Biochemical Analysis Tools

Biochemical Analysis Tools
Author: Oana-Maria Boldura,Cornel Balta,Prof Nasser Awwad
Publsiher: BoD – Books on Demand
Total Pages: 206
Release: 2020-06-24
ISBN 10: 1789848563
ISBN 13: 9781789848564
Language: EN, FR, DE, ES & NL

Biochemical Analysis Tools Book Review:

This book explores the role of nucleic acid analysis and the advances it has led to in the field of life sciences. The first section is a collection of chapters covering experimental methods used in molecular biology, the techniques adjacent to these methods, and the steps of analysis before and after obtaining raw DNA data. The second section deals with the principles of chromatography, method development, sample preparation, and industrial applications.

Preparative Chromatography for Separation of Proteins

Preparative Chromatography for Separation of Proteins
Author: Arne Staby,Anurag S. Rathore,Satinder Ahuja
Publsiher: John Wiley & Sons
Total Pages: 608
Release: 2017-03-06
ISBN 10: 1119031109
ISBN 13: 9781119031109
Language: EN, FR, DE, ES & NL

Preparative Chromatography for Separation of Proteins Book Review:

"Preparative Chromatography for Separation of Proteins addresses a wide range of the most current modeling techniques, strategies, and case studies of industrial separation of proteins and peptides to aid in the efficiency and efficacy of this broadly-used technique in the purification of biopharmaceuticals"--

Separation Methods in Drug Synthesis and Purification

Separation Methods in Drug Synthesis and Purification
Author: Klara Valko
Publsiher: Elsevier
Total Pages: 788
Release: 2020-06-19
ISBN 10: 0444640711
ISBN 13: 9780444640710
Language: EN, FR, DE, ES & NL

Separation Methods in Drug Synthesis and Purification Book Review:

Separation Methods in Drug Synthesis and Purification, Second Edition, Volume Eight, provides an updated on the analytical techniques used in drug synthesis and purification. Unlike other books on either separation science or drug synthesis, this volume combines the two to explain the basic principles and comparisons of each separation technique. New sections to this volume include enantiomer separation using capillary electrophoresis (CE) and capillary electro- chromatography, the computer simulation of chromatographic separation for accelerating method development, the application of chromatography and capillary electrophoresis used as surrogates for biological processes, and new developments in the established techniques of chromatography and preparative methods. Features descriptions and applications of all separation methods used in the pharmaceutical industry Written by the leading scientists in their respective fields, providing solutions for a wide range of industrial separation problems encountered within the pharmaceutical industry Thoroughly updated with brand new separation science techniques and the latest developments in the established techniques of chromatography

Identification and Quantification of Drugs Metabolites and Metabolizing Enzymes by LC MS

Identification and Quantification of Drugs  Metabolites and Metabolizing Enzymes by LC MS
Author: Swapan Chowdhury
Publsiher: Elsevier
Total Pages: 354
Release: 2005-11-04
ISBN 10: 9780080456065
ISBN 13: 0080456065
Language: EN, FR, DE, ES & NL

Identification and Quantification of Drugs Metabolites and Metabolizing Enzymes by LC MS Book Review:

As new techniques of transferring from liquid to gas phase and measuring masses of drug molecules and metabolites become more prevalent, so do the technical challenges of putting these techniques into proper use, as well as the task of consolidating emerging applications. Identification and Quantification of Drugs, Metabolites and Metabolizing Enzymes by LC-MS, Volume 6 fills the gap in the lack of presently available literature by providing a critical review in the current use of liquid chromatography-mass spectrometry (LC-MS) in drug discovery and development. With chapters written by experts with a wide range of practical experience from the pharmaceutical industry, emphasis is placed on techniques and applications. The book also includes chapters on how to utilize LC-MS instrumentation for current drug metabolism problems. This book is intended for those beginning to use LC-MS for drug metabolism studies as well as for those considered advanced practitioners. * Introduces readers to the practical applications of modern liquid chromatography-mass spectrometry (LC-MS) in a wide range of drug metabolism studies· * Provides a comprehensive description of different forms of metabolites, with detailed discussion on the wide range of methodologies used to identify them * Highlights problems associated with drug quantification and offers practical solutions

Handbook of Pharmaceutical Analysis

Handbook of Pharmaceutical Analysis
Author: Lena Ohannesian,Anthony Streeter
Publsiher: CRC Press
Total Pages: 608
Release: 2001-11-09
ISBN 10: 0824741943
ISBN 13: 9780824741945
Language: EN, FR, DE, ES & NL

Handbook of Pharmaceutical Analysis Book Review:

Exploring the analysis of pharmaceuticals, including polymorphic forms, this book discusses regulatory requirements in pharmaceutical product development and pharmaceutical testing. It covers methods of drug separation and procedures such as capillary electrophoresis for chromatographic separation of molecules. Additional topics include drug formulation analysis using vibrational and magnetic resonance spectroscopy and identification of drug metabolites and decomposition products using such techniques as mass spectrometry. The book provides more than 300 tables, equations, drawings, and photographs, and convenient, easy-to-use indices, facilitating quick access to each topic.

HPLC for Pharmaceutical Scientists

HPLC for Pharmaceutical Scientists
Author: Yuri V. Kazakevich,Rosario LoBrutto
Publsiher: John Wiley & Sons
Total Pages: 1080
Release: 2007-02-16
ISBN 10: 0470087943
ISBN 13: 9780470087947
Language: EN, FR, DE, ES & NL

HPLC for Pharmaceutical Scientists Book Review:

HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.

Chirality in Drug Research

Chirality in Drug Research
Author: Eric Francotte,Wolfgang Lindner
Publsiher: John Wiley & Sons
Total Pages: 370
Release: 2007-09-24
ISBN 10: 3527608710
ISBN 13: 9783527608713
Language: EN, FR, DE, ES & NL

Chirality in Drug Research Book Review:

Divided into the three main sections of synthesis, analysis and drug development, this handbook covers all stages of the drug development process, including large-scale synthesis and purification of chirally pure pharmaceuticals. The two editors from academia and a major pharmaceutical company have assembled an experienced, international team who provide first-hand practical advice and report previously unpublished data. In the first section, the isolation of chiral drugs from natural sources, their production in enzymatic processes and the resolution of racemic mixtures in preparative chromatography are outlined in separate chapters. For the section on qualitative and quantitative analysis, enantioselective chromatographic methods are presented as well as optical methods and CE-MS, while the final section deals with the pharmacology, pharmacokinetics and metabolic aspects of chiral drugs, devoting whole chapters to stereoselective drug binding and modeling chiral drug-receptor interactions. With its unique industry-relevant aspects, this is a must for medicinal and pharmaceutical chemists.

Chiral Chromatography

Chiral Chromatography
Author: Thomas E. Beesley,Raymond P. W. Scott
Publsiher: John Wiley & Sons
Total Pages: 520
Release: 1999-01-26
ISBN 10: 9780471974277
ISBN 13: 0471974277
Language: EN, FR, DE, ES & NL

Chiral Chromatography Book Review:

Chiral Chromatography Thomas E. Beesley Advanced Separation Technologies Inc., Whippany, New Jersey, USA Raymond P. W. Scott Chemistry Department, Georgetown University, Washington DC, USA and Chemistry Department, Birkbeck College, University of London, UK Analytical techniques based on separation processes, such as chromatography and electrophoresis, are finding a growing range of applications in chemical, pharmaceutical and clinical laboratories. The Wiley Separation Science Series provides the analyst in these laboratories with well-focused books covering individual techniques, so that they can be applied more efficiently and effectively to contemporary analytical problems. The different enantiomers of a drug can exhibit widely different physiological activity in degree and nature. As a result, the separation and identification of enantiomers is now a very important analytical problem and chiral chromatography is the natural technique to apply to the resolution of such mixtures. Chiral Chromatography provides the reader with a basic understanding of the nature of chromatographic separations and relates the principles specifically to the separation of enantiomers. The following information is included: * chiral separations involving both gas and liquid chromatography * descriptions of the apparatus used for both techniques * detailed discussion on the retention mechanism that results in chiral selectivity * the structure and synthesis of a wide range of chirally active stationary phases used in both gas and liquid chromatography * preparative applications for large scale purification of enantiomers * applications of capillary electrophoresis and capillary electrochromatography. In addition to the above, a large number of examples of the separation of both commercially and physiologically interesting chiral mixtures are given, as is a detailed discussion on the mechanism of selectivity of each example. Thomas Beesley was founder and is the CEO for a leading manufacturer of chiral stationary phases and has published papers on TLC, HPLC and chiral separations involving cyclodextrins. He has also coauthored papers with Daniel W. Armstrong, an expert on modern cyclodextrin columns. Raymond Scott has worked in the field of separation science for over 40 years and has contributed extensively to the development of both gas and liquid chromatography publishing over 160 papers on the subjects.

Molecular Basis of Chromatographic Separation

Molecular Basis of Chromatographic Separation
Author: Esther Forgacs,Tibor Cserhati
Publsiher: CRC Press
Total Pages: 256
Release: 1997-07-03
ISBN 10: 9780849376962
ISBN 13: 0849376963
Language: EN, FR, DE, ES & NL

Molecular Basis of Chromatographic Separation Book Review:

Chromatographic separation is widely used in many scientific disciplines today, having an ever increasing number of scientific and technological applications. The widespread use of this rapid and powerful technique requires that it be fully understood, so that the most suitable may be determined for each possible separation problem in each possible domain of scientific research and technology. Molecular Basis of Chromatographic Separation provides complete coverage of the practical and molecular aspects of this popular technique. It compiles and evaluates recent results, outlines available methods, and discusses how to select the best method for a particular application.

Protein Chromatography Methods and Protocols

Protein Chromatography  Methods and Protocols
Author: Dermot Walls,Sinead T. Loughran
Publsiher: Methods in Molecular Biology
Total Pages: 438
Release: 2018-10-26
ISBN 10: 9781493981915
ISBN 13: 1493981919
Language: EN, FR, DE, ES & NL

Protein Chromatography Methods and Protocols Book Review:

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

Separation Methods in Drug Synthesis and Purification

Separation Methods in Drug Synthesis and Purification
Author: Klara Valko
Publsiher: Elsevier
Total Pages: 612
Release: 2000-10-13
ISBN 10: 9780080541099
ISBN 13: 0080541097
Language: EN, FR, DE, ES & NL

Separation Methods in Drug Synthesis and Purification Book Review:

Separation Methods in Drug Synthesis and Purification

Encyclopedia of Chromatography Print

Encyclopedia of Chromatography  Print
Author: Jack Cazes
Publsiher: CRC Press
Total Pages: 927
Release: 2001-06-29
ISBN 10: 9780824705114
ISBN 13: 0824705114
Language: EN, FR, DE, ES & NL

Encyclopedia of Chromatography Print Book Review:

This practical, single-volume source collects up-to-date information on chromatographic techniques and methodologies for the solution of analytical and preparative problems applicable across a broad spectrum of disciplines including biotechnology, pharmaceuticals, environmental sciences, polymers, food additives and nutrients, pathology, toxicology, fossil fuels, and nuclear chemistry. It highlights real-world applications, easy-to-read fundamentals of problem solving and material identification methods, and detailed references. Written by over 180 esteemed international authorities and containing over 300 chapters, 2600 works cited, and 1000 drawings, equations, tables, and photographs, the Encyclopedia of Chromatography covers high-performance liquid, thin-layer, gas, affinity, countercurrent, supercritical fluid, gel permeation, and size exclusion chromatographies as well as capillary electrophoresis, field-flow fractionation, hyphenated techniques, and more. PRINT/ONLINE PRICING OPTIONS AVAILABLE UPON REQUEST AT [email protected]

Essentials in Modern HPLC Separations

Essentials in Modern HPLC Separations
Author: Serban C. Moldoveanu,Victor David
Publsiher: Newnes
Total Pages: 532
Release: 2012-09-21
ISBN 10: 0123850134
ISBN 13: 9780123850133
Language: EN, FR, DE, ES & NL

Essentials in Modern HPLC Separations Book Review:

This book discusses in a systematic manner the role of separation in HPLC, the types and characteristics of stationary phases and of mobile phases used in this technique, as well as other factors influencing the separation. The selection process of stationary and mobile phase for a specific separation is described as related to the physico-chemical characteristics of the molecules to be separated and of their matrix. All these subjects are discussed from the point of view of the new developments in HPLC. The book also includes a part presenting the practice of modern HPLC as necessary for applications, particularly related to the analysis of pharmaceutical and biological samples, food and beverages, environmental samples, etc. Gives a clear presentation of notions and conceptsDiscusses key parameters in HPLC separationIncludes modern developments in HPLCDescribes interrelation between various HPLC features (solvent pressure, separation, detection)Includes a large number of references.

Electrophoresis

Electrophoresis
Author: Oana-Maria Boldura,Cornel Balta
Publsiher: BoD – Books on Demand
Total Pages: 154
Release: 2018-09-12
ISBN 10: 1789235529
ISBN 13: 9781789235524
Language: EN, FR, DE, ES & NL

Electrophoresis Book Review:

Electrophoresis is a widely used method in the field of life sciences, having multiple practical applications in physical, chemical, biochemical, and molecular biology domains. This book contains 8 chapters depicting various applications of this technique in biochemistry, molecular biology, and physical chemistry. This book presents the link between the exposed method and its applications in a very explicit manner and offers a wide range of practical examples. The book provides not only a vision of actual methods but also their necessary further improvements and suggested developments. Therefore, a particular attention was given to the described techniques as true guidelines in the fields where electrophoresis is recommended, being useful for not only the scientists but also the laboratory clinicians.

Advances in Chromatography

Advances in Chromatography
Author: Eli Grushka,Nelu Grinberg
Publsiher: CRC Press
Total Pages: 328
Release: 2016-04-19
ISBN 10: 1466569662
ISBN 13: 9781466569669
Language: EN, FR, DE, ES & NL

Advances in Chromatography Book Review:

For more than four decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. For Volume 51, the series editors have invited established, well-known chemists from across the globe to offer cutting-edge reviews on their areas of expertise—from theoretical aspects to novel and established applications of chromatographic techniques. Featured topics include Nonequilibrium Thermodynamics in Nonlinear Chromatography and Electrophoresis: Theory and Applications Biomimetic Chromatography: A Useful Tool in the Drug Discovery Process Solid-Phase Microextraction for In Vivo Pharmacokinetics and Other Stages of Drug Development Identification and Detection of Antibiotic Drugs and Their Degradation Products in Aquatic Samples Sample Preparation for Chromatographic Analysis Development of HPLC Retention Index QSAR Models for Nontargeted Metabolomics Thin Layer Chromatography with a Controlled Gas Phase Influencing on the Separation The clear presentation of topics and vivid illustrations for which this series has become known makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill.

HPLC Method Development for Pharmaceuticals

HPLC Method Development for Pharmaceuticals
Author: Satinder Ahuja,Henrik Rasmussen
Publsiher: Elsevier
Total Pages: 532
Release: 2011-09-21
ISBN 10: 0080554199
ISBN 13: 9780080554198
Language: EN, FR, DE, ES & NL

HPLC Method Development for Pharmaceuticals Book Review:

High pressure, or high performance, liquid chromatography (HPLC) is the method of choice for checking purity of new drug candidates, monitoring changes during scale up or revision of synthetic procedures, evaluating new formulations, and running control/assurance of the final drug product. HPLC Method Development for Pharmaceuticals provides an extensive overview of modern HPLC method development that addresses these unique concerns. Includes a review and update of the current state of the art and science of HPLC, including theory, modes of HPLC, column chemistry, retention mechanisms, chiral separations, modern instrumentation (including ultrahigh-pressure systems), and sample preparation. Emphasis has been placed on implementation in a pharmaceutical setting and on providing a practical perspective. HPLC Method Development for Pharmaceuticals is intended to be particularly useful for both novice and experienced HPLC method development chemists in the pharmaceutical industry and for managers who are seeking to update their knowledge. Covers the requirements for HPLC in a pharmaceutical setting including strategies for software and hardware validation to allow for use in a regulated laboratory Provides an overview of the pharmaceutical development process (clinical phases, chemical and pharmaceutical development activities) Discusses how HPLC is used in each phase of pharmaceutical development and how methods are developed to support activities in each phase

Introduction to Pharmaceutical Chemical Analysis

Introduction to Pharmaceutical Chemical Analysis
Author: Steen Honoré Hansen,Stig Pedersen-Bjergaard,Knut Rasmussen
Publsiher: John Wiley & Sons
Total Pages: 624
Release: 2011-10-18
ISBN 10: 1119954339
ISBN 13: 9781119954330
Language: EN, FR, DE, ES & NL

Introduction to Pharmaceutical Chemical Analysis Book Review:

This textbook is the first to present a systematic introduction to chemical analysis of pharmaceutical raw materials, finished pharmaceutical products, and of drugs in biological fluids, which are carried out in pharmaceutical laboratories worldwide. In addition, this textbook teaches the fundamentals of all the major analytical techniques used in the pharmaceutical laboratory, and teaches the international pharmacopoeias and guidelines of importance for the field. It is primarily intended for the pharmacy student, to teach the requirements in “analytical chemistry” for the 5 years pharmacy curriculum, but the textbook is also intended for analytical chemists moving into the field of pharmaceutical analysis. Addresses the basic concepts, then establishes the foundations for the common analytical methods that are currently used in the quantitative and qualitative chemical analysis of pharmaceutical drugs Provides an understanding of common analytical techniques used in all areas of pharmaceutical development Suitable for a foundation course in chemical and pharmaceutical sciences Aimed at undergraduate students of degrees in Pharmaceutical Science/Chemistry Analytical Science/Chemistry, Forensic analysis Includes many illustrative examples

Supercritical Fluid Chromatography

Supercritical Fluid Chromatography
Author: Gregory K. Webster
Publsiher: CRC Press
Total Pages: 418
Release: 2014-02-04
ISBN 10: 9814463000
ISBN 13: 9789814463003
Language: EN, FR, DE, ES & NL

Supercritical Fluid Chromatography Book Review:

Analytical chemists in the pharmaceutical industry are always looking for more-efficient techniques to meet the analytical challenges of today’s pharmaceutical industry. One technique that has made steady advances in pharmaceutical analysis is supercritical fluid chromatography (SFC). SFC is meeting the chromatography needs of the industry by providing efficient and selective testing capabilities on the analytical and preparative scale. The supercritical fluid mobile phase, consisting mainly of CO2, facilitates cost reduction costs and helps the industry in meeting green chemistry standards. This book provides a comprehensive overview of the use of SFC in pharmaceutical analysis. Supercritical Fluid Chromatography reviews the use of SFC in drug-discovery applications and describes its application in drug development. When a drug is developed and brought to market, it is tested many times for impurities and degradants, enantiomeric purity, and analytical and preparative isolations—it is tested during discovery and development and for under-regulated and unregulated methodologies. The book describes the use of SFC for each of these applications and discusses more in-depth topics, such as the use of SFC in mass spectrometric and polarographic detection. The book also sheds light on the role of SFC in drug development from natural products and the advancement of SFC with new technologies and its use in pilot-scale operations as a chromatographic technique.

Chiral Separation Methods for Pharmaceutical and Biotechnological Products

Chiral Separation Methods for Pharmaceutical and Biotechnological Products
Author: Satinder Ahuja
Publsiher: John Wiley & Sons
Total Pages: 400
Release: 2011-03-31
ISBN 10: 9781118097755
ISBN 13: 1118097750
Language: EN, FR, DE, ES & NL

Chiral Separation Methods for Pharmaceutical and Biotechnological Products Book Review:

Discusses chiral separations and offers guidance for selectingthe optimum method for desired results Chiral separations represent the most intriguing and, by somemeasures, most difficultseparations of chemical compounds. Thisbook provides researchers and students an under-standing of chiralseparations and offers a convenient route to selecting the bestseparation method, saving considerable time and cost in productdevelopment. Considering chiral separations in the biotechnological andpharmaceutical industries, as well as for food applications, Dr.Ahuja provides insights into a broad range of topics. Opening witha broad overview of chiral separations, regulatory considerationsin drug product development, and basic issues in methoddevelopment, the book: Covers a variety of modern methods such as gas chromatography,high performance liquid chromatography, supercritical fluidchromatography, and capillary electrophoresis Deals with the impact of chirality on the biological activity ofsmall and large molecules Provides detailed information on useful chiral stationary phases(CSPs) for HPLC Includes handy information on selection of an appropriate CSP,including mechanistic studies Offers strategies for fast method development with HPLC, SFC,and CE Discusses preparatory methods utilized in the pharmaceuticalindustry With in-depth discussions of the current state of the field aswell as suggestions to assist future developments, ChiralSeparation Methods for Pharmaceutical and BiotechnologicalProducts is an essential text for laboratory investigators,managers, and regulators who are involved in chiral separations inthe pharmaceutical industry, as well as students preparing forcareers in these fields.

Preparative Chromatography

Preparative Chromatography
Author: H. Schmidt-Traub
Publsiher: John Wiley & Sons
Total Pages: 485
Release: 2006-03-06
ISBN 10: 3527605908
ISBN 13: 9783527605903
Language: EN, FR, DE, ES & NL

Preparative Chromatography Book Review:

This interdisciplinary approach combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. The book covers recent developments in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on solid materials and packed columns process concepts. Final chapters on modeling and determination of model parameters, the design and optimization of preparative chromatographic processes and chromatographic reactors allow for the optimum selection of chromatographic systems. Essential for chemists and engineers working in the chemicals and pharmaceutical industries as well as for food technologies, due to the interdisciplinary nature of these processes.