Mass Spectrometry in Biophysics

Mass Spectrometry in Biophysics
Author: Igor A. Kaltashov,Stephen J. Eyles
Publsiher: John Wiley & Sons
Total Pages: 320
Release: 2005-05-06
ISBN 10: 0471705160
ISBN 13: 9780471705161
Language: EN, FR, DE, ES & NL

Mass Spectrometry in Biophysics Book Review:

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

Mass Spectrometry in Structural Biology and Biophysics

Mass Spectrometry in Structural Biology and Biophysics
Author: Igor A. Kaltashov,Stephen J. Eyles
Publsiher: John Wiley & Sons
Total Pages: 289
Release: 2012-04-03
ISBN 10: 0470937793
ISBN 13: 9780470937792
Language: EN, FR, DE, ES & NL

Mass Spectrometry in Structural Biology and Biophysics Book Review:

With its detailed and systematic coverage of the current state of biophysical mass spectrometry (MS), here is one of the first systematic presentations of the full experimental array of MS-based techniques used in biophysics, covering both fundamental and practical issues. The book presents a discussion of general biophysical concepts and a brief overview of traditional biophysical techniques before outlining the more advanced concepts of mass spectrometry. The new edition gives an up-to-date and expanded coverage of experimental methodologies and a clear look at MS-based methods for studying higher order structures and biopolymers. A must for researchers in the field of biophysics, structural biology, and protein chemistry.

Biophysical Techniques in Drug Discovery

Biophysical Techniques in Drug Discovery
Author: Angeles Canales
Publsiher: Royal Society of Chemistry
Total Pages: 320
Release: 2017-11-14
ISBN 10: 1788012860
ISBN 13: 9781788012867
Language: EN, FR, DE, ES & NL

Biophysical Techniques in Drug Discovery Book Review:

Biophysical techniques are used in many key stages of the drug discovery process including in screening for new receptor ligands, in characterising drug mechanisms, and in validating data from biochemical and cellular assays. This book provides an overview of the biophysical methods applied in drug discovery today, including traditional techniques and newer developments. Perspectives from academia and industry across a spectrum of techniques are brought together in a single volume. Small and biotherapeutic approaches are covered and strengths and limitations of each technique are presented. Case studies illustrate the application of each technique in real applied examples. Finally, the book covers recent developments in areas such as electron microscopy with discussions of their possible impact on future drug discovery. This is a go-to volume for biophysicists, analytical chemists and medicinal chemists providing a broad overview of techniques of contemporary interest in drug discovery.

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Biophysical Characterization of Proteins in Developing Biopharmaceuticals
Author: Damian J. Houde,Steven A. Berkowitz
Publsiher: Elsevier
Total Pages: 586
Release: 2019-11-13
ISBN 10: 0444641742
ISBN 13: 9780444641748
Language: EN, FR, DE, ES & NL

Biophysical Characterization of Proteins in Developing Biopharmaceuticals Book Review:

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical’s developability and the technical decision-making process needed when dealing with biophysical characterization data. Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development Highlights the capabilities and limitations of each technique Discusses the underlining science of each tool Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry

Introduction to Biophysical Methods for Protein and Nucleic Acid Research

Introduction to Biophysical Methods for Protein and Nucleic Acid Research
Author: Jay A. Glasel,Murray P. Deutscher
Publsiher: Academic Press
Total Pages: 510
Release: 1995-11-20
ISBN 10: 9780080534985
ISBN 13: 0080534988
Language: EN, FR, DE, ES & NL

Introduction to Biophysical Methods for Protein and Nucleic Acid Research Book Review:

The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment. Key Features * Modern Use of Mass Spectroscopy * NMR Spectroscopy * Molecular Modeling and Graphics * Macintosh and DOS/Windows 3.x disks

Methods in Modern Biophysics

Methods in Modern Biophysics
Author: Bengt Nölting
Publsiher: Springer Science & Business Media
Total Pages: 254
Release: 2013-03-09
ISBN 10: 3662053675
ISBN 13: 9783662053676
Language: EN, FR, DE, ES & NL

Methods in Modern Biophysics Book Review:

Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

Modern Biophysical Chemistry

Modern Biophysical Chemistry
Author: Peter Jomo Walla
Publsiher: John Wiley & Sons
Total Pages: 360
Release: 2015-09-10
ISBN 10: 3527683550
ISBN 13: 9783527683550
Language: EN, FR, DE, ES & NL

Modern Biophysical Chemistry Book Review:

This updated and up-to-date version of the first edition continues with the really interesting stuff to spice up a standard biophysics and biophysical chemistry course. All relevant methods used in current cutting edge research including such recent developments as super-resolution microscopy and next-generation DNA sequencing techniques, as well as industrial applications, are explained. The text has been developed from a graduate course taught by the author for several years, and by presenting a mix of basic theory and real-life examples, he closes the gap between theory and experiment. The first part, on basic biophysical chemistry, surveys fundamental and spectroscopic techniques as well as biomolecular properties that represent the modern standard and are also the basis for the more sophisticated technologies discussed later in the book. The second part covers the latest bioanalytical techniques such as the mentioned super-resolution and next generation sequencing methods, confocal fluorescence microscopy, light sheet microscopy, two-photon microscopy and ultrafast spectroscopy, single molecule optical, electrical and force measurements, fluorescence correlation spectroscopy, optical tweezers, quantum dots and DNA origami techniques. Both the text and illustrations have been prepared in a clear and accessible style, with extended and updated exercises (and their solutions) accompanying each chapter. Readers with a basic understanding of biochemistry and/or biophysics will quickly gain an overview of cutting edge technology for the biophysical analysis of proteins, nucleic acids and other biomolecules and their interactions. Equally, any student contemplating a career in the chemical, pharmaceutical or bio-industry will greatly benefit from the technological knowledge presented. Questions of differing complexity testing the reader's understanding can be found at the end of each chapter with clearly described solutions available on the Wiley-VCH textbook homepage under: www.wiley-vch.de/textbooks

Applied Biophysics for Drug Discovery

Applied Biophysics for Drug Discovery
Author: Donald Huddler,Edward R. Zartler
Publsiher: John Wiley & Sons
Total Pages: 312
Release: 2017-10-02
ISBN 10: 111909948X
ISBN 13: 9781119099482
Language: EN, FR, DE, ES & NL

Applied Biophysics for Drug Discovery Book Review:

13.2.1 Protein Dynamics of SA WT and S1 Mutant DHFR in Apo and Bound States

Biophysical Methods for Biotherapeutics

Biophysical Methods for Biotherapeutics
Author: Tapan K. Das
Publsiher: Wiley
Total Pages: 376
Release: 2014-04-28
ISBN 10: 0470938439
ISBN 13: 9780470938430
Language: EN, FR, DE, ES & NL

Biophysical Methods for Biotherapeutics Book Review:

With a focus on practical applications of biophysical techniques, this book links fundamental biophysics to the process of biopharmaceutical development. • Helps formulation and analytical scientists in pharma and biotech better understand and use biophysical methods • Chapters organized according to the sequential nature of the drug development process • Helps formulation, analytical, and bioanalytical scientists in pharma and biotech better understand and usestrengths and limitations of biophysical methods • Explains how to use biophysical methods, the information obtained, and what needs to be presented in a regulatory filing, assess impact on quality and immunogenicity • With a focus on practical applications of biophysical techniques, this book links fundamental biophysics to the process of biopharmaceutical development.

Biophysical Approaches Determining Ligand Binding to Biomolecular Targets

Biophysical Approaches Determining Ligand Binding to Biomolecular Targets
Author: Alberto Podjarny,Annick P Dejaegere,Bruno Kieffer
Publsiher: Royal Society of Chemistry
Total Pages: 260
Release: 2011-04-01
ISBN 10: 1849732663
ISBN 13: 9781849732666
Language: EN, FR, DE, ES & NL

Biophysical Approaches Determining Ligand Binding to Biomolecular Targets Book Review:

The binding of small ligands to biological molecules is central to most aspects of biological function. The past twenty years has seen the development of an increasing armoury of biophysical methods that not only detect such binding, but also provide varying degrees of information about the kinetics, thermodynamics and structural aspects of the process. These methods have received increasing attention with the growth in more rational approaches to drug discovery and design. This book reviews the latest advances in the application of biophysics to the study of ligand binding. It provides a complete overview of current techniques to identify ligands, characterise their binding sites and understand their binding mechanisms. Particular emphasis is given to the combined use of different techniques and their relative strengths and weaknesses. Consistency in the way each technique is described makes it easy for readers to select the most suitable protocol for their research. The introduction explains why some techniques are more suitable than others and emphasizes the possible synergies between them. The following chapters, all written by a specialist in the particular technique, focus on each method individually. The book finishes by describing how several complimentary techniques can be used together for maximum effectiveness. This book is suitable for biomolecular scientists at graduate or post-doctoral level in academia and industry. Biologists and chemists will also find it a useful introduction to the techniques available.

Biophysical Techniques in Photosynthesis

Biophysical Techniques in Photosynthesis
Author: Thijs Aartsma,Jörg Matysik
Publsiher: Springer Science & Business Media
Total Pages: 520
Release: 2008-02-01
ISBN 10: 1402082509
ISBN 13: 9781402082504
Language: EN, FR, DE, ES & NL

Biophysical Techniques in Photosynthesis Book Review:

Since the first volume on Biophysical Techniques in Photosynthesis Research, published in 1996, new experimental techniques and methods have been devised at a rapid pace. The present book is a sequel which complements the publication of the first volume by providing a comprehensive overview of the most important new techniques developed over the past ten years, especially those that are relevant for research on the mechanism and fundamental aspects of photosynthesis.

Biophysical Chemistry

Biophysical Chemistry
Author: Alan Cooper
Publsiher: Royal Society of Chemistry
Total Pages: 184
Release: 2004
ISBN 10: 9780854044801
ISBN 13: 0854044809
Language: EN, FR, DE, ES & NL

Biophysical Chemistry Book Review:

This book will be ideal for early undergraduates studying chemical or physical sciences and will act as a basis for more advanced study.

Ions in Water and Biophysical Implications

Ions in Water and Biophysical Implications
Author: Yizhak Marcus
Publsiher: Springer Science & Business Media
Total Pages: 216
Release: 2012-08-13
ISBN 10: 9400746474
ISBN 13: 9789400746473
Language: EN, FR, DE, ES & NL

Ions in Water and Biophysical Implications Book Review:

Over the past decade, numerous books have attempted to explain ions in aqueous solutions in relation to biophysical phenomena. Ions in Water and Biophysical Implications, from Chaos to Cosmos offers a physicochemical point of view of the spread of this matter and suggests innovative solutions that will challenge the biophysics research establishment. Starting with a throughout discussion of the properties of liquid water, in particular as a structured liquid with an extensive hydrogen bonded structure, the book examines water as a solvent for gases, non-electrolytes, and electrolytes and reviews the properties, sizes and thermodynamics of isolated and aqueous ions, as well as their interactions, including those of polyelectrolytes. The effects of ions on water structure, including those on solvent dynamics and certain thermodynamic quantities, are presented. This volume investigates water surfaces with its vapour, with another liquid, and with a solid, as well as the effects of solutes, including simple ions and the water-miscible non-electrolytes. Surfaces are relevant to biomolecular and colloidal systems and the book discusses briefly surfactants, micelles and vesicles. Finally, the book concludes with a review of the various biophysical implications involving chaotropic and kosmotropic ions in homogeneous solutions and the Hofmeister series for ions concerning biomolecular and colloidal systems and some aspects of protein hydration and K+/Na+ selectivity in ion channels. Ions in Water and Biophysical Implications, from Chaos to Cosmos will appeal to physical chemists, biophysicists, biochemists, as well as to all students and researchers involved in the study of aqueous solutions.

Methods in Molecular Biophysics

Methods in Molecular Biophysics
Author: Nathan R. Zaccai,Igor N. Serdyuk,Joseph Zaccai
Publsiher: Cambridge University Press
Total Pages: 702
Release: 2017-05-18
ISBN 10: 1107056373
ISBN 13: 9781107056374
Language: EN, FR, DE, ES & NL

Methods in Molecular Biophysics Book Review:

A comprehensive graduate textbook explaining key physical methods in biology, reflecting the very latest research in this fast-moving field.

Biomedical Applications of Biophysics

Biomedical Applications of Biophysics
Author: Thomas Jue
Publsiher: Springer Science & Business Media
Total Pages: 237
Release: 2010-08-18
ISBN 10: 9781603272339
ISBN 13: 160327233X
Language: EN, FR, DE, ES & NL

Biomedical Applications of Biophysics Book Review:

In keeping with goal and style of the Handbook in Modern Biophysics series, the proposed book will maintain a chapter structure that contains two parts: concepts and biological application. The book also integrates all the chapters into a smooth, continuous discourse. The first and second chapters establish the mathematical methods and theoretical framework underpinning the different topics in the rest if the book. Other chapters will use the theoretical framework as a basis to discuss optical and NMR approaches. Each chapter will contain innovative didactic elements that facilitate teaching, self-study, and research preparation (key points, summary, exercise, references).

Introduction to Biomolecular Structure and Biophysics

Introduction to Biomolecular Structure and Biophysics
Author: Gauri Misra
Publsiher: Springer
Total Pages: 274
Release: 2017-09-07
ISBN 10: 9811049688
ISBN 13: 9789811049682
Language: EN, FR, DE, ES & NL

Introduction to Biomolecular Structure and Biophysics Book Review:

This comprehensive book presents a modern concept in biophysics based on recently published research. It highlights various aspects of the biophysical fundamentals and techniques that are currently used to study different physical properties of biomolecules, and relates the biological phenomenon with the underlying physical concepts. The content is divided into nine chapters summarizing the structural details of proteins, including recently discovered novel folds, higher order structures of nucleic acids, as well as lipids and the physical forces governing the macromolecular interactions which are essential for the various biological processes. It also provides insights into the recent advances in biophysical techniques including Hydrogen Deuterium Exchange with Mass Spectrometry (HDX-MS), Small angle X-ray scattering (SAXS) and Cryo Electron Microscopy (cryo EM), supplemented with interesting experimental data. It is a valuable reference resource for anyone with a desire to gain a better understanding of the fundamentals of biophysical concepts and techniques of important biomolecules.

Advances in Ion Mobility Mass Spectrometry Fundamentals Instrumentation and Applications

Advances in Ion Mobility Mass Spectrometry  Fundamentals  Instrumentation and Applications
Author: Anonim
Publsiher: Elsevier
Total Pages: 278
Release: 2019-01-10
ISBN 10: 0444641556
ISBN 13: 9780444641557
Language: EN, FR, DE, ES & NL

Advances in Ion Mobility Mass Spectrometry Fundamentals Instrumentation and Applications Book Review:

Ion Mobility Spectrometry, Volume 83 will focuses on new trends, methods and instrumentation in the field, starting from the innovations of each technique, to the most progressive challenges of IM-MS. Chapters includes section on Recent advances in IM-MS, IM-MS Principles and Theory, IM-MS Applications and Instrumentation, and the Future of IM-MS. Presents the latest advancements in IM-MS that are essential for new applications Helps readers understand the state-of-the-art in the currently available IM-MS interfaces and their principle uses Provides information on different IM-MS instrumentation Delves into key applications of IM-MS

Mass Spectrometry Based Chemical Proteomics

Mass Spectrometry Based Chemical Proteomics
Author: W. Andy Tao,Ying Zhang
Publsiher: John Wiley & Sons
Total Pages: 448
Release: 2019-07-10
ISBN 10: 1118970217
ISBN 13: 9781118970218
Language: EN, FR, DE, ES & NL

Mass Spectrometry Based Chemical Proteomics Book Review:

PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Biophysical Techniques for Structural Characterization of Macromolecules

Biophysical Techniques for Structural Characterization of Macromolecules
Author: Edward H. Egelman,H. Jane Dyson
Publsiher: Unknown
Total Pages: 617
Release: 2012
ISBN 10: 9780123749208
ISBN 13: 0123749204
Language: EN, FR, DE, ES & NL

Biophysical Techniques for Structural Characterization of Macromolecules Book Review:

Hydrogen Exchange Mass Spectrometry of Proteins

Hydrogen Exchange Mass Spectrometry of Proteins
Author: David D. Weis
Publsiher: John Wiley & Sons
Total Pages: 376
Release: 2016-03-21
ISBN 10: 1118616499
ISBN 13: 9781118616499
Language: EN, FR, DE, ES & NL

Hydrogen Exchange Mass Spectrometry of Proteins Book Review:

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.