Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology
Author: Stefan Surzycki
Publsiher: Springer Science & Business Media
Total Pages: 434
Release: 2012-12-06
ISBN 10: 3642569684
ISBN 13: 9783642569685
Language: EN, FR, DE, ES & NL

Basic Techniques in Molecular Biology Book Review:

This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Basic Techniques in Biochemistry and Molecular Biology

Basic Techniques in Biochemistry and Molecular Biology
Author: R. K. Sharma
Publsiher: I. K. International Pvt Ltd
Total Pages: 280
Release: 2008-11-15
ISBN 10: 819074626X
ISBN 13: 9788190746267
Language: EN, FR, DE, ES & NL

Basic Techniques in Biochemistry and Molecular Biology Book Review:

Fundamentals of biochemistry and molecular biology is an important component of all disciplines of Biology. In the era of multidisciplinary approach, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discussed all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites and molecular biology. It includes a wide range of experiments from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been taken to the safety in laboratory and precautions are given wheresoever required.

Molecular Biology Techniques

Molecular Biology Techniques
Author: Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
Publsiher: Academic Press
Total Pages: 200
Release: 2012
ISBN 10: 0123855446
ISBN 13: 9780123855442
Language: EN, FR, DE, ES & NL

Molecular Biology Techniques Book Review:

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Basic Techniques of Molecular Biology

Basic Techniques of Molecular Biology
Author: Tyshawn Moore
Publsiher: Unknown
Total Pages: 329
Release: 2018
ISBN 10: 9781684694914
ISBN 13: 1684694914
Language: EN, FR, DE, ES & NL

Basic Techniques of Molecular Biology Book Review:

Basic Techniques in Biochemistry Microbiology and Molecular Biology

Basic Techniques in Biochemistry  Microbiology and Molecular Biology
Author: Aakanchha Jain,Richa Jain,Sourabh Jain
Publsiher: Humana
Total Pages: 334
Release: 2020-01-24
ISBN 10: 9781493998609
ISBN 13: 1493998609
Language: EN, FR, DE, ES & NL

Basic Techniques in Biochemistry Microbiology and Molecular Biology Book Review:

This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.

Basic Methods in Molecular Biology

Basic Methods in Molecular Biology
Author: Leonard Davis
Publsiher: Elsevier
Total Pages: 399
Release: 2012-12-02
ISBN 10: 044460149X
ISBN 13: 9780444601490
Language: EN, FR, DE, ES & NL

Basic Methods in Molecular Biology Book Review:

Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
Author: Keith Wilson,John Walker
Publsiher: Cambridge University Press
Total Pages: 329
Release: 2010-03-04
ISBN 10: 1107393493
ISBN 13: 9781107393493
Language: EN, FR, DE, ES & NL

Principles and Techniques of Biochemistry and Molecular Biology Book Review:

This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Techniques in Molecular Biology

Techniques in Molecular Biology
Author: J.M. Walker,W. Gaastra
Publsiher: Springer Science & Business Media
Total Pages: 335
Release: 2012-12-06
ISBN 10: 9401165637
ISBN 13: 9789401165631
Language: EN, FR, DE, ES & NL

Techniques in Molecular Biology Book Review:

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology
Author: Andreas Hofmann,Samuel Clokie
Publsiher: Cambridge University Press
Total Pages: 962
Release: 2018-03-31
ISBN 10: 1107162270
ISBN 13: 9781107162273
Language: EN, FR, DE, ES & NL

Wilson and Walker s Principles and Techniques of Biochemistry and Molecular Biology Book Review:

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Molecular Biology Techniques

Molecular Biology Techniques
Author: Walt Ream,Katharine G. Field
Publsiher: Academic Press
Total Pages: 234
Release: 1998-11-17
ISBN 10: 0080536824
ISBN 13: 9780080536828
Language: EN, FR, DE, ES & NL

Molecular Biology Techniques Book Review:

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
Author: Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publsiher: Academic Press
Total Pages: 218
Release: 2020-11-10
ISBN 10: 012824450X
ISBN 13: 9780128244500
Language: EN, FR, DE, ES & NL

Advanced Methods in Molecular Biology and Biotechnology Book Review:

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Plant Molecular Biology

Plant Molecular Biology
Author: P. G. Jones,J. M. Sutton
Publsiher: John Wiley & Son Limited
Total Pages: 213
Release: 1997-10-09
ISBN 10:
ISBN 13: UOM:39015039881936
Language: EN, FR, DE, ES & NL

Plant Molecular Biology Book Review:

The authors describe the most important currently used protocols in plant molecular biology. They give useful tips on stopping points and troubleshooting as well as providing information on safety. Lists of suppliers are included.

Techniques for Molecular Biology

Techniques for Molecular Biology
Author: D Tagu,C Moussard
Publsiher: Science Publishers
Total Pages: 227
Release: 2006-01-04
ISBN 10: 9781578083619
ISBN 13: 1578083613
Language: EN, FR, DE, ES & NL

Techniques for Molecular Biology Book Review:

With the underpinning role of forage legumes in the nitrogen economy and animal productivity from temperate grasslands certain to expand in the future, particularly in regions where their potential has not yet been realized, it is essential that the wealth of information currently available is widely disseminated. This book serves the purpose with very detailed information on and illustrations of 35 selected forage legume species that will contribute to more efficient and viable grassland farming.

Morphology Methods

Morphology Methods
Author: Ricardo V. Lloyd
Publsiher: Springer Science & Business Media
Total Pages: 422
Release: 2001-06-15
ISBN 10: 1592591906
ISBN 13: 9781592591909
Language: EN, FR, DE, ES & NL

Morphology Methods Book Review:

The past several decades have witnessed an impressive array of conceptual and techno logical advances in the biomedical sciences. Much of the progress in this area has developed directly as a result of new morphology-based methods that have permitted the assessment of chemical, enzymatic, immunological, and molecular parameters at the cellular and tissue levels. Additional novel approaches including laser capture microdissection have also emerged for the acquisition of homogeneous cell popula tions for molecular analyses. These methodologies have literally reshaped the approaches to fundamental biological questions and have also had a major impact in the area of diagnostic pathology. Much of the groundwork for the development of morphological methods was estab th lished in the early part of the 19 century by Francois-Vincent Raspail, generally acknowledged as the founder of the science of histochemistry. The earliest work in the field was primarily in the hands of botanists and many of the approaches to the under standing of the chemical composition of cells and tissues involved techniques such as microincineration, which destroyed structural integrity. The development of aniline th dyes in the early 20 century served as a major impetus to studies of the structural rather than chemical composition of tissue. Later in the century, however, the focus returned to the identification of chemical constituents in the context of intact cell and tissue structure.

Diagnostic Molecular Biology

Diagnostic Molecular Biology
Author: Chang-Hui Shen
Publsiher: Academic Press
Total Pages: 471
Release: 2019-04-02
ISBN 10: 012802867X
ISBN 13: 9780128028674
Language: EN, FR, DE, ES & NL

Diagnostic Molecular Biology Book Review:

Diagnostic Molecular Biology describes the fundamentals of molecular biology in a clear, concise manner to aid in the comprehension of this complex subject. Each technique described in this book is explained within its conceptual framework to enhance understanding. The targeted approach covers the principles of molecular biology including the basic knowledge of nucleic acids, proteins, and genomes as well as the basic techniques and instrumentations that are often used in the field of molecular biology with detailed procedures and explanations. This book also covers the applications of the principles and techniques currently employed in the clinical laboratory. • Provides an understanding of which techniques are used in diagnosis at the molecular level • Explains the basic principles of molecular biology and their application in the clinical diagnosis of diseases • Places protocols in context with practical applications

Basic Molecular Biology

Basic Molecular Biology
Author: Philippa D. Darbre
Publsiher: Wiley-Blackwell
Total Pages: 208
Release: 1999-01-12
ISBN 10: 9780471977056
ISBN 13: 0471977055
Language: EN, FR, DE, ES & NL

Basic Molecular Biology Book Review:

For the increasing number of professionals needing to learn about and use molecular biology techniques in their research, the daunting challenge is to break through the technical and largely inaccessible jargon found in most texts. This unique introduction to the field makes things much easier. The author begins with a brief overview of the basics, then focuses on simple step-by-step protocols of the core techniques, giving a theoretical outline, valuable advice on real-world problems readers might encounter, and estimates on how much time is required to perform each technique.

Methods in Plant Molecular Biology

Methods in Plant Molecular Biology
Author: Mary A. Schuler,Raymond E. Zielinski
Publsiher: Academic Press
Total Pages: 171
Release: 2012-12-02
ISBN 10: 0323137652
ISBN 13: 9780323137652
Language: EN, FR, DE, ES & NL

Methods in Plant Molecular Biology Book Review:

Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. Eight basic experiments which can be used collectively or individually cover Recombinant Cloning and Screening in E. coli; DNA Sequencing Plant RNA Isolation and in Vitro Translations Plant DNA Isolations and Genomic DNA Southern Analysis Chloroplast Isolation and Protein Synthesis Plant Tissue Culture and Agrobacterium Transformations Experiments that have been student tested for three years Blueprints for setting up gel rigs Comprehensive course schedule outlining individual procedures to be finished in each lab segment Course can be tailored to suit the needs of the individual instructor

Advances in Molecular Techniques

Advances in Molecular Techniques
Author: Rakesh S. Sengar,Amit Kumar,Reshu Chaudhary,Ashu Singh
Publsiher: CRC Press
Total Pages: 436
Release: 2018-05-11
ISBN 10: 1351021206
ISBN 13: 9781351021203
Language: EN, FR, DE, ES & NL

Advances in Molecular Techniques Book Review:

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Analytical Techniques in Biochemistry and Molecular Biology

Analytical Techniques in Biochemistry and Molecular Biology
Author: Rajan Katoch
Publsiher: Springer Science & Business Media
Total Pages: 441
Release: 2011-07-19
ISBN 10: 9781441997852
ISBN 13: 1441997857
Language: EN, FR, DE, ES & NL

Analytical Techniques in Biochemistry and Molecular Biology Book Review:

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Molecular Biology

Molecular Biology
Author: David P. Clark
Publsiher: Elsevier
Total Pages: 784
Release: 2005-06-24
ISBN 10: 9780080454214
ISBN 13: 0080454216
Language: EN, FR, DE, ES & NL

Molecular Biology Book Review:

Molecular Biology: Academic Cell Update provides an introduction to the fundamental concepts of molecular biology and its applications. It deliberately covers a broad range of topics to show that molecular biology is applicable to human medicine and health, as well as veterinary medicine, evolution, agriculture, and other areas. The present Update includes the study guide with online content, journal specific images, and test bank. It also offers vocabulary flashcards and online self-quizzing called Test Prep. The book begins by defining some basic concepts in genetics such as biochemical pathways, phenotypes and genotypes, chromosomes, and alleles. It explains the characteristics of cells and organisms, DNA, RNA, and proteins. It also describes genetic processes such as transcription, recombination and repair, regulation, and mutations. The chapters on viruses and bacteria discuss their life cycle, diversity, reproduction, and gene transfer. Later chapters cover topics such as molecular evolution; the isolation, purification, detection, and hybridization of DNA; basic molecular cloning techniques; proteomics; and processes such as the polymerase chain reaction, DNA sequencing, and gene expression screening. *Now with an online study guide with the most current, relevant research from Cell Press *Full supplements including test bank, powerpoint and online self quizzing *Up to date description of genetic engineering, genomics, and related areas * Basic concepts followed by more detailed, specific applications * Hundreds of color illustrations enhance key topics and concepts * Covers medical, agricultural, and social aspects of molecular biology * Organized pedagogy includes running glossaries and keynotes (mini-summaries) to hasten comprehension