Light and Video Microscopy

Light and Video Microscopy
Author: Randy O. Wayne
Publsiher: Academic Press
Total Pages: 510
Release: 2019-06-11
ISBN 10: 0128161671
ISBN 13: 9780128161678
Language: EN, FR, DE, ES & NL

Light and Video Microscopy Book Review:

Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope. Provides a fully-revised, updated resource on three-dimensional (3D) structures Contains a new appendices on Diffraction Theory and Advanced Image Processing Provides practical applications, lab exercises and case studies on the mathematics, physics and biology used in microscopy Discusses bright field, dark field, phase-contrast, fluorescence, interference, differential interference and modulation contrast microscopes, oblique illumination and photomicrography

Light and Video Microscopy

Light and Video Microscopy
Author: Randy O. Wayne
Publsiher: Academic Press
Total Pages: 366
Release: 2013-12-16
ISBN 10: 0124115365
ISBN 13: 9780124115361
Language: EN, FR, DE, ES & NL

Light and Video Microscopy Book Review:

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope Clearly develops all ideas from historical and logical foundations Laboratory exercises included to assist the reader with practical applications Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Video Microscopy

Video Microscopy
Author: Shinya Inoue
Publsiher: Springer Science & Business Media
Total Pages: 584
Release: 2013-11-11
ISBN 10: 1475769253
ISBN 13: 9781475769258
Language: EN, FR, DE, ES & NL

Video Microscopy Book Review:

Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.

Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging
Author: Douglas B. Murphy,Michael W. Davidson
Publsiher: John Wiley & Sons
Total Pages: 552
Release: 2012-08-22
ISBN 10: 1118382935
ISBN 13: 9781118382936
Language: EN, FR, DE, ES & NL

Fundamentals of Light Microscopy and Electronic Imaging Book Review:

Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy

Digital Microscopy

Digital Microscopy
Author: Greenfield Sluder,David E. Wolf
Publsiher: Academic Press
Total Pages: 672
Release: 2013-08-07
ISBN 10: 0124078923
ISBN 13: 9780124078925
Language: EN, FR, DE, ES & NL

Digital Microscopy Book Review:

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Video Microscopy

Video Microscopy
Author: Anonim
Publsiher: Academic Press
Total Pages: 334
Release: 1998-04-10
ISBN 10: 9780080859545
ISBN 13: 0080859542
Language: EN, FR, DE, ES & NL

Video Microscopy Book Review:

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Practical laboratory guide for methods and technologies used with video microscopy Comprehensive, easy-to-follow instructions February 1998, c. 334 pp.

Light and Electron Microscopy

Light and Electron Microscopy
Author: Elizabeth M. Slayter,Henry S. Slayter
Publsiher: Cambridge University Press
Total Pages: 312
Release: 1992-10-30
ISBN 10: 9780521339483
ISBN 13: 0521339480
Language: EN, FR, DE, ES & NL

Light and Electron Microscopy Book Review:

Optical and electron microscopes are often used effectively despite little knowledge of the relevant theory or even of how a particular type of microscope functions. Eventually however proper use interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists explains the mechanisms of image formation (contrast and its enhancement) accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are described and there is some discussion of image processing methods. Throughout the text emphasises the underlying similarity of all microscope systems and recognising that biologists may often be uncomfortable with mathematical approaches every effort has been made to present concepts verbally. Where mathematical treatment is indispensible the nature of its contribution is made explicit.

Bioimaging Current Concepts in Light Electron Microscopy

Bioimaging  Current Concepts in Light   Electron Microscopy
Author: Douglas E Chandler,Robert W. Roberson
Publsiher: Jones & Bartlett Publishers
Total Pages: 440
Release: 2009
ISBN 10: 0763738743
ISBN 13: 9780763738747
Language: EN, FR, DE, ES & NL

Bioimaging Current Concepts in Light Electron Microscopy Book Review:

The development of microscopy revolutionized the world of cell and molecular biology as we once knew it and will continue to play an important role in future discoveries. Bioimaging: Current Concepts in Light and Electron Microscopy is the optimal text for any undergraduate or graduate bioimaging course, and will serve as an important reference tool for the research scientist. This unique text covers, in great depth, both light and electron microscopy, as well as other structure and imaging techniques like x-ray crystallography and atomic force microscopy. Written in a user-friendly style and covering a broad range of topics, Bioimaging describes the state-of-the-art technologies that have powered the field to the forefront of cellular and molecular biological research. Important Notice: The digital edition of this book is missing some of the images or content found in the physical edition.

Understanding Light Microscopy

Understanding Light Microscopy
Author: Jeremy Sanderson
Publsiher: Wiley
Total Pages: 848
Release: 2019-05-28
ISBN 10: 0470973757
ISBN 13: 9780470973752
Language: EN, FR, DE, ES & NL

Understanding Light Microscopy Book Review:

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Computer Operation for Microscope Photometry

Computer Operation for Microscope Photometry
Author: H. J. Swatland
Publsiher: CRC Press
Total Pages: 256
Release: 1997-11-25
ISBN 10: 9780849316975
ISBN 13: 0849316979
Language: EN, FR, DE, ES & NL

Computer Operation for Microscope Photometry Book Review:

Suitable for both microscopists seeking computer skills and PC enthusiasts interested in light microscopy, this interdisciplinary text explores the capabilities of the computer-assisted light microscope. Written in clear, simple language, the book explains how computer technology expands the usefulness of the light microscope in spectrophotometry, fluorometry, polarimetry, spatial scanning, and related fields. Beginning with the basic features of light microscopy and personal computer interfacing, the text explains how to make photometric measurements and covers spectrophotometry, stepper motors, and server motors. Polarized light and video image analysis complete this introduction to the field. While software examples are provided to illustrate specific techniques, most operations are described as generalized algorithms that can be adapted to any appropriate high-level language, and used with almost any configuration of the microscope. The book suggests new experiments to inspire further study. Promising new areas of interest, such as the use of fluorescence and polarization, are also included. Computers have radically changed the field of light microscopy in recent decades. Computer Operations for Microscope Photometry helps you master the new techniques.

Correlative Light and Electron MIcroscopy

Correlative Light and Electron MIcroscopy
Author: Anonim
Publsiher: Academic Press
Total Pages: 460
Release: 2012-10-23
ISBN 10: 0123914388
ISBN 13: 9780123914385
Language: EN, FR, DE, ES & NL

Correlative Light and Electron MIcroscopy Book Review:

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods

Optical Microscopy

Optical Microscopy
Author: Brian Herman,John J. Lemasters
Publsiher: Elsevier
Total Pages: 441
Release: 2012-12-02
ISBN 10: 0080571395
ISBN 13: 9780080571393
Language: EN, FR, DE, ES & NL

Optical Microscopy Book Review:

Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances Caged compounds, fluorescence ratio imaging, and CCD video cameras Simultaneous multiple detection and real-time fluorescence microscopy Simultaneous DIC and quantitative LLF video imaging Total internal reflectance, time-resolved, and automated fluorescence microscopy Laser-scanning confocal microscopy Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells

New Techniques of Optical Microscopy and Microspectroscopy

New Techniques of Optical Microscopy and Microspectroscopy
Author: Cherry
Publsiher: CRC Press
Total Pages: 279
Release: 1991
ISBN 10: 9780849371172
ISBN 13: 0849371171
Language: EN, FR, DE, ES & NL

New Techniques of Optical Microscopy and Microspectroscopy Book Review:

The last few years have seen an upsurge of interest in the study of cells by optical microscopy. The advent of new techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutioniz-ing the field. A number of groups have developed new ways of making spectroscopic measurements at the microscopic level, accompanied by the introduction of appropriate sen-sor molecules for cellular assays. The aim of this volume will be to bring together the various advances in order to provide the reader with an up-to-date account of what can now be achieved with modern optical microscopic methods.

Coherent Light Microscopy

Coherent Light Microscopy
Author: Pietro Ferraro,Adam Wax,Zeev Zalevsky
Publsiher: Springer Science & Business Media
Total Pages: 372
Release: 2011-02-09
ISBN 10: 9783642158131
ISBN 13: 3642158137
Language: EN, FR, DE, ES & NL

Coherent Light Microscopy Book Review:

This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Hsiung s Diagnostic Virology

Hsiung s Diagnostic Virology
Author: Gueh Djen Hsiung,Caroline K. Y. Fong,Marie L. Landry
Publsiher: Yale University Press
Total Pages: 382
Release: 1994-01-01
ISBN 10: 9780300058451
ISBN 13: 0300058454
Language: EN, FR, DE, ES & NL

Hsiung s Diagnostic Virology Book Review:

This generously illustrated book, the fourth edition of the highly acclaimed Diagnostic Virology, is a handbook for the recognition and characterization of virus-induced cellular changes seen by light microscopy, as well as virus morphology seen by electron microscopy. The authors cover all aspects of the subject, from specimen collection and laboratory safety to virus isolation and identification and cell culture. This edition includes a new chapter on the human retroviruses, especially relevant since the onset of the AIDS epidemic, and expands the chapters on the arboviruses and hepatitis viruses.

Fundamentals of Fluorescence Microscopy

Fundamentals of Fluorescence Microscopy
Author: Partha Pratim Mondal,Alberto Diaspro
Publsiher: Springer Science & Business Media
Total Pages: 218
Release: 2013-12-12
ISBN 10: 9400775458
ISBN 13: 9789400775459
Language: EN, FR, DE, ES & NL

Fundamentals of Fluorescence Microscopy Book Review:

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Visualization of Receptors

Visualization of Receptors
Author: Gerard Morel
Publsiher: CRC Press
Total Pages: 448
Release: 1997-02-26
ISBN 10: 9780849326448
ISBN 13: 0849326443
Language: EN, FR, DE, ES & NL

Visualization of Receptors Book Review:

A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.

Introduction to Light Microscopy

Introduction to Light Microscopy
Author: Savile Bradbury,Brian Bracegirdle
Publsiher: Bios Scientific Pub Limited
Total Pages: 123
Release: 1998
ISBN 10: 9780387915159
ISBN 13: 038791515X
Language: EN, FR, DE, ES & NL

Introduction to Light Microscopy Book Review:

This book provides detailed and fully illustrated advice on choosing and using the appropriate type of light microscope for a particular application. The low-power stereomicroscope is described, and the many different types of condensers, objectives and eyepieces required for the high-power compound microscope are explained in detail. The book also describes the correct care and use of the microscope in order to achieve the best possible image, and provides a checklist to aid in the diagnosis and correction of problems. Practical step-by-step guidance ensures that the reader always obtains a clear image, Introduction to Light Microscopy is therefore an essential guide for amateur and professional users of the light microscope in all areas of science.

Introduction to Electron Microscopy for Biologists

Introduction to Electron Microscopy for Biologists
Author: Anonim
Publsiher: Academic Press
Total Pages: 560
Release: 2008-10-22
ISBN 10: 008088816X
ISBN 13: 9780080888163
Language: EN, FR, DE, ES & NL

Introduction to Electron Microscopy for Biologists Book Review:

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers). *Basic techniques in transmission and scanning electron microscopy *Detailed chapters on how to use electron microscopy when dealing with specific cellular structures, such as the nucleus, cell membrane, and cytoskeleton *Discussion on electron microscopy of viruses and virus-cell interactions

Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging
Author: Douglas B. Murphy
Publsiher: John Wiley & Sons
Total Pages: 384
Release: 2002-06-18
ISBN 10: 047123429X
ISBN 13: 9780471234296
Language: EN, FR, DE, ES & NL

Fundamentals of Light Microscopy and Electronic Imaging Book Review:

Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope-skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as: * Illuminators, filters, and isolation of specific wavelengths * Phase contrast and differential interference contrast * Properties of polarized light and polarization microscopy * Fluorescence and confocal laser scanning microscopy * Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. An essential resource for both, experienced and novice microscopists.