FRET and FLIM Techniques

FRET and FLIM Techniques
Author: Theodorus W. J. Gadella
Publsiher: Elsevier
Total Pages: 560
Release: 2011-07-29
ISBN 10: 9780080915128
ISBN 13: 0080915124
Language: EN, FR, DE, ES & NL

FRET and FLIM Techniques Book Review:

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques
Author: Theodorus W. J. Gadella
Publsiher: Elsevier Science
Total Pages: 560
Release: 2009-01-05
ISBN 10: 9780080549583
ISBN 13: 0080549586
Language: EN, FR, DE, ES & NL

FRET and FLIM Techniques Book Review:

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Methods in Cellular Imaging

Methods in Cellular Imaging
Author: Ammasi Periasamy
Publsiher: Springer
Total Pages: 448
Release: 2013-05-27
ISBN 10: 1461475139
ISBN 13: 9781461475132
Language: EN, FR, DE, ES & NL

Methods in Cellular Imaging Book Review:

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Multiphoton Microscopy and Fluorescence Lifetime Imaging
Author: Karsten König
Publsiher: Walter de Gruyter GmbH & Co KG
Total Pages: 450
Release: 2018-01-22
ISBN 10: 3110429985
ISBN 13: 9783110429985
Language: EN, FR, DE, ES & NL

Multiphoton Microscopy and Fluorescence Lifetime Imaging Book Review:

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Microscopy Techniques

Microscopy Techniques
Author: Jens Rietdorf
Publsiher: Springer Science & Business Media
Total Pages: 319
Release: 2005-06-23
ISBN 10: 9783540236986
ISBN 13: 3540236988
Language: EN, FR, DE, ES & NL

Microscopy Techniques Book Review:

With contributions by numerous experts

Molecular Imaging

Molecular Imaging
Author: Ammasi Periasamy,Richard Day
Publsiher: Elsevier
Total Pages: 312
Release: 2011-04-28
ISBN 10: 9780080536873
ISBN 13: 0080536875
Language: EN, FR, DE, ES & NL

Molecular Imaging Book Review:

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Advanced Time Correlated Single Photon Counting Techniques

Advanced Time Correlated Single Photon Counting Techniques
Author: Wolfgang Becker
Publsiher: Springer Science & Business Media
Total Pages: 401
Release: 2005-12-20
ISBN 10: 9783540288824
ISBN 13: 3540288821
Language: EN, FR, DE, ES & NL

Advanced Time Correlated Single Photon Counting Techniques Book Review:

In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.

Optical Imaging Techniques in Cell Biology

Optical Imaging Techniques in Cell Biology
Author: Guy Cox
Publsiher: CRC Press
Total Pages: 288
Release: 2006-11-06
ISBN 10: 1420005618
ISBN 13: 9781420005615
Language: EN, FR, DE, ES & NL

Optical Imaging Techniques in Cell Biology Book Review:

Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E

FLIM Microscopy in Biology and Medicine

FLIM Microscopy in Biology and Medicine
Author: Ammasi Periasamy,Robert M. Clegg
Publsiher: CRC Press
Total Pages: 472
Release: 2009-07-06
ISBN 10: 9781420078916
ISBN 13: 1420078917
Language: EN, FR, DE, ES & NL

FLIM Microscopy in Biology and Medicine Book Review:

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy’s (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular surroundings of biological molecules. Most of this improvement and discovery have occurred during the past decade, and, to date, information that would benefit a broad range of researchers remains scattered in the literature. Edited by two of the top pioneers in the field, FLIM Microscopy in Biology and Medicine presents the fundamentals of FLIM along with a number of advanced considerations so that a wider audience can appreciate recent and potential improvements that make it such a valuable tool. New Opportunities for Biomedical Researchers... New Challenges for Microscopy Researchers Discussion sections in all the chapters clearly show the challenges for implementing FLIM for various applications. Certain chapters discuss limits on the number of photons required for highly accurate lifetime determinations, as well as the accuracy with which multiple, closely associated lifetime components can reliably be determined. Such considerations are important for the user when he or she is selecting the most advantageous method of FLIM to use for a particular application. While this book provides an introduction for those new to FLIM, it gathers a wealth of material to enhance the work of experts involved in pioneering technological improvements, as well as those research opportunities in this unique and promising area of microscopy.

Time correlated single photon counting

Time correlated single photon counting
Author: Desmond O'Connor
Publsiher: Academic Press
Total Pages: 298
Release: 2012-12-02
ISBN 10: 0323141447
ISBN 13: 9780323141444
Language: EN, FR, DE, ES & NL

Time correlated single photon counting Book Review:

Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.

Integrins and Ion Channels

Integrins and Ion Channels
Author: Andrea Becchetti,Annarosa Arcangeli
Publsiher: Anonim
Total Pages: 127
Release: 2010
ISBN 10: 9781441960733
ISBN 13: 1441960732
Language: EN, FR, DE, ES & NL

Integrins and Ion Channels Book Review:

Interdisciplinarity is more often invoked than practised. This is hardly surprising, considering the daunting vastness of modern biology. To reach a satisfactory understanding of a complex biological system, a wide spectrum of conceptual and experimental tools must be applied at different levels, from the molecular to the cellular, tissue and organismic. We believe the multifaceted regulatory interplay between integrin receptors and ion channels offers a rich and challenging field for researchers seeking broad biological perspectives. By mediating cell adhesion to the extracellular matrix, integrins regulate many developmental processes in the widest sense (from cell choice between differentiation and proliferation, to tissue remodeling and organogenesis). Rapidly growing evidence shows that frequent communication takes place between cell adhesion receptors and channel proteins. This may occur through formation of multiprotein membrane complexes that regulate ion fluxes as well as a variety of intracellular signaling pathways. In other cases, cross talk is more indirect and mediated by cellular messengers such as G proteins. These interactions are reciprocal, in that ion channel stimulation often controls integrin activation or expression. From a functional standpoint, studying the interplay between integrin receptors and ion channels clarifies how the extracellular matrix regulates processes as disparate as muscle excitability, synaptic plasticity and lymphocyte activation, just to mention a few. The derangement of these processes has many implications for pathogenesis processes, in particular for tumor invasiveness and some cardiovascular and neurologic diseases. This book provides a general introduction to the problems and methods of this blossoming field.

Plant Hormones

Plant Hormones
Author: Jürgen Kleine-Vehn,Michael Sauer
Publsiher: Humana Press
Total Pages: 280
Release: 2016-09-13
ISBN 10: 9781493964673
ISBN 13: 1493964674
Language: EN, FR, DE, ES & NL

Plant Hormones Book Review:

This volume aims to present a representative cross-section of modern experimental approaches relevant to Plant Hormone Biology, ranging from relatively simple physiological to highly sophisticated methods. Chapters describe physiological, developmental, microscopy-based techniques, measure hormone contents, and heterologous systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Hormones:Methods and Protocols, Third Edition aims to provide researchers with useful methods to advance their research.

Biomedical Optical Imaging Technologies

Biomedical Optical Imaging Technologies
Author: Rongguang Liang
Publsiher: Springer Science & Business Media
Total Pages: 382
Release: 2012-09-21
ISBN 10: 364228390X
ISBN 13: 9783642283901
Language: EN, FR, DE, ES & NL

Biomedical Optical Imaging Technologies Book Review:

This book provides an introduction to design of biomedical optical imaging technologies and their applications. The main topics include: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic systems. Each chapter is written by the world leaders of the respective fields, and will cover: principles and limitations of optical imaging technology, system design and practical implementation for one or two specific applications, including design guidelines, system configuration, optical design, component requirements and selection, system optimization and design examples, recent advances and applications in biomedical researches and clinical imaging. This book serves as a reference for students and researchers in optics and biomedical engineering.

Fluorescent Methods to Study Biological Membranes

Fluorescent Methods to Study Biological Membranes
Author: Yves Mely,Guy Duportail
Publsiher: Springer Science & Business Media
Total Pages: 486
Release: 2012-10-10
ISBN 10: 3642331270
ISBN 13: 9783642331275
Language: EN, FR, DE, ES & NL

Fluorescent Methods to Study Biological Membranes Book Review:

Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Advanced Imaging Techniques in Clinical Pathology

Advanced Imaging Techniques in Clinical Pathology
Author: Francesco M. Sacerdoti,Antonio Giordano,Carlo Cavaliere
Publsiher: Humana Press
Total Pages: 165
Release: 2016-08-23
ISBN 10: 1493934694
ISBN 13: 9781493934690
Language: EN, FR, DE, ES & NL

Advanced Imaging Techniques in Clinical Pathology Book Review:

This text provides a comprehensive, state-of-the-art review of the application of image analysis focusing on the techniques which can be used in every biology and medical laboratory to automate procedures of cell analysis and to create statistics very useful for a comprehension of cell growth dynamics and the effects of drugs on them. This textbook will serve as a very useful resource for physicians and researchers dealing with, and interested in, cell analysis. It will provide a concise yet comprehensive summary of the current status of the field that will help guide patient management and stimulate investigative efforts. All chapters are written by experts in their fields and include the most up-to-date scientific and clinical information. Advanced Imaging Techniques in Clinical Pathology will be of great value to clinical pathologists, biologists, biology researchers, and those working in the clinical and biological laboratory arena.​

Bio nanoimaging

Bio nanoimaging
Author: Vladimir Uversky,Yuri Lyubchenko
Publsiher: Academic Press
Total Pages: 552
Release: 2013-11-05
ISBN 10: 0123978211
ISBN 13: 9780123978219
Language: EN, FR, DE, ES & NL

Bio nanoimaging Book Review:

Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin oligomers - Polymorphisms of misfolding and aggregation processes, including multiple pathways of lysozyme aggregation, misfolded intermediate of a PDZ domain, and micelle formation by human islet amyloid polypeptide Protein misfolding and aggregation is a fast-growing frontier in molecular medicine and protein chemistry. Related disorders include cataracts, arthritis, cystic fibrosis, late-onset diabetes mellitus, and numerous neurodegenerative diseases like Alzheimer's and Parkinson's. Nanoimaging technology has proved crucial in understanding protein-misfolding pathologies and in potential drug design aimed at the inhibition or reversal of protein aggregation. Using these technologies, researchers can monitor the aggregation process, visualize protein aggregates and analyze their properties. Provides practical examples of nanoimaging research from leading molecular biology, cell biology, protein chemistry, biotechnology, genetics, and pharmaceutical labs Includes over 200 color images to illustrate the power of various nanoimaging technologies Focuses on nanoimaging techniques applied to protein misfolding and aggregation in molecular medicine

Molecular Fluorescence

Molecular Fluorescence
Author: Bernard Valeur,Mário Nuno Berberan-Santos
Publsiher: John Wiley & Sons
Total Pages: 569
Release: 2012-05-29
ISBN 10: 3527328378
ISBN 13: 9783527328376
Language: EN, FR, DE, ES & NL

Molecular Fluorescence Book Review:

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

Principles of Fluorescence Spectroscopy

Principles of Fluorescence Spectroscopy
Author: Joseph R. Lakowicz
Publsiher: Springer Science & Business Media
Total Pages: 698
Release: 2013-04-17
ISBN 10: 1475730616
ISBN 13: 9781475730616
Language: EN, FR, DE, ES & NL

Principles of Fluorescence Spectroscopy Book Review:

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Advanced Optical Methods for Brain Imaging

Advanced Optical Methods for Brain Imaging
Author: Fu-Jen Kao,Gerd Keiser,Ankur Gogoi
Publsiher: Springer
Total Pages: 334
Release: 2018-05-14
ISBN 10: 9811090203
ISBN 13: 9789811090202
Language: EN, FR, DE, ES & NL

Advanced Optical Methods for Brain Imaging Book Review:

This book highlights the rapidly developing field of advanced optical methods for structural and functional brain imaging. As is known, the brain is the most poorly understood organ of a living body. It is indeed the most complex structure in the known universe and, thus, mapping of the brain has become one of the most exciting frontlines of contemporary research. Starting from the fundamentals of the brain, neurons and synapses, this book presents a streamlined and focused coverage of the core principles, theoretical and experimental approaches, and state-of-the-art applications of most of the currently used imaging methods in brain research. It presents contributions from international leaders on different photonics-based brain imaging modalities and techniques. Included are comprehensive descriptions of many of the technology driven spectacular advances made over the past few years that have allowed novel insights of the structural and functional details of neurons. The book is targeted at researchers, engineers and scientists who are working in the field of brain imaging, neuroscience and connectomics. Although this book is not intended to serve as a textbook, it will appeal to undergraduate students engaged in the specialization of brain imaging.

Fluorescence Microscopy

Fluorescence Microscopy
Author: Ulrich Kubitscheck
Publsiher: John Wiley & Sons
Total Pages: 504
Release: 2017-05-08
ISBN 10: 3527338373
ISBN 13: 9783527338375
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy Book Review:

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.