Experiments in the Purification and Characterization of Enzymes

Experiments in the Purification and Characterization of Enzymes
Author: Thomas E. Crowley,Jack Kyte
Publsiher: Unknown
Total Pages: 266
Release: 2014-02-27
ISBN 10: 9780124095441
ISBN 13: 0124095445
Language: EN, FR, DE, ES & NL

Experiments in the Purification and Characterization of Enzymes Book Review:

Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. • Offers project lab formats for students that closely simulate original research projects • Provides instructional guidance for students to design their own experiments • Includes advanced analytical techniques • Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH. • Includes access to a website with additional resources for instructors.

Experiments in the Purification and Characterization of Enzymes

Experiments in the Purification and Characterization of Enzymes
Author: Thomas E. Crowley,Jack Kyte
Publsiher: Academic Press
Total Pages: 266
Release: 2014-01-11
ISBN 10: 0124095933
ISBN 13: 9780124095939
Language: EN, FR, DE, ES & NL

Experiments in the Purification and Characterization of Enzymes Book Review:

Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Offers project lab formats for students that closely simulate original research projects Provides instructional guidance for students to design their own experiments Includes advanced analytical techniques Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH Includes access to a website with additional resources for instructors

Purification and Characterization of Secondary Metabolites

Purification and Characterization of Secondary Metabolites
Author: Thomas E. Crowley
Publsiher: Academic Press
Total Pages: 250
Release: 2019-09
ISBN 10: 9780128139424
ISBN 13: 0128139420
Language: EN, FR, DE, ES & NL

Purification and Characterization of Secondary Metabolites Book Review:

Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical Biochemistry provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Presented in this manual is an overview of the microbiological and biochemical methods that are typically used for purification of metabolites. It discussed the biological significance of secondary metabolites that are secreted by three diverse species of bacteria. These molecules function in cell to cell signaling, acquisition of iron and phytotoxicity. Included in this manual are instruction and experiments that involve purification and characterization of enzymes from various source materials giving students excellent experience kinetics analysis and data analysis opportunities. Additionally, this lab manual covers. The theory and practice of the most commonly-used techniques of analytical biochemistry, UV-vis and IR spectrophotometry, high-performance liquid chromatography, mass spectrometry, X-ray crystallography and nuclear magnetic resonance and how to evaluate and effectively use scientific data. For each of the three metabolites, a set of exercises with detailed protocols, is included. In these exercises: 1) cultures of bacteria are grown, 2) the metabolite is purified and 3) the metabolite is analyzed with various physical and chemical techniques. Students are also given guidance in designing their own experiments to characterize secondary bacterial metabolites having features distinct from those of the metabolites addressed. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises. Written in a logical, easy-to-understand manner, Purification and Characterization of Secondary Metabolites: A Laboratory Manual for Analytical Biochemistry is an indispensible resource for both students and instructors in the fields of biochemistry molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics.

Principles and Reactions of Protein Extraction Purification and Characterization

Principles and Reactions of Protein Extraction  Purification  and Characterization
Author: Hafiz Ahmed,Hafiz Ahmed PhD
Publsiher: CRC Press
Total Pages: 408
Release: 2017-07-27
ISBN 10: 9780203507438
ISBN 13: 0203507436
Language: EN, FR, DE, ES & NL

Principles and Reactions of Protein Extraction Purification and Characterization Book Review:

Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different

Strategies for Protein Purification and Characterization

Strategies for Protein Purification and Characterization
Author: Daniel R. Marshak,Richard R. Burgess,Mark W. Knuth
Publsiher: CSHL Press
Total Pages: 396
Release: 1996
ISBN 10: 9780879693855
ISBN 13: 0879693851
Language: EN, FR, DE, ES & NL

Strategies for Protein Purification and Characterization Book Review:

Cold Spring Harbor Laboratory. Softcover manual of fundamental procedures commonly used in protein biochemistry, for reseachers. Plastic comb spiral binding.

The Purification and Characterization of ATP RNA Adenylytransferase from Pseudomonas Putida

The Purification and Characterization of ATP RNA Adenylytransferase from Pseudomonas Putida
Author: Kenneth John Payne
Publsiher: Unknown
Total Pages: 132
Release: 1969
ISBN 10:
ISBN 13: MSU:31293031428273
Language: EN, FR, DE, ES & NL

The Purification and Characterization of ATP RNA Adenylytransferase from Pseudomonas Putida Book Review:

Purification and Characterization of Cystine Lyase from Turnip Brassica Rapa and Its Possible Identity with B cystathionase

Purification and Characterization of Cystine Lyase from Turnip  Brassica Rapa  and Its Possible Identity with B cystathionase
Author: Neal Warren Anderson
Publsiher: Unknown
Total Pages: 329
Release: 1974
ISBN 10:
ISBN 13: CORNELL:31924001715105
Language: EN, FR, DE, ES & NL

Purification and Characterization of Cystine Lyase from Turnip Brassica Rapa and Its Possible Identity with B cystathionase Book Review:

Production Partial Purification and Characterization of Lipolytic Enzyme from Trichoderma Koningii

Production  Partial Purification  and Characterization of Lipolytic Enzyme from Trichoderma Koningii
Author: Tzu-Yang Huang
Publsiher: Unknown
Total Pages: 256
Release: 1995
ISBN 10:
ISBN 13: CORNELL:31924073134870
Language: EN, FR, DE, ES & NL

Production Partial Purification and Characterization of Lipolytic Enzyme from Trichoderma Koningii Book Review:

Biochemistry Laboratory

Biochemistry Laboratory
Author: Rodney F. Boyer
Publsiher: Pearson College Division
Total Pages: 380
Release: 2006
ISBN 10: 9780805346138
ISBN 13: 0805346139
Language: EN, FR, DE, ES & NL

Biochemistry Laboratory Book Review:

KEY BENEFIT: Many biochemistry lab instructors are now opting to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. Part I: Theory and Experimental Techniques, Introduction to the Biochemistry Laboratory, The Computer as a Tool in Biochemistry and Molecular Biology, General Laboratory Procedures, Centrifugation Techniques in Biochemistry, Purification and Identification of Biomolecules by Chromatography, Characterization of Proteins and Nucleic Acids by Electrophoresis, Spectroscopic Analysis of Biomolecules, Biomolecular Interactions: Ligand Binding and Enzyme Reactions, Molecular Biology I: Structures and Analysis of Nucleic Acids, Molecular Biology II: Recombinant DNA. Molecular Cloning, and Enzymology, Protein Production, Purification, and Characterization, Part II: Teaching the Biochemistry/Molecular Biology Lab, A Brief History, A Variety of Teaching Methods, Essential BMB Concepts and Skills for Student Learning, Experiments in Biochemistry and Molecular Biology KEY MARKET: For all readers interested in laboratory experiments.

Production Purification and Characterization of Thermostable Protease from Alkaliphilic and Thermophilic

Production  Purification and Characterization of Thermostable Protease from Alkaliphilic and Thermophilic
Author: Seden Güracar,İzmir Yüksek Teknoloji Enstitüsü
Publsiher: Unknown
Total Pages: 152
Release: 2011
ISBN 10:
ISBN 13: OCLC:769186357
Language: EN, FR, DE, ES & NL

Production Purification and Characterization of Thermostable Protease from Alkaliphilic and Thermophilic Book Review:

Proteases are the hydrolase enzymes that catalyze the hydrolysis of the peptide bonds in the primary structure of proteins and peptids. They are used to cleave the proteins specifically to produce useful peptides in the processes. Proteases are present in a wide variety of living organisms and they also show different physicological, physicochemical, biological, chemical functions on the earth. They are the most important enzymes in the industry, accounting for 60% of the total enzyme scales in the world. The microorganisms that were previously isolated and characterized as a Bacillus sp. from Balçova Geotermal region in İzmir were used in the experiments. The aim of this study was to produce the protease enzyme from alkaliphilic and thermophilic Bacillus sp., purify and determine the properties of the enzyme with the characterization steps. When the screening studies and growth conditions were investigated, it was understood that the alkaliphilic and thermophilic Bacillus sp. produced extracellular protease enzyme. This extracellular protease enzyme was purified by ammonium sulphate precipitation and ion exchange chromatography chromatograpy. The yield and purification fold after purification of the enzyme were 33% and 1.41, respectively. In the characterization studies, the results indicated that the protease enzyme had highest activity at pH 8.0 and 55 C. The protease enzyme lost 20% of its activity at pH 4.0 and it lost 10% of its activity at pH 10.0. The protease enzyme at temperatures below 55 C lost 15% of its activity and also the protease enzyme at temperatures above 55 C lost 25% of its activity. The protease enzyme was stable at different pH values during 3 hours and at different temperature values during 6 hours. When compared the substrates, casein showed higher activity. The effect of organic solvents and surfactants on protease activity was investigated and the results indicated that the protease enzyme was stable in the presence of 10% of the organic solvents and 1% of the surfactants. PMSF and the protease inhibitor coctail decrease the activity of the protease.

Hatching Enzyme in the Sea Urchin Strongylocentrotus Purpuratus Purification Characterization and Time of Synthesis

Hatching Enzyme in the Sea Urchin Strongylocentrotus Purpuratus  Purification  Characterization  and Time of Synthesis
Author: Benjamin Frank Edwards
Publsiher: Unknown
Total Pages: 232
Release: 1978
ISBN 10:
ISBN 13: UCAL:X14092
Language: EN, FR, DE, ES & NL

Hatching Enzyme in the Sea Urchin Strongylocentrotus Purpuratus Purification Characterization and Time of Synthesis Book Review:

The Purification and Characterization of a Nuclease from the Seeds of Muskmelon

The Purification and Characterization of a Nuclease from the Seeds of Muskmelon
Author: Lawrence David Muschek
Publsiher: Unknown
Total Pages: 302
Release: 1970
ISBN 10:
ISBN 13: MSU:31293017299607
Language: EN, FR, DE, ES & NL

The Purification and Characterization of a Nuclease from the Seeds of Muskmelon Book Review:

Purification and Characterization of Several Components of the RNA Editing Machinery from Leishmania Tarentolae Mitochondria

Purification and Characterization of Several Components of the RNA Editing Machinery from Leishmania Tarentolae Mitochondria
Author: Marian Joann Peris
Publsiher: Unknown
Total Pages: 418
Release: 1998
ISBN 10:
ISBN 13: UCLA:L0078728854
Language: EN, FR, DE, ES & NL

Purification and Characterization of Several Components of the RNA Editing Machinery from Leishmania Tarentolae Mitochondria Book Review:

Purification and Characterization of Cystine Lyase in Broccoli

Purification and Characterization of Cystine Lyase in Broccoli
Author: Edna Cristina Ramirez Valdes
Publsiher: Unknown
Total Pages: 314
Release: 1997
ISBN 10:
ISBN 13: UCAL:X60354
Language: EN, FR, DE, ES & NL

Purification and Characterization of Cystine Lyase in Broccoli Book Review:

Partial Purification and Characterization of the Enzyme Trimethylamine Oxide Demethylase from Whiting Merlangius Merlangus

Partial Purification and Characterization of the Enzyme Trimethylamine Oxide Demethylase from Whiting  Merlangius Merlangus
Author: Wiebke Havemeister
Publsiher: Unknown
Total Pages: 160
Release: 2000
ISBN 10:
ISBN 13: UCSD:31822029806379
Language: EN, FR, DE, ES & NL

Partial Purification and Characterization of the Enzyme Trimethylamine Oxide Demethylase from Whiting Merlangius Merlangus Book Review:

Purification and Characterization of an ga s ketoisocaproate Oxidase from Rat Liver

Purification and Characterization of an    ga   s ketoisocaproate Oxidase from Rat Liver
Author: Patrick John Sabourin
Publsiher: Unknown
Total Pages: 266
Release: 1981
ISBN 10:
ISBN 13: MSU:31293031691862
Language: EN, FR, DE, ES & NL

Purification and Characterization of an ga s ketoisocaproate Oxidase from Rat Liver Book Review:

Partial Purification and Characterization of Invertase and Its Proteinaceous Inhibitor from Potato Tubers

Partial Purification and Characterization of Invertase and Its Proteinaceous Inhibitor from Potato Tubers
Author: Roger Steven Anderson
Publsiher: Unknown
Total Pages: 278
Release: 1978
ISBN 10:
ISBN 13: CORNELL:31924001696545
Language: EN, FR, DE, ES & NL

Partial Purification and Characterization of Invertase and Its Proteinaceous Inhibitor from Potato Tubers Book Review:

Purification and Characterization of Nitrate Reductase

Purification and Characterization of Nitrate Reductase
Author: Karen Lund Sharp
Publsiher: Unknown
Total Pages: 272
Release: 1974
ISBN 10:
ISBN 13: UCSD:31822003778123
Language: EN, FR, DE, ES & NL

Purification and Characterization of Nitrate Reductase Book Review:

The Purification and Characterization of Lysophospholipases from the Macrophage like Cell Line P388D1

The Purification and Characterization of Lysophospholipases from the Macrophage like Cell Line P388D1
Author: Ying Yi Zhang
Publsiher: Unknown
Total Pages: 378
Release: 1989
ISBN 10:
ISBN 13: UCSD:31822003987567
Language: EN, FR, DE, ES & NL

The Purification and Characterization of Lysophospholipases from the Macrophage like Cell Line P388D1 Book Review:

Purification and Characterization of an Acyl coenzyme a Thioesterase Activity Associated with Yeast Mitochondrial ATPase

Purification and Characterization of an Acyl coenzyme a Thioesterase Activity Associated with Yeast Mitochondrial ATPase
Author: Robert Joseph Stack
Publsiher: Unknown
Total Pages: 178
Release: 1983
ISBN 10:
ISBN 13: UCAL:X16338
Language: EN, FR, DE, ES & NL

Purification and Characterization of an Acyl coenzyme a Thioesterase Activity Associated with Yeast Mitochondrial ATPase Book Review: