Optical Biosensors

Optical Biosensors, 2ed describes the principles of successful systems, examples of applications, and evaluates the advantages and deficiencies of each. It also addresses future developments on two levels: possible improvements in existing systems and emerging technologies that could provide new capabilities in the future. The book is formatted for ease of use and is therefore suitable for scientists and engineers, students and researcher at all levels in the field. * Comprehensive analysis and review of the underlying principles by optical biosensors * Updates and informs on all the latest developments and hot topic areas * Evaluates current methods showing the advantages and disadvantages of various systems involved

PART I. Optical Biosensors: The Present -- Chapter 1. Optrode-based Fiber Optic Biosensors -- Israel Biran and David R. Walt -- Chapter 2. Evanescent Wave Fiber Optic Biosensors -- Chris Rowe Taitt and Frances S. Ligler -- Chapter 3. Planar Waveguides for Fluorescence Biosensors -- Kim Sapsford, Chris Rowe Taitt, and Frances S. Ligler -- Chapter 4. Flow Immnosensor -- Anne W. Kusterbeck -- Chapter 5. Time Resolved Fluorescence -- Richard Thompson -- Chapter 6. Electrochemiluminescence -- Mark M. Richter -- Chapter 7. Surface Plasmon Resonance Biosensors -- Jiri Homola, Sinclair Yee, and David Myszka -- Chapter 8. The Resonant Mirror Optical Biosensor -- Tim Kinning and Paul Edwards -- Chapter 9. Interferometric Biosensors -- Daniel P. Campbell and Candice J. McCloskey -- Part II. Optical Biosensors: The Future -- Chapter 10. Genetic Engineering of Signaling Molecules -- Agatha Feltus and Sylvia Daunert -- Chapter 11. Artificial Receptors for Chemosensors -- Thomas W. Bell and Nicholas ...

This book introduces readers to the development of novel optical biosensors for environmental analysis. Environmental pollution has now become a serious problem, which threatens the health of human beings. Traditional analytical methods have a number of drawbacks, such as the need for professional operators and complicated instruments. After millions of years of evolution, biomolecules can perform various functions with good accuracy and efficiency due to their unique structures, offering a viable alternative to traditional methods. This work focuses on using new biological sensing strategies, e.g. those based on special biomaterials, bio-reactions or living cells, to establish novel biosensors. As these biosensors offer satisfactory optical response performance, they can be used to transform the recognition behavior of specific targets into optical signals and effectively detect target objects.

Biosensors and Modern Biospecific Analytical Techniques further expands the Comprehensive Analytical Chemistry series' coverage of rapid analysis based on advanced technological developments. This 12-chapter volume summarizes the main developments in the biosensors field over the last 10 years. It provides a comprehensive study on the different types of biosensors, including DNA-based, enzymatic, optical, self-assembled monolayers and the third generation of biosensors. As well as many technological developments on bioanalytical microsystems and new materials for biosensors, antibody and immunoassay developments have a prominent place in the book. * Provides a comprehensive study on the different types of biosensors * Applications covered include environmental analysis, bioprocess monitoring and biomedicine * An indispensable resource for those working in analytical chemistry

Commencing by defining a set of standards for benchmarking this book then proceeds to provide a review of the physics and workings of several optical and super optical biosensors, and introduces two more super optical biosensors. Mathcad is used to analyse these virtual biosensors and these codes and available to readers for use in their own research.

Nowadays, the implementation of novel technological platforms in biosensor-based developments is primarily directed to the miniaturization of analytical systems and lowering the limits of detection. Rapid scientific and technological progress enables the application of biosensors for the online detection of minute concentrations of different chemical compounds in a wide selection of matrixes and monitoring extremely low levels of biomarkers even in living organisms and individual cells. This book, including 16 chapters, characterizes the present state of the art and prospective options for micro and nanoscale activities in biosensors construction and applications.

The purpose of this thesis was to develop optical biosensors based on nanostructured porous materials; porous silicon (pSi) and porous anodized aluminum oxide (pAAO) to detect biomarkers found in chronic wound fluid (matrix metalloproteinases or MMPs,bacterial enzymes and glucose) in order to demonstrate their potential for POC devices.

This interesting book covers latest aspects of a highly sophisticated technology; results treated in critical detail; demonstrates applicability of this technology to practical problems in process control, biochip methods, clinical analysis, environmental sciences

For the first time, distinguished scientists from key institutions worldwide provide a comprehensive approach to optical sensing techniques employing the phenomenon of guided wave propagation for chemical and biosensors. This includes both state-of the-art fundamentals and innovative applications of these techniques. The authors present a deep analysis of their particular subjects in a way to address the needs of novice researchers such as graduate students and post-doctoral scholars as well as of established researchers seeking new avenues. Researchers and practitioners who need a solid foundation or reference will find this work invaluable. This second of two volumes covers the incorporation of periodic structures in waveguides to exploit the Bragg phenomenon, optical fiber sensors, hollow waveguides and micro-resonators as well as a review of the tremendous expansion of terahertz technology for sensing applications.

Nanomaterials for Biosensors: Fundamentals and Applications provides a detailed summary of the main nanomaterials used in biosensing and their application. It covers recent developments in nanomaterials for the fabrication of biosensor devices for healthcare diagnostics, food freshness and bioprocessing. The various processes used for synthesis and characterization of nanostructured materials are examined, along with the design and fabrication of bioelectronic devices using nanostructured materials as building blocks. Users will find the fundamentals of the main nanomaterials used in biosensing, helping them visualize a systematic and coherent picture of how nanomaterials are used in biosensors. The book also addresses the role of bio-conjugation of nanomaterials in the construction of nano-biointerfaces for application in biosensors. Such applications, including metal nanoparticles, metal oxide nanoparticles, nanocomposites, carbon nanotubes, conducting polymers and plasmonic nanostructures in biosensing are discussed relative to each nanomaterial concerned. Finally, recent advancements in protein functionalized nanomaterials for cancer diagnostics and bio-imaging are also included. Provides a detailed study on how nanomaterials are used to enhance sensing capabilities in biosensors Explains the properties, characterization methods and preparation techniques of the nanomaterials used in biosensing Arranged in a material-by-material way, making it clear how each nanomaterial should be used

New Insights on Optical Biosensors: Techniques, Construction and Application.

Comprehensive Biomaterials brings together the myriad facets of biomaterials into one, major series of six edited volumes that would cover the field of biomaterials in a major, extensive fashion: Volume 1: Metallic, Ceramic and Polymeric Biomaterials Volume 2: Biologically Inspired and Biomolecular Materials Volume 3: Methods of Analysis Volume 4: Biocompatibility, Surface Engineering, and Delivery Of Drugs, Genes and Other Molecules Volume 5: Tissue and Organ Engineering Volume 6: Biomaterials and Clinical Use Experts from around the world in hundreds of related biomaterials areas have contributed to this publication, resulting in a continuum of rich information appropriate for many audiences. The work addresses the current status of nearly all biomaterials in the field, their strengths and weaknesses, their future prospects, appropriate analytical methods and testing, device applications and performance, emerging candidate materials as competitors and disruptive technologies, and strategic insights for those entering and operational in diverse biomaterials applications, research and development, regulatory management, and commercial aspects. From the outset, the goal was to review materials in the context of medical devices and tissue properties, biocompatibility and surface analysis, tissue engineering and controlled release. It was also the intent both, to focus on material properties from the perspectives of therapeutic and diagnostic use, and to address questions relevant to state-of-the-art research endeavors. Reviews the current status of nearly all biomaterials in the field by analyzing their strengths and weaknesses, performance as well as future prospects Presents appropriate analytical methods and testing procedures in addition to potential device applications Provides strategic insights for those working on diverse application areas such as R&D, regulatory management, and commercial development

General introduction to biosensors and recognition receptors -- Biomarkers in health care -- The use of nanomaterials and microfluidics in medical diagnostics -- SPR-based biosensor technologies in disease detection and diagnostics -- Piezoelectric-based biosensor technologies in disease detection and diagnostics -- Electrochemical-based biosensor technologies in disease detection and diagnostics -- MEMS-based cell counting methods -- Lab-on-a-chip platforms for disease detection and diagnosis -- Applications of quantum dots in biosensors and diagnostics -- Applications of molecularly imprinted nanostructures in biosensors and diagnostics -- Smart nanomaterial's : applications in biosensors and diagnostics -- Applications of magnetic nanomaterial's in biosensors and diagnostics -- Graphene applications in biosensors and diagnostics -- Optical biosensors and applications to drug discovery for cancer cases -- Biosensors for detection of anticancer drug-DNA interactions

This book covers optical chemical sensing by means of optical waveguides, from the fundamentals to the most recent applications. The book includes a historical review of the development of these sensors, from the earliest laboratory prototypes to the first commercial instrumentations. The book reprints a lecture by the Nobel Laureate Charles Townes on the birth of maser and laser, which lucidly illustrates the development of new science and new technology.

Of all things natural, light is the most sublime. From the very existential belief of the origins of the universe to its role in the evolution of life on earth, light has been inextricably woven into every aspect of our lives. I am grateful to Springer-Verlag and Thomas Scheper for this invitation to organize this volume that continues to expand the use of light to create next generation sensing applications. Indeed, the very act of expanding the frontiers of learning and knowledge are referred to in many languages and cultures as enlightenment. Early optical instruments relied largely on simple combinations of mirrors, prisms and lenses. With these simple devices, substantial progress was made in our understanding of the properties of light and of its interactions with matter. Things got more complicated with the evolution of optical instruments in labo- tory use. Early systems used bulky and expensive hardware to generate light, split it into the desired wavelengths and finally collect it for analysis. The discovery of the laser pushed the technology further, but did not do much to make its adoption more widespread as the lasers themselves were large and required substantial el- trical power to operate. The true revolution is just beginning. Advances in mic- electronics have resulted in the possibility of truly low-cost (using the consumer electronics industry as a parallel) devices that exploit optical measurement technology.

Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14th MPSA meeting. Major topics include: X-ray crystallography, mass spectrometry or cryo-electron microscopy tomography and different experimental approaches for the study of very large multi-subunit molecular nanomaschines; development of high throughput methods for large-scale protein expression and purification and automatic data acquisition for structure determination by both X-ray diffraction and NMR spectroscopy; mechanisms of protein folding and misfolding in vitro and in vivo; protein-protein interactions; analysis of post-translational modifications; the classification, prediction of structure or functional sites, and evolution of protein folds and functions. TOC: Includes 25 chapters organized in the following parts: Structural Proteomics Proteome Analysis Structure-Function Correlations Protein-Protein Interaction Advanced Technologies Protein Sequencing and Amino Acids Analysis Bioinformatics

Effect of Reynolds number on fractal binding kinetics on a surface-based biosensor -- DNA fractal binding and dissociation kinetics -- Fractal analysis of binding and dissociation interactions of estrogen receptors to ligands on biosensor surfaces -- A fractal analysis of analyte-estrogen receptor binding and dissociation kinetics using biosensors : environmental effects -- A fractal analysis of analyte-estrogen receptor binding and dissociation kinetics using biosensors : biomedical effects -- Fractal analysis of binding interactions of nuclear estrogen receptors occurring on biosensor surfaces -- A kinetic study of analyte-receptor binding and dissociation for biosensor applications : a fractal analysis for cholera toxin and peptide-protein interactions / -- The temporal nature of the binding and dissociation rate coefficients and the affinity values for biosensor kinetics -- Fractal analysis of analyte-receptor binding and dissociation, and dissociation alone for biosensor applicati ...

Light on physics and math, with a heavy focus on practical applications, Optical, Acoustic, Magnetic, and Mechanical Sensor Technologies discusses the developments necessary to realize the growth of truly integrated sensors for use in physical, biological, optical, and chemical sensing, as well as future micro- and nanotechnologies. Used to pick up sound, movement, and optical or magnetic signals, portable and lightweight sensors are perpetually in demand in consumer electronics, biomedical engineering, military applications, and a wide range of other sectors. However, despite extensive existing developments in computing and communications for integrated microsystems, we are only just now seeing real transformational changes in sensors, which are critical to conducting so many advanced, integrated tasks. This book is designed in two sections—Optical and Acoustic Sensors and Magnetic and Mechanical Sensors—that address the latest developments in sensors. The first part covers: Optical and acoustic sensors, particularly those based on polymer optical fibers Potential of integrated optical biosensors and silicon photonics Luminescent thermometry and solar cell analyses Description of research from United States Army Research Laboratory on sensing applications using photoacoustic spectroscopy Advances in the design of underwater acoustic modems The second discusses: Magnetic and mechanical sensors, starting with coverage of magnetic field scanning Some contributors’ personal accomplishments in combining MEMS and CMOS technologies for artificial microsystems used to sense airflow, temperature, and humidity MEMS-based micro hot-plate devices Vibration energy harvesting with piezoelectric MEMS Self-powered wireless sensing As sensors inevitably become omnipresent elements in most aspects of everyday life, this book assesses their massive potential in the development of interfacing applications for various areas of product design and sciences—including electronics, photonics, mechanics, chemistry, and biology, to name just a few.