Fluorescence Microscopy

Fluorescence Microscopy
Author: Ulrich Kubitscheck
Publsiher: John Wiley & Sons
Total Pages: 504
Release: 2017-05-08
ISBN 10: 3527338373
ISBN 13: 9783527338375
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy Book Review:

While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.

Fluorescence Microscopy

Fluorescence Microscopy
Author: Anda Cornea,P. Michael Conn
Publsiher: Elsevier
Total Pages: 260
Release: 2014-02-24
ISBN 10: 0124167136
ISBN 13: 9780124167131
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy Book Review:

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Introduction to Confocal Fluorescence Microscopy

Introduction to Confocal Fluorescence Microscopy
Author: Michiel Muller
Publsiher: SPIE Press
Total Pages: 119
Release: 2006
ISBN 10: 9780819460431
ISBN 13: 0819460435
Language: EN, FR, DE, ES & NL

Introduction to Confocal Fluorescence Microscopy Book Review:

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Fluorescence Microscopy

Fluorescence Microscopy
Author: Brian Herman
Publsiher: Garland Pub
Total Pages: 170
Release: 1998
ISBN 10: 9781872748849
ISBN 13: 1872748848
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy Book Review:

This volume is a comprehensive revision which combines the theoretical and practical aspects of fluorescence microscopy.

Fluorescence Microscopy

Fluorescence Microscopy
Author: F. W. D. Rost
Publsiher: Cambridge University Press
Total Pages: 473
Release: 1992
ISBN 10: 9780521410885
ISBN 13: 0521410886
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy Book Review:

Fluorescence microscopy is used for studying the distribution of substances which are present in very small amounts, for example in living cells. This magnificent new work provides comprehensive cover of all aspects of fluorescence microscopy - including instrumentation, applications, and the history of the technique. The first volume deals with instrumentation and techniques for fluorescence microscopy, and includes a chapter on quantification and scanning. The second volume deals with the applications of fluorescence microscopy in many fields. It includes information on autofluorescence, and an invaluable appendix provides an alphabetical list of fluorochromes, giving information concerning chemical structure, fluorescence properties, applications and suitable filter combinations.

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy
Author: Alberto Diaspro
Publsiher: CRC Press
Total Pages: 448
Release: 2010-04-26
ISBN 10: 1420078895
ISBN 13: 9781420078893
Language: EN, FR, DE, ES & NL

Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book Review:

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

Fundamentals of Fluorescence Microscopy

Fundamentals of Fluorescence Microscopy
Author: Partha Pratim Mondal,Alberto Diaspro
Publsiher: Springer Science & Business Media
Total Pages: 218
Release: 2013-12-12
ISBN 10: 9400775458
ISBN 13: 9789400775459
Language: EN, FR, DE, ES & NL

Fundamentals of Fluorescence Microscopy Book Review:

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Fluorescence Microscopy in Life Sciences

Fluorescence Microscopy in Life Sciences
Author: Juan Carlos Stockert ,Alfonso Blazquez-Castro
Publsiher: Bentham Science Publishers
Total Pages: 751
Release: 2017-12-15
ISBN 10: 1681085186
ISBN 13: 9781681085180
Language: EN, FR, DE, ES & NL

Fluorescence Microscopy in Life Sciences Book Review:

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.

In Vivo Fluorescence Microscopy of Microcirculation in the Renal Cortex of Mice

In Vivo Fluorescence Microscopy of Microcirculation in the Renal Cortex of Mice
Author: Barry Högström
Publsiher: Unknown
Total Pages: 90
Release: 1993
ISBN 10: 9789171748041
ISBN 13: 9171748040
Language: EN, FR, DE, ES & NL

In Vivo Fluorescence Microscopy of Microcirculation in the Renal Cortex of Mice Book Review:

Far Field Optical Nanoscopy

Far Field Optical Nanoscopy
Author: Philip Tinnefeld,Christian Eggeling,Stefan W. Hell
Publsiher: Springer
Total Pages: 335
Release: 2015-02-07
ISBN 10: 3662455471
ISBN 13: 9783662455470
Language: EN, FR, DE, ES & NL

Far Field Optical Nanoscopy Book Review:

This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Fundamentals of Fluorescence Imaging

Fundamentals of Fluorescence Imaging
Author: Guy Cox
Publsiher: CRC Press
Total Pages: 458
Release: 2019-04-23
ISBN 10: 1351129384
ISBN 13: 9781351129381
Language: EN, FR, DE, ES & NL

Fundamentals of Fluorescence Imaging Book Review:

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Optical Fluorescence Microscopy

Optical Fluorescence Microscopy
Author: Alberto Diaspro
Publsiher: Springer Science & Business Media
Total Pages: 244
Release: 2010-11-25
ISBN 10: 9783642151750
ISBN 13: 3642151752
Language: EN, FR, DE, ES & NL

Optical Fluorescence Microscopy Book Review:

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Nanoscopy and Multidimensional Optical Fluorescence Microscopy
Author: Alberto Diaspro
Publsiher: CRC Press
Total Pages: 448
Release: 2010-04-26
ISBN 10: 1420078895
ISBN 13: 9781420078893
Language: EN, FR, DE, ES & NL

Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book Review:

"Alberto Diaspro has been choreographing light’s dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology’s secrets." — From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimensional Optical Fluorescence Microscopy demonstrates that the boundaries between sciences do blur at the bottom, especially those that might separate the optical work of physicists and the cellular work of microbiologists. In 18 chapters written by pioneering researchers, this work offers the first comprehensive and current documentation of the cutting-edge research being accomplished in a wide range of photonic devices with revolutionary application. The highlight of the book is its coverage of optical nanoscopy and super-resolution microscopy. The rapid advances in this area over the past few years offer researchers in both photonics and molecular biologya wealth of accomplishment upon which they can build. Offering a complete treatment of this emerging field, this volume: Describes how scientists have exploited the properties of light and its fluorophore partners to overcome the resolution limit of conventional light microscopy Delves into recent ways to minimize the photobleaching that has long hampered many methods including those that have the potential to capture previously unobtainable information on the movements of single molecules Discusses the principles, benefits, and implementation of fluorescence correlation spectroscopy and related methods, which simplifies analysis by limiting light to stationary focal points in a sample Considers the most basic as well as emerging methods for improving three-dimensional optical sectioning microscopy Reviews the basics of FRET (fluorescence resonance energy transfer) and considers its new use for investigating protein complexes The text also introduces those emerging nonfluorescence microscopy methods that can actually exert mechanical forces to trap and move a variety of objects ranging from beads to living cells and cellular organelles. Combining this technique with fluorescence microscopy provides an unparalleled ability to manipulate and visualize biological samples. In the half-century since Richard Feynman challenged scientists to come up with the tools to investigate and manipulate our world at the nanoscale, we have succeeded in placing tools in the hands of biophysicists that are leading to major breakthroughs in our understanding of life and our ability to diagnose, treat, and prevent many challenges to human health. This book reflects what has been accomplished to date while pointing the way to what still needs to be done.

The Use of Fluorescence Microscopy in Studies of Self incompatibility in Cabbage

The Use of Fluorescence Microscopy in Studies of Self incompatibility in Cabbage
Author: John Carl Webster
Publsiher: Unknown
Total Pages: 276
Release: 1973
ISBN 10:
ISBN 13: CORNELL:31924000753107
Language: EN, FR, DE, ES & NL

The Use of Fluorescence Microscopy in Studies of Self incompatibility in Cabbage Book Review:

Introduction to Fluorescence Microscopy

Introduction to Fluorescence Microscopy
Author: J. S. Ploem,H. J. Tanke
Publsiher: Garland Science
Total Pages: 56
Release: 1987
ISBN 10:
ISBN 13: UOM:39015012610625
Language: EN, FR, DE, ES & NL

Introduction to Fluorescence Microscopy Book Review:

Covers three main areas.The phenomenon of fluorescence, the main applications, and the most common problems.

Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy
Author: James Pawley
Publsiher: Springer Science & Business Media
Total Pages: 632
Release: 2013-04-17
ISBN 10: 1475753489
ISBN 13: 9781475753486
Language: EN, FR, DE, ES & NL

Handbook of Biological Confocal Microscopy Book Review:

This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Ultra high Resolution Fluorescence Microscopy and Its Application in Biology

Ultra high Resolution Fluorescence Microscopy and Its Application in Biology
Author: Lin Shao
Publsiher: Unknown
Total Pages: 76
Release: 2005
ISBN 10:
ISBN 13: UCAL:X85644
Language: EN, FR, DE, ES & NL

Ultra high Resolution Fluorescence Microscopy and Its Application in Biology Book Review:

Several new resolution enhancement schemes for fluorescence microscopy are experimentally implemented and tested using beads and biological samples. The first scheme, 3D structured illumination microscopy (SIM), makes use of the simple fact that fluorescence emission is the multiplication of the illumination and the sample's dye structure. If the illumination is structured such as a sinusoidal pattern, the recorded image would contain high-frequency components encoded in terms of low frequencies. In so doing, normally undetectable high-resolution information is given a chance to appear in the observable region, and is thus made effectively detectable. We are able to double the resolution both laterally and axially of the conventional microscope with 3D SIM. The second scheme, called I5S, also uses structured illumination, but with a Sagnac-like interferometer setup consisting of two opposing objective lenses used for illumination and fluorescence detection. Interference in both illumination, which gives rise to more complicated structured illumination pattern than 3D SIM, and detection can be achieved. The combined interferometric effect is seven-fold enhancement in axial resolution as well as doubled lateral resolution. Finally, we introduce a more complicated I5S scheme in which both emission wavefronts, instead of just one of them, are recorded by two CCD cameras simultaneously. The complementary phase relationship between the two wavefronts can be utilized in estimating and then correcting the phase error often difficult to avoid in I5S experiments.

Light and Video Microscopy

Light and Video Microscopy
Author: Randy O. Wayne
Publsiher: Academic Press
Total Pages: 366
Release: 2013-12-16
ISBN 10: 0124115365
ISBN 13: 9780124115361
Language: EN, FR, DE, ES & NL

Light and Video Microscopy Book Review:

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope Clearly develops all ideas from historical and logical foundations Laboratory exercises included to assist the reader with practical applications Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Advanced Fluorescence Microscopy

Advanced Fluorescence Microscopy
Author: Peter J. Verveer
Publsiher: Humana Press
Total Pages: 294
Release: 2016-08-23
ISBN 10: 9781493942527
ISBN 13: 1493942522
Language: EN, FR, DE, ES & NL

Advanced Fluorescence Microscopy Book Review:

This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Quantitative Fluorescence Microscopy

Quantitative Fluorescence Microscopy
Author: F. W. D. Rost,Rost
Publsiher: Cambridge University Press
Total Pages: 236
Release: 1991-07-26
ISBN 10: 9780521394222
ISBN 13: 0521394228
Language: EN, FR, DE, ES & NL

Quantitative Fluorescence Microscopy Book Review:

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.