Genome Engineering via CRISPR Cas9 System

Genome Engineering via CRISPR Cas9 System
Author: Vijai Singh,Pawan K. Dhar
Publsiher: Academic Press
Total Pages: 384
Release: 2020-02-18
ISBN 10: 0128181419
ISBN 13: 9780128181416
Language: EN, FR, DE, ES & NL

Genome Engineering via CRISPR Cas9 System Book Review:

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

Genome Editing and Engineering

Genome Editing and Engineering
Author: Krishnarao Appasani
Publsiher: Cambridge University Press
Total Pages: 586
Release: 2018-07-31
ISBN 10: 1107170370
ISBN 13: 9781107170377
Language: EN, FR, DE, ES & NL

Genome Editing and Engineering Book Review:

A complete guide to endonuclease-based genomic engineering, from basic science to application in disease biology and clinical treatment.

Plant Genome Editing with CRISPR Systems

Plant Genome Editing with CRISPR Systems
Author: Yiping Qi
Publsiher: Humana Press
Total Pages: 367
Release: 2019-02-19
ISBN 10: 9781493989904
ISBN 13: 1493989901
Language: EN, FR, DE, ES & NL

Plant Genome Editing with CRISPR Systems Book Review:

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Human Genome Editing

Human Genome Editing
Author: National Academies of Sciences, Engineering, and Medicine,National Academy of Medicine,National Academy of Sciences,Committee on Human Gene Editing: Scientific, Medical, and Ethical Considerations
Publsiher: National Academies Press
Total Pages: 328
Release: 2017-08-13
ISBN 10: 0309452880
ISBN 13: 9780309452885
Language: EN, FR, DE, ES & NL

Human Genome Editing Book Review:

Genome editing is a powerful new tool for making precise alterations to an organism's genetic material. Recent scientific advances have made genome editing more efficient, precise, and flexible than ever before. These advances have spurred an explosion of interest from around the globe in the possible ways in which genome editing can improve human health. The speed at which these technologies are being developed and applied has led many policymakers and stakeholders to express concern about whether appropriate systems are in place to govern these technologies and how and when the public should be engaged in these decisions. Human Genome Editing considers important questions about the human application of genome editing including: balancing potential benefits with unintended risks, governing the use of genome editing, incorporating societal values into clinical applications and policy decisions, and respecting the inevitable differences across nations and cultures that will shape how and whether to use these new technologies. This report proposes criteria for heritable germline editing, provides conclusions on the crucial need for public education and engagement, and presents 7 general principles for the governance of human genome editing.

Next Generation Plant Breeding

Next Generation Plant Breeding
Author: Yelda Ozden Çiftçi
Publsiher: BoD – Books on Demand
Total Pages: 160
Release: 2018-09-26
ISBN 10: 1789239257
ISBN 13: 9781789239256
Language: EN, FR, DE, ES & NL

Next Generation Plant Breeding Book Review:

Following the green revolution and transgenic crop development, another revolutionary progress has been experienced in plant breeding in the last decade with the application of marker-assisted selection (MAS), next-generation sequencing (NGS), and gene editing techniques together with omic technologies, including genomics, transcriptomics, proteomics, and phenomics. Thus, this book is structured into two sections: "Marker-Assisted Breeding" and "RNA-seq and Gene Editing in Plants," which aim to provide a reference for students, instructors, and scientists on recent innovative advances in plant-breeding programs to cultivate crops for tomorrow.

CRISPR Cas Systems

CRISPR Cas Systems
Author: Rodolphe Barrangou,John van der Oost
Publsiher: Springer Science & Business Media
Total Pages: 302
Release: 2012-12-13
ISBN 10: 364234657X
ISBN 13: 9783642346576
Language: EN, FR, DE, ES & NL

CRISPR Cas Systems Book Review:

CRISPR/Cas is a recently described defense system that protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR/Cas systems has been identified in at least half of the available prokaryotic genomes. On-going structural and functional analyses have resulted in a far greater insight into the functions and possible applications of these systems, although many secrets remain to be discovered. In this book, experts summarize the state of the art in this exciting field.

Chromosomal Mutagenesis

Chromosomal Mutagenesis
Author: Shondra M. Pruett-Miller
Publsiher: Humana Press
Total Pages: 333
Release: 2016-09-22
ISBN 10: 9781493955411
ISBN 13: 1493955411
Language: EN, FR, DE, ES & NL

Chromosomal Mutagenesis Book Review:

This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.

Targeted Genome Editing Using Site Specific Nucleases

Targeted Genome Editing Using Site Specific Nucleases
Author: Takashi Yamamoto
Publsiher: Springer
Total Pages: 205
Release: 2015-01-05
ISBN 10: 4431552278
ISBN 13: 9784431552277
Language: EN, FR, DE, ES & NL

Targeted Genome Editing Using Site Specific Nucleases Book Review:

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Advances in Genome Editing

Advances in Genome Editing
Author: Jennifer A. Doudna
Publsiher: Unknown
Total Pages: 329
Release: 2016
ISBN 10: 9781618962331
ISBN 13: 1618962337
Language: EN, FR, DE, ES & NL

Advances in Genome Editing Book Review:

Gene Editing

Gene Editing
Author: Yuan-Chuan Chen,Shiu-Jau Chen
Publsiher: BoD – Books on Demand
Total Pages: 108
Release: 2019-05-29
ISBN 10: 1789845084
ISBN 13: 9781789845082
Language: EN, FR, DE, ES & NL

Gene Editing Book Review:

Gene-editing technologies (e.g., ZFNs, TALENs, and CRISPRs/Cas9) have been extensively used as tools in basic research. They are further applied in manufacturing agricultural products, food, industrial products, medicinal products, etc. Particularly, the discovery of medicinal products using gene-editing technologies will open a new era for human therapeutics. Though there are still many technical and ethical challenges ahead of us, more and more products based on gene-editing technologies have been approved for marketing. These technologies are promising for multiple applications. Their development and implications should be explored in the broadest context possible. Future research directions should also be highlighted. In this book, the applications, perspectives, and challenges of gene-editing technologies are significantly demonstrated and discussed.

Conditional Mutagenesis An Approach to Disease Models

Conditional Mutagenesis  An Approach to Disease Models
Author: Robert Feil,Daniel Metzger
Publsiher: Springer Science & Business Media
Total Pages: 532
Release: 2007-02-01
ISBN 10: 3540351094
ISBN 13: 9783540351092
Language: EN, FR, DE, ES & NL

Conditional Mutagenesis An Approach to Disease Models Book Review:

In this book, leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse and their application to model human physiology and pathophysiology. The book illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. In addition, it considers the development of new drugs to treat human diseases.

CRISPR Gene Editing

CRISPR Gene Editing
Author: Yonglun Luo
Publsiher: Unknown
Total Pages: 362
Release: 2019
ISBN 10: 9781493991709
ISBN 13: 1493991701
Language: EN, FR, DE, ES & NL

CRISPR Gene Editing Book Review:

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

CRISPR and RNAi Systems

CRISPR and RNAi Systems
Author: Kamel A. Abd-Elsalam,Ki-Taek Lim
Publsiher: Elsevier
Total Pages: 840
Release: 2021-02-27
ISBN 10: 0128219114
ISBN 13: 9780128219119
Language: EN, FR, DE, ES & NL

CRISPR and RNAi Systems Book Review:

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

The Use of CRISPR cas9 ZFNs TALENs in Generating Site Specific Genome Alterations

The Use of CRISPR cas9  ZFNs  TALENs in Generating Site Specific Genome Alterations
Author: Anonim
Publsiher: Academic Press
Total Pages: 570
Release: 2014-11-04
ISBN 10: 0128013346
ISBN 13: 9780128013342
Language: EN, FR, DE, ES & NL

The Use of CRISPR cas9 ZFNs TALENs in Generating Site Specific Genome Alterations Book Review:

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease

Plant Genomes

Plant Genomes
Author: Jean-Nicolas Volff
Publsiher: Karger Medical and Scientific Publishers
Total Pages: 145
Release: 2008-01-01
ISBN 10: 3805584911
ISBN 13: 9783805584913
Language: EN, FR, DE, ES & NL

Plant Genomes Book Review:

Recent major advances in the field of comparative genomics and cytogenomics of plants, particularly associated with the completion of ambitious genome projects, have uncovered astonishing facets of the architecture and evolutionary history of plant genomes. The aim of this book was to review these recent developments as well as their implications in our understanding of the mechanisms which drive plant diversity. New insights into the evolution of gene functions, gene families and genome size are presented, with particular emphasis on the evolutionary impact of polyploidization and transposable elements. Knowledge on the structure and evolution of plant sex chromosomes, centromeres and microRNAs is reviewed and updated. Taken together, the contributions by internationally recognized experts present a panoramic overview of the structural features and evolutionary dynamics of plant genomes.This volume of Genome Dynamics will provide researchers, teachers and students in the fields of biology and agronomy with a valuable source of current knowledge on plant genomes.

Editing Humanity

Editing Humanity
Author: Kevin Davies
Publsiher: Simon and Schuster
Total Pages: 336
Release: 2020-10-06
ISBN 10: 1643133942
ISBN 13: 9781643133942
Language: EN, FR, DE, ES & NL

Editing Humanity Book Review:

One of the world's leading experts on genetics unravels one of the most important breakthroughs in modern science and medicine. IIf our genes are, to a great extent, our destiny, then what would happen if mankind could engineer and alter the very essence of our DNA coding? Millions might be spared the devastating effects of hereditary disease or the challenges of disability, whether it was the pain of sickle-cell anemia to the ravages of Huntington’s disease. But this power to “play God” also raises major ethical questions and poses threats for potential misuse. For decades, these questions have lived exclusively in the realm of science fiction, but as Kevin Davies powerfully reveals in his new book, this is all about to change. Engrossing and page-turning, Editing Humanity takes readers inside the fascinating world of a new gene editing technology called CRISPR, a high-powered genetic toolkit that enables scientists to not only engineer but to edit the DNA of any organism down to the individual building blocks of the genetic code. Davies introduces readers to arguably the most profound scientific breakthrough of our time. He tracks the scientists on the front lines of its research to the patients whose powerful stories bring the narrative movingly to human scale. Though the birth of the “CRISPR babies” in China made international news, there is much more to the story of CRISPR than headlines seemingly ripped from science fiction. In Editing Humanity, Davies sheds light on the implications that this new technology can have on our everyday lives and in the lives of generations to come.

Gene Function

Gene Function
Author: S. Rosenthal,H. Bielka,Ch. Coutelle
Publsiher: Elsevier
Total Pages: 564
Release: 2014-05-09
ISBN 10: 1483188515
ISBN 13: 9781483188515
Language: EN, FR, DE, ES & NL

Gene Function Book Review:

Gene Function, contains the proceedings of the 12th Meeting of the Federation of European Biochemical Societies held in Dresden, Germany in 1978. The meeting provided a forum for discussing progress in the understanding of gene function and covered topics ranging from the functional organization of chromatin to principles of interactions and recognition models. The role of DNA sequence in the recognition of restriction endonucleases and modification enzymes is also examined, along with gene expression, RNA processing and modification, and isolation and synthesis of genes. Comprised of 49 chapters, this volume begins with an overview of what can be learned from the genetic analysis of the lac repressor, followed by a discussion on the topography of the interaction the lac repressor, RNA polymerase, and histones with DNA. The reader is then introduced to complementarity and recognition code between regulatory proteins and DNA; chromatin replication in vitro; and the cytoplasmic "petite" mutation in Saccharomyces cerevisiae. Subsequent chapters explore arc-like and helical arrangements of nucleosome cores; changes in gene expression during cellular differentiation; polyadenylation and processing of pre-messenger RNA; and the molecular biology of bacteriophages T3 and T7. This book will be of interest to geneticists, biochemists, and molecular biologists.

Diversity and Evolution of Butterfly Wing Patterns

Diversity and Evolution of Butterfly Wing Patterns
Author: Toshio Sekimura,H. Frederik Nijhout
Publsiher: Springer
Total Pages: 321
Release: 2017-08-29
ISBN 10: 9811049564
ISBN 13: 9789811049569
Language: EN, FR, DE, ES & NL

Diversity and Evolution of Butterfly Wing Patterns Book Review:

This book facilitates an integrative understanding of the development, genetics and evolution of butterfly wing patterns. To develop a deep and realistic understanding of the diversity and evolution of butterfly wing patterns, it is essential and necessary to approach the problem from various kinds of key research fields such as “evo-devo,” “eco-devo,” ”developmental genetics,” “ecology and adaptation,” “food plants,” and “theoretical modeling.” The past decade-and-a-half has seen a veritable revolution in our understanding of the development, genetics and evolution of butterfly wing patterns. In addition, studies of how environmental and climatic factors affect the expression of color patterns has led to increasingly deeper understanding of the pervasiveness and underlying mechanisms of phenotypic plasticity. In recognition of the great progress in research on the biology, an international meeting titled “Integrative Approach to Understanding the Diversity of Butterfly Wing Patterns (IABP-2016)” was held at Chubu University, Japan in August 2016. This book consists of selected contributions from the meeting. Authors include main active researchers of new findings of corresponding genes as well as world leaders in both experimental and theoretical approaches to wing color patterns. The book provides excellent case studies for graduate and undergraduate classes in evolution, genetics/genomics, developmental biology, ecology, biochemistry, and also theoretical biology, opening the door to a new era in the integrative approach to the analysis of biological problems. This book is open access under a CC BY 4.0 license.

Chromosome and Genomic Engineering in Plants

Chromosome and Genomic Engineering in Plants
Author: Minoru Murata
Publsiher: Humana Press
Total Pages: 218
Release: 2016-08-25
ISBN 10: 9781493949298
ISBN 13: 1493949292
Language: EN, FR, DE, ES & NL

Chromosome and Genomic Engineering in Plants Book Review:

This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.

CRISPR Cas

CRISPR Cas
Author: University Jennifer Doudna,Prashant Mali
Publsiher: Unknown
Total Pages: 192
Release: 2016-03-23
ISBN 10: 9781621821304
ISBN 13: 1621821307
Language: EN, FR, DE, ES & NL

CRISPR Cas Book Review:

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.