Microbial Extremozymes

1. Introduction, 2. Fundamentals of Microbiology, 3. Proteins-An overview, 4. Enzymes-General Perspective, 5. Immobilized Enzymes and Microbial Whole cell Technology, 6. Nucleic Acids-Structure and Functions, 7. Genetic Engineering, 8. Submerged Culture Fermentation, 9. Solid-State Fermentation, 10. Downstream Processing, 11. Enzyme Technology-Medical Applications, 12. Enzyme Technology-Industrial Applications, 13. Constituents of Skins-Their Role in Leather Processing, 14. Microbial Control in Curing Process, 15. Enzymes in Soaking, 16. Dehairing-Conventional and Enzymatic Methods, 17. Bating-State of Art, 18. Degreasing-Analysis of Different Sysytem, 19. Recent Trends in Waste Management, 20. Protocols for Enzyme Evaluation, 21. What is Ahead.

This book explores microbial lifestyles, biochemical adaptations, and trophic interactions occurring in extreme environments. By summarizing the latest findings in the field it provides a valuable reference for future studies. Spark ideas for biotechnological and commercial exploitation of microbiomes at the extremes of life are presented. Chapters on viruses complement this highly informative book. In a vertical journey through the microbial biosphere it covers aspects of cold environments, hot environments, extreme saline environments, and extreme pressure environments, and more. From the deep sea, through polar deserts, up to the clouds in the air - the diversity of microbial life in all habitats is described, explored, and comprehensively reviewed. Possible biotechnical applications are discussed. This book aims to provide a useful reference for those who want to start a research program in extreme microbiology and, hopefully, inspire new research directions.

The aim of food processing is to produce food that is palatable and tastes good, extend its shelf-life, increase the variety, and maintain the nutritional and healthcare quality of food. To achieve favorable processing conditions and for the safety of the food to be consumed, use of food grade microbial enzymes or microbes (being the natural biocatalysts) is imperative. This book discusses the uses of enzymes in conventional and non-conventional food and beverage processing as well as in dairy processing, brewing, bakery and wine making. Apart from conventional uses, the development of bioprocessing tools and techniques have significantly expanded the potential for extensive application of enzymes such as in production of bioactive peptides, oligosaccharides and lipids, flavor and colorants. Some of these developments include extended use of the biocatalysts (as immobilized/encapsulated enzymes), microbes (both natural and genetically modified) as sources for bulk enzymes, solid state fermentation technology for enzyme production. Extremophiles and marine microorganisms are another source of food grade enzymes. The book throws light on potential applications of microbial enzymes to expand the base of food processing industries.

New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Cellulase System Properties and Applications covers the biochemistry of cellulase system, its mechanisms of action, and its industrial applications. Research has shed new light on the mechanisms of microbial cellulase production and has led to the development of technologies for production and applications of cellulose degrading enzymes. The biological aspects of processing of cellulosic biomass have become the crux of future research involving cellulases and cellulolytic microorganisms, as they are being commercially produced by several industries globally and are widely being used in food, animal feed, fermentation, agriculture, pulp and paper, and textile applications. The book discusses modern biotechnology tools, especially in the area of microbial genetics, novel enzymes, and new enzyme and the applications in various industries. As a professional reference, this new book is useful to all researchers working with microbial cellulase system, both academic institutions and industry-based research bodies, as well as to teachers, graduate, and postgraduate students with information on continuous developments in microbial cellulase system. The book provides an indispensable reference source for chemists, biochemical engineers/bioengineers, biochemists, biotechnologists and researchers who want to know about the unique properties of this microbe and explore its future applications. Compiles the latest developments made and currently undergoing in the area of microbial cellulase system Chapters are contributed from top researchers on this area around the globe Includes information related to almost all areas of microbial cellulase system Extensive cover of current industrial applications and discusses potential future applications

Microbial enzymes play a vital role in maintaining soil health and removing pollutants from contaminated land. Soil microflora is closely associated with maintaining soil fertility, and the use of chemical pesticides, fertilizers and other volatile sprays in agriculture threatens the health ofthe microbial population in the soil. Every single particle of healthy soil contains millions of bacteria, which interact with the nutrients available, sustaining the nutrient cycle and making this microflora an essential component of life on earth. How do microbes help in the nutrient cycle? Either by intracellular digestion of macromolecules and converting these into smaller units in their metabolic pathways, or by secreting enzymes into the extracellular environment to facilitate the conversion of complex macromolecules into micro-molecules that can be easily absorbed by other living species. To meet demands for energy and food for the growing global population, it is important to protect agricultural land from contamination and maintain its productivity. Heavy metal ions from contaminated land canenter crops, fish or aquatic organismsvia contaminated water, and theseare then taken up by the human body, where they can accumulate and alter the normal microflora. The microbiological component of the soil is ahighly complex system and is still not fully understood. How do microbes survive in the changing physicochemical environment of soil?. This book helps readers understand the mechanism, various routes of microbialsoil remediation, the interactionsof different genera, and how microbial enzymes support the sustainable restoration of healthy soil.

This book introduces fundamentals of enzymatic processes, various renewable energy resources and their pretreatment processes. It presents in-depth review of extremophilic enzymes (e.g., Cellulases, Xylanases, Lytic Polysaccharide Monooxygenases, Amylases, Ligninases, Pectinases, Esterases, and Chitinases) which can be used in several biotechnological processes. In addition, the authors present expert knowledge on how to engineer enzymes for enhanced conversion of lignocellulosic feedstocks to biofuels.Extremozymes play important roles in many kinds of bioprocessing e.g., in conversion of non-food biomass into usable power. Existing enzymatic technologies, including hydrolysis of lignocellulose into sugars, have several limitations such as they have very slow enzymatic hydrolysis rates, yields low products, requires high dosages of enzymes, and are sensitive to microbial contamination problems. These limitations could be overcome using extremophilic enzymes.

Handbook of Indigenous Foods Involving Alkaline Fermentation details the basic approaches of alkaline fermentation, provides a brief history, and offers an overview of the subject. Devoted exclusively to alkaline-fermented foods (AFFs), this text includes contributions from experts from around the globe. It discusses the diversity of indigenous fer

Physiological and Biotechnological Aspects of Extremophiles highlights the current and topical areas of research in this rapidly growing field. Expert authors from around the world provide the latest insights into the mechanisms of these fascinating organisms use to survive. The vast majority of extremophiles are microbes which include archaea, bacteria and some eukaryotes. These microbes live under chemical and physical extremes that are usually lethal to cellular molecules, yet they manage to survive and even thrive. Extremophiles have important practical uses. They are a valuable source of industrially important enzymes and recent research has revealed novel mechanisms and biomolecular structures with a broad range of potential applications in biotechnology, biomining, and bioremediation. Aimed at research scientists, students, microbiologists, and biotechnologists, this book is an essential reading for scientists working with extremophiles and a recommended reference text for anyone interested in the microbiology, bioprospecting, biomining, biofuels, and extremozymes of these organisms. Shows the implications of the physiological adaptations of microbes from extreme habitats that are largely contributed by their biomolecules from basic to applied research Provides in-depth knowledge of genomic plasticity and proteome of different extremophiles Gives detailed and comprehensive insight about use of genetic engineering as well as genome editing for industrial applications

Diversity perspectives are discussed in the context of ecosystem dynamics, taking into consideration environments that are rather unique to microorganisms. Considerable thrust is placed on the role that microorganisms play in sustainable production systems. Microbe-plant interaction arena is highlighted through the discussion of mycorrhizal partners, on which depends not only the plant community structure but also abatement of abiotic and biotic stresses. Other mutualist, rhizobia gets its due coverage whereas plant disease component carries examples from both, fungal and viral disease point of view. Considerable emphasis is placed on a discussion of the environmental issues such as the approaches that will lead to newer bioremediation technologies. No discussion of microbial diversity is complete without their implications in animal and human health. Discussed in this context are L-arginases in cancer therapy as also bioactives from cyanobacteria. Genomics and pathogenicity of two groups of viruses, viz., blue tongue and flaviviruses is highlighted whereas keratinophilic fungal forms are discussed in the context of dermatophytic infections. This volume also carries a fair number of articles on commercial microbiology.

The book is an excellent compilation of chapters on fruitful applications of Biotechnology. The chapters have been authored by eminent scholars from India and abroad working on diverse disciplines related to Biotechnology. The book is an invaluable source of information on biosensors, microbial surfactants, enzyme immobilization, disease diagnosis, probiotics, protein biotechnology, bioleaching, photonic applications and other biotechnology applications. The book will be very useful for Undergraduate and Postgraduate students, research scholars and faculties in biotechnology, microbiology medical sciences and life sciences.

This text follows a body systems approach to microbiology paying attention to real-life connections and covering such topics as the characteristics of microbial metabolism, growth and genetics.

This volume covers the fields of origin, evolution and phylogenesis from prokaryotic to eukaryotic cells. The eminent authors, experts in their fields, review the three kingdoms of life (Archea, Eubacteria and Eukarya) from molecular evolutionary levels to ecological aspects in enigmatic habitats, including general reviews of puzzling pro-and eukaryotic organisms and their domains. We discuss dry habitats, thermophilic (cells in hot springs and undersea thermal vents up to 110°C), psychrophilic (cryophiles) and halophilic (high salt concentrations) niches which among the harshest conditions found on Earth where microbial life is frequently detected. Some chapters deal with the organisms which grow in extreme pH conditions (acidity vs. alkalinity), and under hydrostatic pressure in the deep sea, and microbial growth on petroleum. Audience: Students, lecturers and researchers; scholars in the fields of biology, evolutionary biology and chemistry, and other evolutionary fields, and the intelligent layman.

Highly recommended by CHOICE, Oct 2018 Key features: Couples studies of marine extremophile biology/genomics and extremophile culture for biotechnological applications with the latest advances in bio-prospecting and bio-product development Includes practical experiments that a laboratory can use to replicate extreme habitats for research purposes Presents latest advances in extremophile genomics to give the reader a better understanding of the regulatory mechanisms of extremophiles Offers insights into the production of commercially important extremozymes, carotenoids, bioactive compounds and secondary metabolites of medicinal value Extremophiles are nature’s ultimate survivors, thriving in environments ranging from the frozen Antarctic to abyssal hot hydrothermal vents. Their lifeforms span bacteria to fishes, and are categorized as halophiles from hypersaline environments, acidophiles from acidic waters, psychrophiles from cold habitats, and thermophiles from warm waters. Extremophiles: From Biology to Biotechnology comprehensively covers the basic biology, physiology, habitats, secondary metabolites for bioprospecting, and biotechnology of these extreme survivors. The chapters focus on the novel genetic and biochemical traits that lend these organisms to biotechnological applications. This unique guide serves as a resource for biotechnologists who wish to explore extremophiles for their commercial potential, as well as a valuable reference for teaching undergraduate, graduate and postgraduate students.

The Extremophiles Handbook brings together the rapidly growing and often scattered information on microbial life in the whole range of extreme environments. This book will be a useful reference for finding clues to the origin of life and for exploring the biotechnology potential of these fascinating organisms.

Explores the utility and potential of extremophiles in sustainability and biotechnology Many extremophilic bio-products are already used as life-saving drugs. Until recently, however, the difficulty of working with these microbes has discouraged efforts to develop extremophilic microbes as potential drug reservoirs of the future. Recent technological advances have opened the door to exploring these organisms anew as sources of products that might prove useful in clinical and environmental biotechnology and drug development. Extremophiles features outstanding articles by expert scientists who shed light on broad-ranging areas of progress in the development of smart therapeutics for multiple disease types and products for industrial use. It bridges technological gaps, focusing on critical aspects of extremolytes and the mechanisms regulating their biosynthesis that are relevant to human health and bioenergy, including value-added products of commercial significance as well as other potentially viable products. This groundbreaking guide: Introduces the variety of extremophiles and their extremolytes including extremozymes Provides an overview of the methodologies used to acquire extremophiles Reviews the literature on the diversity of extremophiles Offers tools and criteria for data interpretation of various extremolytes/extremozymes Discusses experimental design problems associated with extremophiles and their therapeutic implications Explores the challenges and possibilities of developing extremolytes for commercial purposes Explains the FDA's regulations on certain microbial bio-products that will be of interest to potential industrialists Extremophiles is an immensely useful resource for graduate students and researchers in biotechnology, clinical biotechnology, microbiology, and applied microbiology.