Principles of Enzyme Kinetics

Principles of Enzyme Kinetics
Author: Athel Cornish-Bowden
Publsiher: Elsevier
Total Pages: 220
Release: 2014-05-20
ISBN 10: 1483164675
ISBN 13: 9781483164670
Language: EN, FR, DE, ES & NL

Principles of Enzyme Kinetics Book Review:

Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Essential Enzyme Kinetics

Essential Enzyme Kinetics
Author: Daniel L. Purich
Publsiher: Academic Press
Total Pages: 425
Release: 2020-06-01
ISBN 10: 0128218096
ISBN 13: 9780128218099
Language: EN, FR, DE, ES & NL

Essential Enzyme Kinetics Book Review:

Essential Enzyme Kinetics: A Textbook for Molecular Life Scientists describes the theoretical basis and best-practice approaches for using initial-rate, fast reaction, and kinetic isotope effect experiments to define enzyme catalysis. Because a detailed knowledge of enzyme transition-states is the main driver for the rational design of slow, tight-binding inhibitors destined to become tomorrow's small-molecule drugs, Essential Enzyme Kinetics is the must-have reference for chemists, biochemists, and pharmacologists intent on pursuing careers in Big Pharma. Given the interdisciplinary nature of contemporary drug development, this book provides a lucid short-course that will also benefit nonspecialists seeking to understand the scope and reach of modern enzyme kinetics. Provides practical information about how to work with enzymes and design experiments to identify new inhibitors or activators Includes detailed step-by-step derivations of rate equations, showing tried-and-true ways to confirm that the equations obtained are correct Arranged for use both as a desk reference (with over 200 equations and more than 400 key references) or as a textbook (with 8-10 problems/exercises at the end of each chapter)

Fundamentals of Enzyme Kinetics

Fundamentals of Enzyme Kinetics
Author: Athel Cornish-Bowden
Publsiher: John Wiley & Sons
Total Pages: 510
Release: 2013-02-22
ISBN 10: 3527665498
ISBN 13: 9783527665495
Language: EN, FR, DE, ES & NL

Fundamentals of Enzyme Kinetics Book Review:

Now in its fourth edition, this textbook is one of the few titles worldwide to cover enzyme kinetics in its entire scope and the only one to include its implications for bioinformatics and systems biology. Multi-enzyme complexes and cooperativity are therefore treated in more detail than in any other textbook on the market. The respected and well known author is one of the most experienced researchers into the topic and writes with outstanding style and didactic clarity. As with the previous editions, he presents here steady-state kinetics and fast reactions, supplementing each chapter with problems and solutions. For the first time, this edition features a companion website providing all figures in colour www.wiley-vch.de/home/fundenzykinet

Enzyme Kinetics

Enzyme Kinetics
Author: Paul C. Engel
Publsiher: Springer
Total Pages: 96
Release: 1981
ISBN 10: 1928374650XXX
ISBN 13: UOM:39015009028864
Language: EN, FR, DE, ES & NL

Enzyme Kinetics Book Review:

The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the fron tiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably concerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory text books or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce abrief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology.

Enzyme Kinetics

Enzyme Kinetics
Author: Alejandro G. Marangoni
Publsiher: John Wiley & Sons
Total Pages: 248
Release: 2003-04-23
ISBN 10: 0471461415
ISBN 13: 9780471461418
Language: EN, FR, DE, ES & NL

Enzyme Kinetics Book Review:

Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.

Kinetics of Enzyme Modifier Interactions

Kinetics of Enzyme Modifier Interactions
Author: Antonio Baici
Publsiher: Springer
Total Pages: 489
Release: 2015-06-24
ISBN 10: 3709114020
ISBN 13: 9783709114025
Language: EN, FR, DE, ES & NL

Kinetics of Enzyme Modifier Interactions Book Review:

The kinetic mechanisms by which enzymes interact with inhibitors and activators, collectively called modifiers, are scrutinized and ranked taxonomically into autonomous species in a way similar to that used in the biological classification of plants and animals. The systematization of the mechanisms is based on two fundamental characters: the allosteric linkage between substrate and modifier and the factor by which a modifier affects the catalytic constant of the enzyme. Combinations of the physically significant states of these two characters in an ancestor-descendant-like fashion reveal the existence of seventeen modes of interaction that cover the needs of total, partial and fine-tuning modulation of enzyme activity. These interactions comprise five linear and five hyperbolic inhibition mechanisms, five nonessential activation mechanisms and two hybrid species that manifest either hyperbolic inhibition or nonessential activation characteristics depending on substrate concentration. Five essential activation mechanisms, which are taxonomically independent of the mentioned basic species, complete the inventory of enzyme modifiers. Often masked under conventional umbrella terms or treated as anomalous cases, all seventeen basic inhibition and nonessential activation mechanisms are represented in the biochemical and pharmacological literature of this and the past century, either in the form of rapid or slow-onset reversible interactions, or as irreversible modification processes. The full potential of enzyme inhibitors and activators can only be appreciated after elucidating the details of their kinetic mechanisms of action exploring the entire range of physiologically significant reactant concentrations. This book highlights the wide spectrum of allosteric enzyme modification in physiological occurrences as well as in pharmacological and biotechnological applications that embrace simple and multiple enzyme-modifier interactions. The reader is guided in the journey through this still partly uncharted territory with the aid of mechanistically-oriented criteria aimed at showing the logical way towards the identification of a particular mechanism.

Kinetics of Enzyme Action

Kinetics of Enzyme Action
Author: Ross L. Stein
Publsiher: John Wiley & Sons
Total Pages: 320
Release: 2011-08-08
ISBN 10: 9781118084403
ISBN 13: 1118084403
Language: EN, FR, DE, ES & NL

Kinetics of Enzyme Action Book Review:

Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.

Enzyme Kinetics Catalysis and Control

Enzyme Kinetics  Catalysis and Control
Author: Daniel L. Purich
Publsiher: Elsevier
Total Pages: 920
Release: 2010-06-16
ISBN 10: 0123809258
ISBN 13: 9780123809254
Language: EN, FR, DE, ES & NL

Enzyme Kinetics Catalysis and Control Book Review:

Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions

Contemporary Enzyme Kinetics and Mechanism

Contemporary Enzyme Kinetics and Mechanism
Author: Daniel L. Purich
Publsiher: Academic Press
Total Pages: 566
Release: 1983-01-01
ISBN 10: 1483214222
ISBN 13: 9781483214221
Language: EN, FR, DE, ES & NL

Contemporary Enzyme Kinetics and Mechanism Book Review:

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Enzyme Kinetics

Enzyme Kinetics
Author: Hans Bisswanger
Publsiher: John Wiley & Sons
Total Pages: 320
Release: 2017-06-22
ISBN 10: 3527806474
ISBN 13: 9783527806478
Language: EN, FR, DE, ES & NL

Enzyme Kinetics Book Review:

Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics. Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application. With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work.

Enzyme Kinetics in Drug Metabolism

Enzyme Kinetics in Drug Metabolism
Author: Swati Nagar,Upendra Argikar,Donald Tweedie
Publsiher: Humana Press
Total Pages: 538
Release: 2014-02-13
ISBN 10: 9781627037570
ISBN 13: 1627037578
Language: EN, FR, DE, ES & NL

Enzyme Kinetics in Drug Metabolism Book Review:

Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.

Enzyme Kinetics and Mechanisms

Enzyme Kinetics and Mechanisms
Author: Kenneth B. Taylor
Publsiher: Springer Science & Business Media
Total Pages: 227
Release: 2002-07-31
ISBN 10: 1402007280
ISBN 13: 9781402007286
Language: EN, FR, DE, ES & NL

Enzyme Kinetics and Mechanisms Book Review:

Enzyme Kinetics and Mechanisms takes the reader through the experimental techniques and the logic by which the mechanisms of enzyme-catalyzed reactions can be elucidated by the results of steady-state kinetics and related experiments. It is meant to make these investigations both satisfying and effective. In distinction to other available descriptions, the descriptions in enzyme Kinetics and Mechanisms are limited to more commonly utilized and useful models and techniques. The logic relating the chemical models to the mathematical models and the logic of relating the mathematical models to data is presented in rather concise text, figures and equations. The development of mathematical models from chemical models is done by a unique algorithm that is both simple and quick, and the same concept are utilized to develop models for the effects of a variety of reaction conditions on the initial velocity. In addition, the various relationships of data, mathematical models and the chemical models is illustrated with examples from the scientific literature. Enzyme Kinetics and Mechanisms is intended for research workers, graduate students, post doctoral associates, and faculty in biochemistry and molecular biology who are interested in the techniques and logic by which mechanisms of enzymes-catalyzed reactions can be elucidated by investigation of steady-state kinetic experiments.

Essentials of Enzymology

Essentials of Enzymology
Author: Rufus O. Okotore
Publsiher: Xlibris Corporation
Total Pages: 221
Release: 2015-03-13
ISBN 10: 1503527220
ISBN 13: 9781503527225
Language: EN, FR, DE, ES & NL

Essentials of Enzymology Book Review:

Essentials of Enzymology provides concise information on an important area of the subject, Biochemistry. This may serve as course material for an advanced treatise in Enzymology designed for undergraduate science degree programs, especially B.Sc. (Hons ) Biochemistry and Chemistry. The book is in 12 chapters which has been divided into four distinct sections, thus (1) Basic enzyme chemistry and physiology. (2) Enzyme Kinetics, (3) Enzyme catalysis, Mechanisms and Regulation,(4)Applications of Enzymology. The Part 1 consists of four chapters that deal with the nature of enzymes- (history, properties and classifi cation), enzyme physiology; structure of enzymes, and analytical enzymology. Part 2 deals with Enzyme Kinetics which is treated in three chapters, and Part 3, made up of three chapters discuss Enzyme catalysis, mechanisms and regulation. Lastly, Part 4 consisting of two chapters deal with the applications of enzymology. Signifi cantly, the kinetics of enzyme catalyzed reactions in diverse experimental conditions, and also under various inhibition types are presented in a simple, mathematical lucid approach. The mechanisms of action for two atypical proteins-chymotrypsin and lysozyme, so also the identifi cation of active sites of enzymes by specifi c labels are discussed concisely. Lastly, the specifi c applications of enzymes in diagnostic medicine, industry, and also the new emerging area of enzyme biotechnology and enzyme bioinformatics are presented

Enzyme Kinetics and Regulation

Enzyme Kinetics and Regulation
Author: Aaren Bennett
Publsiher: Scientific e-Resources
Total Pages: 328
Release: 2018-07-07
ISBN 10: 183947176X
ISBN 13: 9781839471766
Language: EN, FR, DE, ES & NL

Enzyme Kinetics and Regulation Book Review:

We live in the age of science-the human and numerous other living beings' genomes have been sequenced and we are beginning to understand the capacity of the metabolic machinery responsible for life on our planet. A huge number of new genes have been discovered, a significant number of these coding for enzymes of yet obscure capacity. Understanding the kinetic behavior of an enzyme provides clues to its possible physiological role. From a biotechnological perspective, knowledge of the reactant properties of an enzyme is required for the design of immobilized enzyme-based modern processes. Biotransformations are of key importance to the pharmaceutical and sustenance industries, and knowledge of the reactant properties of enzymes, essential. This book is tied in with understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the reactant capacity of an enzyme. Coverage of the material is in no way, shape or form exhaustive. There exist many books on enzyme kinetics that offer intensive, in-depth treatises of the subject. Intracellular and extracellular physiological cascades are regulated by initiation and hindrance of different enzymes involved in these pathways. Investigating and understanding the mechanism of enzyme hindrance has become the premise of development of pharmaceutical agents. Organically active regular and synthetic inhibitors have been developed and special emphasis has been placed on investigations that define their structure-work relationships in an effort to understand the inception of their natural properties. A powerful complement to the assessment of these agents is the preparation and subsequent examination of key fractional structures, deep-seated auxiliary adjustments and the corresponding unnatural enantiomers of characteristic items. We sincerely hope that this book will represent an element in the tool kit of graduate students in applied science and chemical and biochemical engineering and furthermore of undergraduate students with formal preparing in natural chemistry, biochemistry, thermodynamics and chemical reaction kinetics.

Enzymes

Enzymes
Author: Selim Kermasha,Michael N.A. Eskin
Publsiher: Academic Press
Total Pages: 328
Release: 2020-11-27
ISBN 10: 0128005076
ISBN 13: 9780128005071
Language: EN, FR, DE, ES & NL

Enzymes Book Review:

Enzymes: Novel Biotechnological Approaches for the Food Industry provides an in-depth background of the most up-to-date scientific research and information related to food biotechnology and offers a wide spectrum of biological applications. This book addresses novel biotechnological approaches for the use of enzymes in the food industry to help readers understand the potential uses of biological applications to advance research. This is an essential resource to researchers and both undergraduate and graduate students in the biotechnological industries. Provides fundamental and rigorous scientific information on enzymes Illustrates enzymes as tools to achieve value and quality to a product, either in vitro or in vivo Presents the most updated knowledge in the area of food biotechnology Demonstrates novel horizons and potential for the use of enzymes in industrial applications

Proteolytic Enzymes

Proteolytic Enzymes
Author: Erwin E. Sterchi,Walter Stöcker
Publsiher: Springer Science & Business Media
Total Pages: 366
Release: 2013-11-11
ISBN 10: 3642598161
ISBN 13: 9783642598166
Language: EN, FR, DE, ES & NL

Proteolytic Enzymes Book Review:

Following an overview on proteolytic enzyme assays, this text covers procedures on how to investigate and study proteases. It describes the use of specific restriction proteases as well as inhibitors of proteases to prevent unwanted proteolysis.

Contemporary Enzyme Kinetics and Mechanism

Contemporary Enzyme Kinetics and Mechanism
Author: Anonim
Publsiher: Academic Press
Total Pages: 704
Release: 2009-10-24
ISBN 10: 9780123847447
ISBN 13: 0123847443
Language: EN, FR, DE, ES & NL

Contemporary Enzyme Kinetics and Mechanism Book Review:

Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Enzyme Kinetics and Mechanism

Enzyme Kinetics and Mechanism
Author: Paul F. Cook,W. W. Cleland
Publsiher: Garland Science
Total Pages: 416
Release: 2007-03-06
ISBN 10: 1136844279
ISBN 13: 9781136844270
Language: EN, FR, DE, ES & NL

Enzyme Kinetics and Mechanism Book Review:

Enzyme Kinetics and Mechanism is a comprehensive textbook on steady-state enzyme kinetics. Organized according to the experimental process, the text covers kinetic mechanism, relative rates of steps along the reaction pathway, and chemical mechanism—including acid-base chemistry and transition state structure. Practical examples taken from the literature demonstrate theory throughout. The book also features numerous general experimental protocols and how-to explanations for interpreting kinetic data. Written in clear, accessible language, the book will enable graduate students well-versed in biochemistry to understand and describe data at the fundamental level. Enzymologists and molecular biologists will find the text a useful reference.

Enzyme Inhibitors and Activators

Enzyme Inhibitors and Activators
Author: Murat Şentürk
Publsiher: BoD – Books on Demand
Total Pages: 268
Release: 2017-03-29
ISBN 10: 9535130579
ISBN 13: 9789535130574
Language: EN, FR, DE, ES & NL

Enzyme Inhibitors and Activators Book Review:

Over the recent years, medicinal chemistry has become responsible for explaining interactions of chemical molecule processes such that many scientists in the life sciences from agronomy to medicine are engaged in medicinal research. This book contains an overview focusing on the research area of enzyme inhibitor and activator, enzyme-catalyzed biotransformation, usage of microbial enzymes, enzymes associated with programmed cell death, natural products as potential enzyme inhibitors, protease inhibitors from plants in insect pest management, peptidases, and renin-angiotensin system. The book provides an overview on basic issues and some of the recent developments in medicinal science and technology. Especially, emphasis is devoted to both experimental and theoretical aspect of modern medicine. The primary target audience for the book includes students, researchers, chemists, molecular biologists, medical doctors, pharmacologists, and professionals who are interested in associated areas. The textbook is written by international scientists with expertise in biochemistry, enzymology, molecular biology, and genetics, many of which are active in biochemical and pharmacological research. I would like to acknowledge the authors for their contribution to the book. We hope that the textbook will enhance the knowledge of scientists in the complexities of some medical approaches; it will stimulate both professionals and students to dedicate part of their future research in understanding relevant mechanisms and applications of pharmacology.

ENZYMES Catalysis Kinetics and Mechanisms

ENZYMES  Catalysis  Kinetics and Mechanisms
Author: N.S. Punekar
Publsiher: Springer
Total Pages: 562
Release: 2018-11-11
ISBN 10: 9811307857
ISBN 13: 9789811307850
Language: EN, FR, DE, ES & NL

ENZYMES Catalysis Kinetics and Mechanisms Book Review:

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.