcome provides protein conformational information. Among various chemical labeling strategies, hydrogen deuterium exchange (HDX) is one of the most commonly used approaches in MS-based protein biophysical studies. HDX-MS is introduced in Chapter 1 by covering the early developments and new applications especially in measuring interaction affinities. Although HDX-MS has been developed for decades, there are still many challenges in protein characterization that require new or improved HDX method development. One such challenge is characterization of protein aggregation. Protein aggregation leads to loss of protein function, and protein aggregates are implicated in several neurodegenerative diseases like Alzheimer's and Parkinson's diseases. A key issue in studies of protein aggregation is real-time monitoring under biologically relevant condition. We developed an HDX-MS-based approach by studying Alzheimer's disease related A[beta] aggregation, and we described this development in Chapter 2. A[beta] proteins are labeled by deuterium in a pulsed way during A[beta] aggregation. The extents of aggregations are monitored by MS as deuterium uptake. This pulsed HDX platform provides peptide-level information about A[beta] aggregation. Ligands (drug candidates) were also evaluated with this platform to determine how the drug candidates affect oligomerization (Chapter 3). Ligand interactions can induce protein conformational changes, which are required in various protein functions like signaling, enzyme activity. Such interactions are fundamental to all biological processes. One of the often used ligands in cells is calcium. Calcium interacts with a variety of calcium-binding proteins, most of which have conserved sequence that form EF-hand motifs to bind calcium. MS-HDX has been an important tool in studies of these typical calcium-binding proteins. Many proteins without an EF-hand motif also require calcium for their function. For example, protein-arginine deiminase (PAD) is an enzyme for arginine citrullination and binds calcium without EF-hand motif. We conducted differential HDX studies on PAD2 protein (Chapter 4). Multiple and cooperative calcium binding of PAD2 are detected by HDX. HDX was further extended by applying protein-ligand titration in an HDX experiment (i.e., Protein-ligand interactions by mass spectrometry, titration and H/D exchange, PLIMSTEX). The calcium binding affinity of each binding site can be elucidated by PLIMSTEX (Chapter 5). Protein aggregation or ligand-binding induced conformational changes can also be detected by MS-HDX. One significant question in MS-based biophysical studies is how to generate structural information for proteins in the absence of a high resolution structure. In a newly developed platform, we combined a traditional structural biology approach, homology modeling, and MS-HDX to generate a structural model for diheme cytochrome c (DHCC) from Heliobacterium (Chapter 6), a protein for which solvent accessibility information from HDX experiment was used as the guide for homology modeling and used to generate a refined structural model of DHCC by using various computational approaches. In summary, we describe in this thesis development and application of several new, refined approaches of HDX and analyze protein aggregation, protein-ligand binding and unknown protein structures. We hope other scientists can apply these approaches to solve complicated and demanding biological problems that are difficult to investigate using traditional biophysical methods.

Biophysics

Biophysics
Author: Mark C. Leake
Publsiher: CRC Press
Total Pages: 390
Release: 2016-09-15
ISBN 10: 1498702511
ISBN 13: 9781498702515
Language: EN, FR, DE, ES & NL

Biophysics Book Review:

An Up-to-Date Toolbox for Probing Biology Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs. The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes students from the physical sciences with essential biological knowledge. The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism. Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions. It concludes with a discussion of the future of this exciting field. Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.

Methods in Molecular Biophysics

Methods in Molecular Biophysics
Author: Igor N. Serdyuk,Nathan R. Zaccai,Joseph Zaccai,Giuseppe Zaccai
Publsiher: Cambridge University Press
Total Pages: 684
Release: 2017-05-18
ISBN 10: 1107056373
ISBN 13: 9781107056374
Language: EN, FR, DE, ES & NL

Methods in Molecular Biophysics Book Review:

A comprehensive graduate textbook explaining key physical methods in biology, reflecting the very latest research in this fast-moving field.

Methods in Modern Biophysics

Methods in Modern Biophysics
Author: Bengt Nölting
Publsiher: Springer Science & Business Media
Total Pages: 254
Release: 2013-03-09
ISBN 10: 3662053675
ISBN 13: 9783662053676
Language: EN, FR, DE, ES & NL

Methods in Modern Biophysics Book Review:

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

Proteomics for Biological Discovery

Proteomics for Biological Discovery
Author: Timothy D. Veenstra,John R. Yates
Publsiher: John Wiley & Sons
Total Pages: 320
Release: 2006-06-12
ISBN 10: 0470007737
ISBN 13: 9780470007730
Language: EN, FR, DE, ES & NL

Proteomics for Biological Discovery Book Review:

Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.

Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen Exchange Mass Spectrometry of Proteins
Author: David D. Weis
Publsiher: John Wiley & Sons
Total Pages: 376
Release: 2016-01-12
ISBN 10: 1118703731
ISBN 13: 9781118703731
Language: EN, FR, DE, ES & NL

Hydrogen Exchange Mass Spectrometry of Proteins Book Review:

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Biophysical Mass Spectrometry Techniques for Probing the Higher Order Structure of Proteins and Complexes

Biophysical Mass Spectrometry Techniques for Probing the Higher Order Structure of Proteins and Complexes
Author: Sterling, Jr. (Harry John)
Publsiher: Unknown
Total Pages: 366
Release: 2012
ISBN 10: 1928374650XXX
ISBN 13: OCLC:810064697
Language: EN, FR, DE, ES & NL

Biophysical Mass Spectrometry Techniques for Probing the Higher Order Structure of Proteins and Complexes Book Review:

Electrospray ionization mass spectrometry (ESI-MS) is a powerful analytical platform for answering a wide variety of questions about the identity, quantity, structure, function, dynamics and energetics of biological molecules. Key advantages of ESI-MS include unrivaled specificity, attomole sensitivity, and the capacity for simultaneous analysis of complex mixtures with analyte masses that differ by less than 1 ppm. The low flow rates and sub-micron sized droplets formed with "nano" ESI allows biomolecular ions to be readily formed from purely aqueous or buffered aqueous solutions, and these ions have been shown to retain a "memory" of their solution-phase structures so that higher-order structural information can be obtained directly from a gas-phase measurement. All of the work described in this dissertation was undertaken in an effort to develop new nanoESI-based techniques that augment the existing array of biophysical mass spectrometry techniques for probing the structure/function relationships of biological molecules in their native environments. In part one, a hypothesis for the origin of nanoESI "supercharging" is developed and exhaustively tested utilizing a variety of solution- and gas-phase techniques with a range of different proteins and protein complexes. The results of all of these studies support the hypothesis that the origin of aqueous solution supercharging is the rapid chemical and/or thermal denaturation of a protein or protein complex analyte in an evaporating ESI droplet due to enrichment of the reagent caused by its high boiling point relative to that of water. Aqueous solution supercharging has recently been used in a variety of new applications and an understanding of its underlying mechanism is therefore essential. In part two, two new biophysical mass spectrometry applications are described. The first is a tandem-MS application of aqueous solution supercharging for obtaining hydrogen/deuterium exchange (HDX) rate constants in real-time with nearly single amino acid spatial resolution, and the second describes an MS method to obtain the quaternary structure of protein complexes that require high concentrations of essential salts. Finally, two ideas for new HDX-MS methods that capitalize on the mechanism of aqueous solution supercharging are outlined.

Applied Biophysics for Drug Discovery

Applied Biophysics for Drug Discovery
Author: Donald Huddler,Edward R. Zartler
Publsiher: John Wiley & Sons
Total Pages: 312
Release: 2017-10-02
ISBN 10: 111909948X
ISBN 13: 9781119099482
Language: EN, FR, DE, ES & NL

Applied Biophysics for Drug Discovery Book Review:

13.2.1 Protein Dynamics of SA WT and S1 Mutant DHFR in Apo and Bound States

Methods in Modern Biophysics

Methods in Modern Biophysics
Author: Bengt Nölting
Publsiher: Springer Science & Business Media
Total Pages: 273
Release: 2009-10-05
ISBN 10: 3642030211
ISBN 13: 9783642030215
Language: EN, FR, DE, ES & NL

Methods in Modern Biophysics Book Review:

Incorporating recent dramatic advances, this textbook presents a fresh and timely introduction to modern biophysical methods. It presents novel information, and is the first book to survey and explain the ten key biophysical methods.

Biophysical Methods for Biotherapeutics

Biophysical Methods for Biotherapeutics
Author: Tapan K. Das
Publsiher: Wiley
Total Pages: 376
Release: 2014-04-28
ISBN 10: 0470938439
ISBN 13: 9780470938430
Language: EN, FR, DE, ES & NL

Biophysical Methods for Biotherapeutics Book Review:

With a focus on practical applications of biophysical techniques, this book links fundamental biophysics to the process of biopharmaceutical development. • Helps formulation and analytical scientists in pharma and biotech better understand and use biophysical methods • Chapters organized according to the sequential nature of the drug development process • Helps formulation, analytical, and bioanalytical scientists in pharma and biotech better understand and usestrengths and limitations of biophysical methods • Explains how to use biophysical methods, the information obtained, and what needs to be presented in a regulatory filing, assess impact on quality and immunogenicity • With a focus on practical applications of biophysical techniques, this book links fundamental biophysics to the process of biopharmaceutical development.

Applied Biophysics for Drug Discovery

Applied Biophysics for Drug Discovery
Author: Donald Huddler,Edward R. Zartler
Publsiher: John Wiley & Sons
Total Pages: 312
Release: 2017-07-14
ISBN 10: 1119099501
ISBN 13: 9781119099505
Language: EN, FR, DE, ES & NL

Applied Biophysics for Drug Discovery Book Review:

Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.

Single Molecule Cellular Biophysics

Single Molecule Cellular Biophysics
Author: Mark C. Leake
Publsiher: Cambridge University Press
Total Pages: 284
Release: 2013-01-31
ISBN 10: 1107005833
ISBN 13: 9781107005839
Language: EN, FR, DE, ES & NL

Single Molecule Cellular Biophysics Book Review:

Indispensable textbook for undergraduate students in the physical and life sciences, unravelling the inner workings of the cell.

Proteomics and Protein Protein Interactions

Proteomics and Protein Protein Interactions
Author: Gabriel Waksman
Publsiher: Springer Science & Business Media
Total Pages: 323
Release: 2005-12-21
ISBN 10: 9780387245317
ISBN 13: 0387245316
Language: EN, FR, DE, ES & NL

Proteomics and Protein Protein Interactions Book Review:

The rapidly evolving field of protein science has now come to realize the ubiquity and importance of protein-protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of high throughput proteomics to monitor protein-protein interactions at an organism level, we can now safely state that protein-protein interactions are the norm and not the exception. Thus, protein function must be understood in the larger context of the various binding complexes that each protein may form with interacting partners at a given time in the life cycle of a cell. Proteins are now seen as forming sophisticated interaction networks subject to remarkable regulation. The study of these interaction networks and regulatory mechanism, which I would like to term "systems proteomics," is one of the thriving fields of proteomics. The bird-eye view that systems proteomics offers should not however mask the fact that proteins are each characterized by a unique set of physical and chemical properties. In other words, no protein looks and behaves like another. This complicates enormously the design of high-throughput proteomics methods. Unlike genes, which, by and large, display similar physico-chemical behaviors and thus can be easily used in a high throughput mode, proteins are not easily amenable to the same treatment. It is thus important to remind researchers active in the proteomics field the fundamental basis of protein chemistry. This book attempts to bridge the two extreme ends of protein science: on one end, systems proteomics, which describes, at a system level, the intricate connection network that proteins form in a cell, and on the other end, protein chemistry and biophysics, which describe the molecular properties of individual proteins and the structural and thermodynamic basis of their interactions within the network. Bridging the two ends of the spectrum is bioinformatics and computational chemistry. Large data sets created by systems proteomics need to be mined for meaningful information, methods need to be designed and implemented to improve experimental designs, extract signal over noise, and reject artifacts, and predictive methods need to be worked out and put to the test. Computational chemistry faces similar challenges. The prediction of binding thermodynamics of protein-protein interaction is still in its infancy. Proteins are large objects, and simplifying assumptions and shortcuts still need to be applied to make simulations manageable, and this despite exponential progress in computer technology. Finally, the study of proteins impacts directly on human health. It is an obvious statement to say that, for decades, enzymes, receptors, and key regulator proteins have been targeted for drug discovery. However, a recent and exciting development is the exploitation of our knowledge of protein-protein interaction for the design of new pharmaceuticals. This presents particular challenges because protein-protein interfaces are generally shallow and interactions are weak. However, progress is clearly being made and the book seeks to provide examples of successes in this area.

Progress in Biophysics and Biophysical Chemistry

Progress in Biophysics and Biophysical Chemistry
Author: J. A. V. Butler,B. Katz
Publsiher: Elsevier
Total Pages: 370
Release: 2016-06-06
ISBN 10: 1483157032
ISBN 13: 9781483157030
Language: EN, FR, DE, ES & NL

Progress in Biophysics and Biophysical Chemistry Book Review:

Progress in Biophysics and Biophysical Chemistry, Volume 7 focuses on the applications of physical or physicochemical ideas and methods to biological problems, including the use of isotopes to investigate metabolic processes. Other subjects discussed in detail are the electric organs of fishes; the thermodynamics of agglutination of red cells; muscle structure and function; and the structure of bone. This book is comprised of seven chapters and begins with a review of the mechanisms of discharge of electric organs in fish in the contexts of general and comparative electrophysiology, paying particular attention to synaptic excitability and the involvement of several electrogenic components in the reflex discharge. The evolution of electric organs in fish is also discussed. The following chapters explore the thermodynamics of isohemagglutinins; use of labeled plasma proteins to study nutritional problems; use of isotopes to analyze intermediary metabolism; and X-crystal analysis of bone. The final two chapters are devoted to muscle structure and theories of contraction, chloroplast structure, and energy conversion in photosynthesis. This volume will be of interest to biophysicists, physicists, and physical chemists working with biological materials.