Essentials of 3D Biofabrication and Translation

Essentials of 3D Biofabrication and Translation discusses the techniques that are making bioprinting a viable alternative in regenerative medicine. The book runs the gamut of topics related to the subject, including hydrogels and polymers, nanotechnology, toxicity testing, and drug screening platforms, also introducing current applications in the cardiac, skeletal, and nervous systems, and organ construction. Leaders in clinical medicine and translational science provide a global perspective of the transformative nature of this field, including the use of cells, biomaterials, and macromolecules to create basic building blocks of tissues and organs, all of which are driving the field of biofabrication to transform regenerative medicine. Provides a new and versatile method to fabricating living tissue Discusses future applications for 3D bioprinting technologies, including use in the cardiac, skeletal, and nervous systems, and organ construction Describes current approaches and future challenges for translational science Runs the gamut of topics related to the subject, from hydrogels and polymers to nanotechnology, toxicity testing, and drug screening platforms

3D tissue modelling is an emerging field used for the investigation of disease mechanisms and drug development. The two key drivers of this upsurge in research lie in its potential to offer a way to reduce animal testing with respect to biotoxicity analysis, preferably on physiology recapitulated human tissues and, additionally, it provides an alternative approach to regenerative medicine. Integrating physics, chemistry, materials science, and stem cell and biomedical engineering, this book provides a complete foundation to this exciting, and interdisciplinary field. Beginning with the basic principles of 3D tissue modelling, the reader will find expert reviews on key fabrication technologies and processes, including microfluidics, microfabrication technology such as 3D bioprinting, and programming approaches to emulating human tissue complexity. The next stage introduces the reader to a range of materials used for 3D tissue modelling, from synthetic to natural materials, as well as the emerging field of tissue derived decellularized extracellular matrix (dECM). A whole host of critical applications are covered, with several chapters dedicated to hard and soft tissues, as well as focused reviews on the respiratory and central nervous system. Finally, the development of in vitro tissue models to screen drugs and study progression and etiologies of diseases, with particular attention paid to cancer, can be found.

3D Printing Technology in Nanomedicine provides an integrated and introductory look into the rapidly evolving field of nanobiotechnology. It demystifies the processes of commercialization and discusses legal and regulatory considerations. With a focus on nanoscale processes and biomedical applications, users will find this to be a comprehensive resource on how 3D printing can be utilized in a range of areas, including the diagnosis and treatment of a variety of human diseases. Examines the emerging market of 3D-printed biomaterials and their clinical applications, with a particular focus on both commercial and premarket tools Examines the promising market of 3D-printed nanoparticles, nanomaterial, biomaterials, composite nanomaterial and their clinical applications in the cardiovascular and chemotherapy realms Develops the concept of integrating different technologies along the hierarchical structure of biological systems

This book gathers the joint proceedings of the VIII Latin American Conference on Biomedical Engineering (CLAIB 2019) and the XLII National Conference on Biomedical Engineering (CNIB 2019). It reports on the latest findings and technological outcomes in the biomedical engineering field. Topics include: biomedical signal and image processing; biosensors, bioinstrumentation and micro-nanotechnologies; biomaterials and tissue engineering. Advances in biomechanics, biorobotics, neurorehabilitation, medical physics and clinical engineering are also discussed. A special emphasis is given to practice-oriented research and to the implementation of new technologies in clinical settings. The book provides academics and professionals with extensive knowledge on and a timely snapshot of cutting-edge research and developments in the field of biomedical engineering.

Mechanics of Biological Systems and Materials & Micro-and Nanomechanics, Volume 4 of the Proceedings of the 2019 SEM Annual Conference & Exposition on Experimental and Applied Mechanics, the fourth volume of six from the Conference, brings together contributions to important areas of research and engineering. The collection presents early findings and case studies on a wide range of topics, including: Extreme NanomechanicsIn-Situ NanomechanicsExpanding Boundaries in MetrologyMicro and Nanoscale DeformationMEMS for Actuation, Sensing and Characterization1D & 2D MaterialsCardiac MechanicsCell Mechanics Biofilms and Microbe MechanicsTraumatic Brain InjuryOrthopedic BiomechanicsLigaments and Soft Materials

Biofabrication is a practical guide to the novel, inherently cross-disciplinary scientific field that focuses on biomanufacturing processes and a related range of emerging technologies. These processes and technologies ultimately further the development of products that may involve living (cells and/or tissues) and nonliving (bio-supportive proteins, scaffolds) components. The book introduces readers to cell printing, patterning, assembling, 3D scaffold fabrication, cell/tissue-on-chips as a coherent micro-/nano-fabrication toolkit. Real-world examples illustrate how to apply biofabrication techniques in areas such as regenerative medicine, pharmaceuticals and tissue engineering. In addition to being a vital reference for scientists, engineers and technicians seeking to apply biofabrication techniques, this book also provides an insight into future developments in the field, and potential new applications. Discover the multi-disciplinary toolkit provided by biofabrication and apply it to develop new products, techniques and therapies Covers a range of important emerging technologies in a coherent manner: cell printing, patterning, assembling, 3D scaffold fabrication, cell/tissue-on-chips... Readers develop the ability to apply biofabrication technologies through practical examples

This volume provides an in-depth introduction to 3D printing and biofabrication and covers the recent advances in additive manufacturing for tissue engineering. The book is divided into two parts, the first part on 3D printing discusses conventional approaches in additive manufacturing aimed at fabrication of structures, which are seeded with cells in a subsequent step. The second part on biofabrication presents processes which integrate living cells into the fabrication process.

Virtually any disease that results from malfunctioning, damaged, or failing tissues may be potentially cured through regenerative medicine therapies, by either regenerating the damaged tissues in vivo, or by growing the tissues and organs in vitro and implanting them into the patient. Principles of Regenerative Medicine discusses the latest advances in technology and medicine for replacing tissues and organs damaged by disease and of developing therapies for previously untreatable conditions, such as diabetes, heart disease, liver disease, and renal failure. Key for all researchers and instituions in Stem Cell Biology, Bioengineering, and Developmental Biology The first of its kind to offer an advanced understanding of the latest technologies in regenerative medicine New discoveries from leading researchers on restoration of diseased tissues and organs

3D Printing in Medicine examines the emerging market of 3D-printed biomaterials and its clinical applications. With a particular focus on both commercial and premarket tools, the book looks at their applications within medicine and the future outlook for the field. The book begins with a discussion of the fundamentals of 3D printing, including topics such as materials, and hardware. Chapters go on to cover applications within medicine such as computational analysis of 3D printed constructs, personalized 3D printing and 3D cell and organ printing. The concluding chapters in the book review the applications of 3D printing in diagnostics, drug development, 3D-printed disease models and 3D printers for surgical practice. With a strong focus on the translation of 3D printing technology to a clinical setting, this book is a valuable resource for scientists and engineers working in biomaterial, biomedical, and nanotechnology based industries and academia. Provides a comprehensive and authoritative overview of all the medical applications of 3D printing biomaterials and technologies Focuses on the emerging market of 3D printed biomaterials in clinical applications Reviews both commercial and under development materials, tools, their applications, and future evolution

Laser-assisted bioprinting (LAB) is an emerging technology in the field of tissue engineering. Its physical mechanism makes it possible to print cells and liquid materials with a cell-level resolution. By giving tissue engineers control over cell density and organization of 3D tissue constructs, LAB holds much promise for fabricating living tissues with physiological functionality. After introducing the rationale of applying LAB to tissue engineering, we present exhaustively the physical parameters related to the laser-induced forward transfer technique (LIFT), which is implemented in LAB. These parameters are critical to controlling the cell printing process and must work together to print viable cell patterns with respect to cell-level histological organization and to high-throughput manufacturing. After describing the experimental requirements that should be considered to fabricate 3D tissues by LAB, we present some of the main breakthroughs, including multicomponent printing, 3D printing approaches, and bioprinting in vivo that may serve in tissue engineering and regenerative medicine.

Much research has focused on the basic cellular and molecular biological aspects of stem cells. Much of this research has been fueled by their potential for use in regenerative medicine applications, which has in turn spurred growing numbers of translational and clinical studies. However, more work is needed if the potential is to be realized for improvement of the lives and well-being of patients with numerous diseases and conditions. This book series 'Cell Biology and Translational Medicine (CBTMED)' as part of SpringerNature’s longstanding and very successful Advances in Experimental Medicine and Biology book series, has the goal to accelerate advances by timely information exchange. Emerging areas of regenerative medicine and translational aspects of stem cells are covered in each volume. Outstanding researchers are recruited to highlight developments and remaining challenges in both the basic research and clinical arenas. This current book is the fourth volume of a continuing series.

This book is comprehensive in nature with contributions by leading world experts in 3D bioprinting related to regenerative engineering. It includes history, incorporating the process and methods used in bioprinting. Significant sections will be reserved for the applications of the types of tissues generated by using bioprinting, along with an overview of different technologies used in bioprinting. In addition to equipment, the book also describes the different biomaterials and cells used in these approaches. Overall this is a book that includes both entry-level knowledge and advanced methods and techniques. Applications will emphasize engineering and clinical principles.

This book provides current and emerging developments in bioprinting with respect to bioprinting technologies, bioinks, applications, and regulatory pathways. Topics covered include 3D bioprinting technologies, materials such as bioinks and bioink design, applications of bioprinting complex tissues, tissue and disease models, vasculature, and musculoskeletal tissue. The final chapter is devoted to clinical applications of bioprinting, including the safety, ethical, and regulatory aspects. This book serves as a go-to reference on bioprinting and is ideal for students, researchers and professionals, including those in academia, government, the medical industry, and healthcare.

Omics Technologies and Bio-Engineering: Towards Improving Quality of Life, Volume 1 is a unique reference that brings together multiple perspectives on omics research, providing in-depth analysis and insights from an international team of authors. The book delivers pivotal information that will inform and improve medical and biological research by helping readers gain more direct access to analytic data, an increased understanding on data evaluation, and a comprehensive picture on how to use omics data in molecular biology, biotechnology and human health care. Covers various aspects of biotechnology and bio-engineering using omics technologies Focuses on the latest developments in the field, including biofuel technologies Provides key insights into omics approaches in personalized and precision medicine Provides a complete picture on how one can utilize omics data in molecular biology, biotechnology and human health care

Mathematica by Example, Revised Edition presents the commands and applications of Mathematica, a system for doing mathematics on a computer. This text serves as a guide to beginning users of Mathematica and users who do not intend to take advantage of the more specialized applications of Mathematica. The book combines symbolic manipulation, numerical mathematics, outstanding graphics, and a sophisticated programming language. It is comprised of 7 chapters. Chapter 1 gives a brief background of the software and how to install it in the computer. Chapter 2 introduces the essential commands of Mathematica. Basic operations on numbers, expressions, and functions are introduced and discussed. Chapter 3 provides Mathematica's built-in calculus commands. The fourth chapter presents elementary operations on lists and tables. This chapter is a prerequisite for Chapter 5 which discusses nested lists and tables in detail. The purpose of Chapter 6 is to illustrate various computations Mathematica can perform when solving differential equations. Chapter 7 discusses some of the more frequently used commands contained in various graphics packages available with Mathematica. Engineers, computer scientists, physical scientists, mathematicians, business professionals, and students will find the book useful.

This book offers readers a valuable overview of recent advances in biomedical engineering, as applied to the modern dentistry. It begins by studying the biomaterials in dentistry, and materials used intraoperatively during oral and maxillofacial surgery procedures. Next, it considers the subjects in which biomedical engineers can be influential, such as 3-dimensional (3D) imaging, laser and photobiomodulation, surface modification of dental implants, and bioreactors. Hard and soft tissue engineerings in dentistry are discussed, and some specific and essential methods such as 3D-printing are elaborated. Presenting particular clinical functions of regenerative dentistry and tissue engineering in treatment of oral and maxillofacial soft tissues is the subject of a separate chapter. Challenges in the rehabilitation handling of large and localized oral and maxillofacial defects is a severe issue in dentistry, which are considered to understand how bioengineers help with treatment methods in this regard. Recent advances in nanodentistry is discussed followed by a chapter on the applications of stem cell-encapsulated hydrogel in dentistry.Periodontal regeneration is a challenging issue in dentistry, and thus, is going to be considered separately to understand the efforts and achievements of tissue engineers in this matter. Oral mucosa grafting is a practical approach in engineering and treatment of tissues in ophthalmology, which is the subject of another chapter. Microfluidic approaches became more popular in biomedical engineering during the last decade; hence, one chapter focuses on the advanced topic of microfluidics technologies using oral factors as saliva-based studies. Injectable gels in endodontics is a new theme in dentistry that bioengineering skills can advance its development, specifically by producing clinically safe and effective gels with regeneration and antibacterial properties. Engineered products often need to be tested in vivo before being clinical in dentistry; thus, one chapter is dedicated to reviewing applicable animal models in dental research. The last chapter covers the progress on the whole tooth bioengineering as a valuable and ultimate goal of many dental researchers. Offers readers an interdisciplinary approach that relates biomedical engineering and restorative dentistry Discusses recent technological achievements in engineering with applications in dentistry Provides useful tool to dental companies for future product planning, specifically to biomedical engineers engaged in dental research

Regenerative Engineering and Developmental Biology: Principles and Applications examines cutting-edge developments in the field of regenerative engineering. Specific attention is given to activities that embrace the importance of integrating developmental biology and tissue engineering, and how this can move beyond repairing damage to body parts to instead regenerate tissues and organs. The text furthermore focusses on the five legs of the field of regenerative engineering, including: materials, developmental biology, stem cells, physics, and clinical translation. This book was written by leading developmental biologists; each chapter examines the processes that these biologists study and how they can be advanced by using the tools available in tissue engineering/biomaterials. Individual chapters are complete with concluding remarks and thoughts on the future of regenerative engineering. A list of references is also provided to aid the reader with further research. Ultimately, this book achieves two goals. The first encourages the biomedical community to think about how inducing regeneration is an engineering problem. The second goal highlights the discoveries with animal regeneration and how these processes can be engineered to regenerate body parts. Regenerative Engineering and Developmental Biology: Principles and Applications was written with undergraduate and graduate-level biomedical engineering students and biomedical professionals in mind.

Skin Tissue Models provides a translational link for biomedical researchers on the interdisciplinary approaches to skin regeneration. As the skin is the largest organ in the body, engineered substitutes have critical medical application to patients with disease and injury – from burn wounds and surgical scars, to vitiligo, psoriasis and even plastic surgery. This volume offers readers preliminary description of the normal structure and function of mammalian skin, exposure to clinical problems and disease, coverage of potential therapeutic molecules and testing, skin substitutes, models as study platforms of skin biology and emerging technologies. The editors have created a table of contents which frames the relevance of skin tissue models for researchers as platforms to study skin biology and therapeutic approaches for different skin diseases, for clinicians as tissue substitutes, and for cosmetic and pharmaceutical industries as alternative test substrates that can replace animal models. Offers descriptions of the normal structure/function of mammalian skin, exposure to clinical problems, and more Presents coverage of skin diseases (cancer, genodermatoses, vitiligo and psoriasis) that extends to clinical requirements and skin diseases in vitro models Addresses legal requirements and ethical concerns in drugs and cosmetics in vitro testing Edited and authored by internationally renowned group of researchers, presenting the broadest coverage possible

Spinal Cord Injury (SCI) Repair Strategies provides researchers the latest information on potential regenerative approaches to spinal cord injury, specifically focusing on therapeutic approaches that target regeneration, including cell therapies, controlled drug delivery systems, and biomaterials. Dr. Giuseppe Perale and Dr. Filippo Rossi lead a team of authoritative authors in academia and industry in this innovative reference on the field of regenerative medicine and tissue engineering. This book presents all the information readers need to understand the current and potential array of techniques, materials, applications and their benefits for spinal cord repair. Covers current and future repair strategies for spinal cord injury repair Focuses on key research trends, clinics, biology and engineering Provides fundamentals on regenerative engineering and tissue engineering

The book outlines first the importance of Extra Cellular Matrix (ECM), which is a natural surface for most of cells. In the following chapters the influence of biological, chemical, mechanical, and physical properties of surfaces in micro and nano-scale on stem cell behavior are discussed including the mechanotransduction. Biomimetic and bioinspired approaches are highlighted for developing microenvironment of several tissues, and surface engineering applications are discussed in tissue engineering, regenerative medicine and different type of biomaterials in various chapters of the book. This book brings together innovative methodologies and strategies adopted in the research and development of Advanced Surfaces in Stem Cell Research. Well-known worldwide researchers deliberate subjects including: Extracellular matrix proteins for stem cell fate The superficial mechanical and physical properties of matrix microenvironment as stem cell fate regulator Effects of mechanotransduction on stem cell behavior Modulation of stem cells behavior through bioactive surfaces Influence of controlled micro and nanoengineered surfaces on stem cell fate Nanostructured polymeric surfaces for stem cells Laser surface modification techniques and stem cells applications Plasma polymer deposition: a versatile tool for stem cell research Application of bioreactor concept and modeling techniques in bone regeneration and augmentation treatments Substrates and surfaces for control of pluripotent stem cell fate and function Application of biopolymer-based, surface modified devices in transplant medicine and tissue engineering Silk as a natural biopolymer for tissue engineering