FRET and FLIM Techniques

FRET and FLIM Techniques
Author: Theodorus W. J. Gadella
Publsiher: Elsevier
Total Pages: 560
Release: 2011-07-29
ISBN 10: 9780080915128
ISBN 13: 0080915124
Language: EN, FR, DE, ES & NL

FRET and FLIM Techniques Book Review:

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

FRET and FLIM Techniques

FRET and FLIM Techniques
Author: Theodorus W. J. Gadella
Publsiher: Elsevier Science
Total Pages: 560
Release: 2009-01-05
ISBN 10: 9780080549583
ISBN 13: 0080549586
Language: EN, FR, DE, ES & NL

FRET and FLIM Techniques Book Review:

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Laboratory techniques in biochemistry and molecular biology

Laboratory techniques in biochemistry and molecular biology
Author: Theodorus Wilhelmus Johannes Gadella
Publsiher: Unknown
Total Pages: 534
Release: 2009
ISBN 10: 1928374650XXX
ISBN 13: OCLC:928804837
Language: EN, FR, DE, ES & NL

Laboratory techniques in biochemistry and molecular biology Book Review:

Molecular Imaging

Molecular Imaging
Author: Ammasi Periasamy,Richard Day
Publsiher: Elsevier
Total Pages: 312
Release: 2011-04-28
ISBN 10: 9780080536873
ISBN 13: 0080536875
Language: EN, FR, DE, ES & NL

Molecular Imaging Book Review:

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Multiphoton Microscopy and Fluorescence Lifetime Imaging
Author: Karsten König
Publsiher: Walter de Gruyter GmbH & Co KG
Total Pages: 450
Release: 2018-01-22
ISBN 10: 311043007X
ISBN 13: 9783110430073
Language: EN, FR, DE, ES & NL

Multiphoton Microscopy and Fluorescence Lifetime Imaging Book Review:

This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Methods in Cellular Imaging

Methods in Cellular Imaging
Author: Ammasi Periasamy
Publsiher: Springer
Total Pages: 448
Release: 2013-05-27
ISBN 10: 1461475139
ISBN 13: 9781461475132
Language: EN, FR, DE, ES & NL

Methods in Cellular Imaging Book Review:

Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.

FRET and FLIM Techniques

FRET and FLIM Techniques
Author: Theodorus W. J. Gadella
Publsiher: Elsevier Science
Total Pages: 560
Release: 2009-01-05
ISBN 10: 9780080549583
ISBN 13: 0080549586
Language: EN, FR, DE, ES & NL

FRET and FLIM Techniques Book Review:

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Methods in Cell Biology

Methods in Cell Biology
Author: Anonim
Publsiher: Academic Press
Total Pages: 194
Release: 2020-05-16
ISBN 10: 012820009X
ISBN 13: 9780128200094
Language: EN, FR, DE, ES & NL

Methods in Cell Biology Book Review:

Methods in Cell Biology, Volume 158, the latest release in this series, highlights new advances in the field, with this release covering How to orient cells in micro-cavities for high resolution imaging of cytokinesis and lumen formation, A body-on-a-chip (BOC) system for studying gut-liver interaction, Manipulating cultured mammalian cells for mitosis research, Live-cell FLIM-FRET using a commercially available system, A comparative analysis of methods to measure kinetochore-microtubule attachment stability, A workflow for visualizing human cancer biopsies using large-format electron microscopy, Isolation of stage-specific germ cells using facs in drosophila germarium, Computational analysis of filament polymerization dynamics in cytoskeletal networks, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Updated release includes the latest information in this area of study

Understanding Light Microscopy

Understanding Light Microscopy
Author: Jeremy Sanderson
Publsiher: John Wiley & Sons
Total Pages: 848
Release: 2019-03-04
ISBN 10: 1118696743
ISBN 13: 9781118696743
Language: EN, FR, DE, ES & NL

Understanding Light Microscopy Book Review:

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Time correlated single photon counting

Time correlated single photon counting
Author: Desmond O'Connor
Publsiher: Academic Press
Total Pages: 298
Release: 2012-12-02
ISBN 10: 0323141447
ISBN 13: 9780323141444
Language: EN, FR, DE, ES & NL

Time correlated single photon counting Book Review:

Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.

Integrins and Ion Channels

Integrins and Ion Channels
Author: Andrea Becchetti,Annarosa Arcangeli
Publsiher: Springer Science & Business Media
Total Pages: 124
Release: 2010-12-28
ISBN 10: 144196066X
ISBN 13: 9781441960665
Language: EN, FR, DE, ES & NL

Integrins and Ion Channels Book Review:

Interdisciplinarity is more often invoked than practised. This is hardly surprising, considering the daunting vastness of modern biology. To reach a satisfactory understanding of a complex biological system, a wide spectrum of conceptual and experimental tools must be applied at different levels, from the molecular to the cellular, tissue and organismic. We believe the multifaceted regulatory interplay between integrin receptors and ion channels offers a rich and challenging field for researchers seeking broad biological perspectives. By mediating cell adhesion to the extracellular matrix, integrins regulate many developmental processes in the widest sense (from cell choice between differentiation and proliferation, to tissue remodeling and organogenesis). Rapidly growing evidence shows that frequent communication takes place between cell adhesion receptors and channel proteins. This may occur through formation of multiprotein membrane complexes that regulate ion fluxes as well as a variety of intracellular signaling pathways. In other cases, cross talk is more indirect and mediated by cellular messengers such as G proteins. These interactions are reciprocal, in that ion channel stimulation often controls integrin activation or expression. From a functional standpoint, studying the interplay between integrin receptors and ion channels clarifies how the extracellular matrix regulates processes as disparate as muscle excitability, synaptic plasticity and lymphocyte activation, just to mention a few. The derangement of these processes has many implications for pathogenesis processes, in particular for tumor invasiveness and some cardiovascular and neurologic diseases. This book provides a general introduction to the problems and methods of this blossoming field.

Microscopy Techniques

Microscopy Techniques
Author: Jens Rietdorf
Publsiher: Springer Science & Business Media
Total Pages: 319
Release: 2005-06-23
ISBN 10: 9783540236986
ISBN 13: 3540236988
Language: EN, FR, DE, ES & NL

Microscopy Techniques Book Review:

With contributions by numerous experts

Advanced Time Correlated Single Photon Counting Techniques

Advanced Time Correlated Single Photon Counting Techniques
Author: Wolfgang Becker
Publsiher: Springer Science & Business Media
Total Pages: 401
Release: 2005-12-20
ISBN 10: 3540288821
ISBN 13: 9783540288824
Language: EN, FR, DE, ES & NL

Advanced Time Correlated Single Photon Counting Techniques Book Review:

In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.

Fluorescence Microscopy

Fluorescence Microscopy
Author: Ulrich Kubitscheck
Publsiher: John Wiley & Sons
Total Pages: 496
Release: 2017-03-27
ISBN 10: 3527687726
ISBN 13: 9783527687725
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy Book Review:

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

FLIM Microscopy in Biology and Medicine

FLIM Microscopy in Biology and Medicine
Author: Ammasi Periasamy,Robert M. Clegg
Publsiher: CRC Press
Total Pages: 472
Release: 2009-07-06
ISBN 10: 9781420078916
ISBN 13: 1420078917
Language: EN, FR, DE, ES & NL

FLIM Microscopy in Biology and Medicine Book Review:

Detecting Signals at the Single Molecule Level: Pioneering Achievements in Microscopy Recent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy’s (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional benefit of independence from fluorophore concentration and excitation intensity. Moreover, its unique sensitivity makes it an excellent reporter of conformational changes and of variations in the molecular surroundings of biological molecules. Most of this improvement and discovery have occurred during the past decade, and, to date, information that would benefit a broad range of researchers remains scattered in the literature. Edited by two of the top pioneers in the field, FLIM Microscopy in Biology and Medicine presents the fundamentals of FLIM along with a number of advanced considerations so that a wider audience can appreciate recent and potential improvements that make it such a valuable tool. New Opportunities for Biomedical Researchers... New Challenges for Microscopy Researchers Discussion sections in all the chapters clearly show the challenges for implementing FLIM for various applications. Certain chapters discuss limits on the number of photons required for highly accurate lifetime determinations, as well as the accuracy with which multiple, closely associated lifetime components can reliably be determined. Such considerations are important for the user when he or she is selecting the most advantageous method of FLIM to use for a particular application. While this book provides an introduction for those new to FLIM, it gathers a wealth of material to enhance the work of experts involved in pioneering technological improvements, as well as those research opportunities in this unique and promising area of microscopy.

Advanced Time Correlated Single Photon Counting Applications

Advanced Time Correlated Single Photon Counting Applications
Author: Wolfgang Becker
Publsiher: Springer
Total Pages: 624
Release: 2015-04-13
ISBN 10: 3319149296
ISBN 13: 9783319149295
Language: EN, FR, DE, ES & NL

Advanced Time Correlated Single Photon Counting Applications Book Review:

This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

Biomedical Optical Imaging Technologies

Biomedical Optical Imaging Technologies
Author: Rongguang Liang
Publsiher: Springer Science & Business Media
Total Pages: 382
Release: 2012-09-21
ISBN 10: 364228390X
ISBN 13: 9783642283901
Language: EN, FR, DE, ES & NL

Biomedical Optical Imaging Technologies Book Review:

This book provides an introduction to design of biomedical optical imaging technologies and their applications. The main topics include: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic systems. Each chapter is written by the world leaders of the respective fields, and will cover: principles and limitations of optical imaging technology, system design and practical implementation for one or two specific applications, including design guidelines, system configuration, optical design, component requirements and selection, system optimization and design examples, recent advances and applications in biomedical researches and clinical imaging. This book serves as a reference for students and researchers in optics and biomedical engineering.

Advanced Imaging Techniques in Clinical Pathology

Advanced Imaging Techniques in Clinical Pathology
Author: Francesco M. Sacerdoti,Antonio Giordano,Carlo Cavaliere
Publsiher: Humana Press
Total Pages: 165
Release: 2016-08-23
ISBN 10: 1493934694
ISBN 13: 9781493934690
Language: EN, FR, DE, ES & NL

Advanced Imaging Techniques in Clinical Pathology Book Review:

This text provides a comprehensive, state-of-the-art review of the application of image analysis focusing on the techniques which can be used in every biology and medical laboratory to automate procedures of cell analysis and to create statistics very useful for a comprehension of cell growth dynamics and the effects of drugs on them. This textbook will serve as a very useful resource for physicians and researchers dealing with, and interested in, cell analysis. It will provide a concise yet comprehensive summary of the current status of the field that will help guide patient management and stimulate investigative efforts. All chapters are written by experts in their fields and include the most up-to-date scientific and clinical information. Advanced Imaging Techniques in Clinical Pathology will be of great value to clinical pathologists, biologists, biology researchers, and those working in the clinical and biological laboratory arena.​

Advanced Photon Counting

Advanced Photon Counting
Author: Peter Kapusta,Michael Wahl,Rainer Erdmann
Publsiher: Springer
Total Pages: 370
Release: 2015-04-23
ISBN 10: 3319156365
ISBN 13: 9783319156361
Language: EN, FR, DE, ES & NL

Advanced Photon Counting Book Review:

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy
Author: Alberto Diaspro
Publsiher: CRC Press
Total Pages: 448
Release: 2010-04-26
ISBN 10: 1420078895
ISBN 13: 9781420078893
Language: EN, FR, DE, ES & NL

Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book Review:

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.